Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0.1 mM, no resiudal activity | Phanerodontia chrysosporium | |
Cu2+ | 0.1 mM, no resiudal activity | Phanerodontia chrysosporium | |
Hg2+ | 0.1 mM, no resiudal activity | Phanerodontia chrysosporium | |
additional information | not inhibitory: EDTA, Mg2+, Ca2+, iodoacetamide | Phanerodontia chrysosporium | |
Phenylmercuriacetate | 0.01 mM, no resiudal activity | Phanerodontia chrysosporium | |
S-adenosyl-L-homocysteine | product inhibition | Phanerodontia chrysosporium | |
Zn2+ | 0.1 mM, 45% resiudal activity | Phanerodontia chrysosporium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.034 | - |
4-hydroxy-3-methoxyacetophenone | pH 8.0, 37°C | Phanerodontia chrysosporium | |
0.099 | - |
S-adenosyl-L-methionine | pH 8.0, 37°C | Phanerodontia chrysosporium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
gel filtration | Phanerodontia chrysosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Phanerodontia chrysosporium |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
101 | - |
pH 8.0, 37°C | Phanerodontia chrysosporium |
Storage Stability | Organism |
---|---|
-4°C, 100 mM potassium phosphate buffer, pH 6.5, 1 mM dithiothreitol, 75% loss of activity, in presence of 10% glycerol 5% loss of activtiy | Phanerodontia chrysosporium |
2°C, 100 mM potassium phosphate buffer, pH 6.5, 1 mM dithiothreitol, a half-life of approximately 30 h | Phanerodontia chrysosporium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | 3-methoxy- and 3,5-dimethoxy-substituted 4-hydroxy-benzaldehydes, -benzoic acids, and -acetophenones are the preferred substrates. The corresponding 3,4-dihydroxy compounds are methylated relatively slowly, while the 3-hydroxy-4-methoxy compounds are almost inactive as substrates. Substituents in both the 2 and 4 positions relative to the hydroxyl group appear to be essential for significant enzyme attack of a substrate. Xenobiotic compounds such as 2,4-dichlorophenol and 2,4-dibromophenol are methylated almost as readily as 4-hydroxy-3-methoxyacetophenone. No substrates: homovanillic acid, caffeic acid, ferulic acid | Phanerodontia chrysosporium | ? | - |
? | |
S-adenosyl-L-methionine + 2,4-dibromophenol | - |
Phanerodontia chrysosporium | S-adenosyl-L-homocysteine + 2,4-dibromo-1-methoxybenzene | - |
? | |
S-adenosyl-L-methionine + 2,4-dichlorophenol | - |
Phanerodontia chrysosporium | S-adenosyl-L-homocysteine + 2,4-dichloro-1-methoxybenzene | - |
? | |
S-adenosyl-L-methionine + 4-hydroxy-3,5-dimethoxyacetophenone | - |
Phanerodontia chrysosporium | S-adenosyl-L-homocysteine + 3,4,5-trimethoxyacetophenone | - |
? | |
S-adenosyl-L-methionine + 4-hydroxy-3,5-dimethoxybenzaldehyde | - |
Phanerodontia chrysosporium | S-adenosyl-L-homocysteine + 3,4,5-trimethoxybenzaldehyde | - |
? | |
S-adenosyl-L-methionine + 4-hydroxy-3,5-dimethoxybenzoic acid | - |
Phanerodontia chrysosporium | S-adenosyl-L-homocysteine + 3,4,5-trimethoxybenzoic acid | - |
? | |
S-adenosyl-L-methionine + 4-hydroxy-3-methoxyacetophenone | - |
Phanerodontia chrysosporium | S-adenosyl-L-homocysteine + 3,4-dimethoxyacetophenone | - |
? | |
S-adenosyl-L-methionine + 4-hydroxy-3-methoxybenzaldehyde | - |
Phanerodontia chrysosporium | S-adenosyl-L-homocysteine + 3,4-dimethoxybenzaldehyde | - |
? | |
S-adenosyl-L-methionine + 4-hydroxy-3-methoxybenzoic acid | - |
Phanerodontia chrysosporium | S-adenosyl-L-homocysteine + 3,4-dimethoxybenzoic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 54000, SDS-PAGE | Phanerodontia chrysosporium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
rapid inactivation above | Phanerodontia chrysosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Phanerodontia chrysosporium |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.041 | - |
S-adenosyl-L-homocysteine | pH 8.0, 37°C | Phanerodontia chrysosporium |