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Literature summary for 2.1.1.248 extracted from

  • Soares, J.; Zhang, L.; Pitsch, R.; Kleinholz, N.; Jones, R.; Wolff, J.; Amster, J.; Green-Church, K.; Krzyck, J.
    The residue mass of L-pyrrolysine in three distinct methylamine methyltransferases (2005), J. Biol. Chem., 280, 36962-36969.
    View publication on PubMed
Search for more literature for 2.1.1.248

Cloned(Commentary)

Cloned (Comment) Organism
gene mtmB Methanosarcina barkeri

Crystallization (Commentary)

Crystallization (Comment) Organism
crystal structure determination and analysis Methanosarcina barkeri

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 x * 50000, SDS-PAGE Methanosarcina barkeri

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methylamine + a [Co(I) methylamine-specific corrinoid protein] Methanosarcina barkeri
-
 a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
 ?
methylamine + a [Co(I) methylamine-specific corrinoid protein] Methanosarcina barkeri MS / DSM 800
-
 a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
 ?

Organism

Organism UniProt Comment Textmining
Methanosarcina barkeri O30642 gene mtmB1; gene mtmB1
-
Methanosarcina barkeri Q9P9L4 gene mtmB2; gene mtmB2
-
Methanosarcina barkeri MS / DSM 800 O30642 gene mtmB1; gene mtmB1
-
Methanosarcina barkeri MS / DSM 800 Q9P9L4 gene mtmB2; gene mtmB2
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme, separation from co-purifiying MtmC, the methylamine-specific corrinoid protein, by gel filtration Methanosarcina barkeri

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methylamine + a [Co(I) methylamine-specific corrinoid protein]
-
 Methanosarcina barkeri a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
 ?
methylamine + a [Co(I) methylamine-specific corrinoid protein]
-
 Methanosarcina barkeri MS / DSM 800 a [methyl-Co(III) methylamine-specific corrinoid protein] + ammonia
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Methanosarcina barkeri
More mass spectrometric structure analysis, overview, primary structure of MtmB Methanosarcina barkeri

Synonyms

Synonyms Comment Organism
MtmB
-
 Methanosarcina barkeri
mtmB1
-
 Methanosarcina barkeri
mtmB2
-
 Methanosarcina barkeri

General Information

General Information Comment Organism
additional information single in-frame amber UAG codons are found in the genes encoding MtmB, MtbB, or MttB, the methyltransferases initiating methane formation from monomethylamine, dimethylamine, or trimethylamine, respectively, in certain Archaea. The amber codon codes for pyrrolysine, the 22nd genetically encoded amino acid found in nature Methanosarcina barkeri