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Literature summary for 2.1.1.244 extracted from

  • Richardson, S.L.; Mao, Y.; Zhang, G.; Hanjra, P.; Peterson, D.L.; Huang, R.
    Kinetic mechanism of protein N-terminal methyltransferase 1 (2015), J. Biol. Chem., 290, 11601-11610.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.244

Application

Application Comment Organism
drug development the enzyme is a target for developing specific inhibitors Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes NTMT1 with a thrombin cleavage site at the N-terminus in Escherichia coli strain BL21(DE3) codon plus RIL Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information the inhibition patterns indicate a rapid equilibrium random kinetic mechanism Homo sapiens
N-terminal-acetyl-SPKRIAKRRS-[RCC1] noncompetitive versus S-adenosyl-L-methionine and protein substrate Homo sapiens
N-terminal-trimethyl-SPKRIA-[RCC1] product inhibition, competitive inhibitor versus protein substrate, noncompetitive versus S-adenosyl-L-methionine Homo sapiens
S-adenosyl-L-homocysteine product inhibition, competitive inhibitor versus S-adenosyl-L-methionine, noncompetitive versus protein substrate Homo sapiens
sinefungin noncompetitive versus S-adenosyl-L-methionine and protein substrate Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten model, steady-state kinetic analysis and mechanism Homo sapiens
0.00089
-
 N-terminal-SPKRIAKRRS-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
0.0014
-
 N-terminal-SPKRIAKRR-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
0.0014
-
 N-terminal-methyl-SPKRIAKRRS-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
0.0031
-
 N-terminal-SPKRIAKRRSPP-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
0.0032
-
 N-terminal-SPKRIA-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
0.0043
-
 N-terminal-dimethyl-SPKRIAKRRS-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
0.0049
-
 N-terminal-SPKRIAKRRSPP-[RCC1] recombinant His6-tagged enzyme, pH 7.5, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminally His6-tagged wild-type and mutant enzymes NTMT1 from Escherichia coli strain BL21(DE3) codon plus RIL by nickel affinity chromatography, tag cleavage through thrombin, another 3 steps of nickel affinity chromatography and dialysis Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3 S-adenosyl-L-methionine + N-terminal-dimethyl-SPKRIAKRRS-[RCC1]
-
 Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-SPKRIAKRRS-[RCC1]
-
 ?
3 S-adenosyl-L-methionine + N-terminal-methyl-SPKRIAKRRS-[RCC1]
-
 Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-SPKRIAKRRS-[RCC1]
-
 ?
3 S-adenosyl-L-methionine + N-terminal-SPKRIA-[RCC1]
-
 Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-SPKRIA-[RCC1]
-
 ?
3 S-adenosyl-L-methionine + N-terminal-SPKRIAKRR-[RCC1]
-
 Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-SPKRIAKRR-[RCC1]
-
 ?
3 S-adenosyl-L-methionine + N-terminal-SPKRIAKRRS-[RCC1]
-
 Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-SPKRIAKRRS-[RCC1]
-
 ?
3 S-adenosyl-L-methionine + N-terminal-SPKRIAKRRSPP-[RCC1]
-
 Homo sapiens 3 S-adenosyl-L-homocysteine + N-terminal-trimethyl-SPKRIAKRRSPP-[RCC1]
-
 ?
additional information enzyme NTMT1 catalyzes the transfer of the methyl group from the S-adenosyl-L-methionine to the protein alpha-amine, resulting in formation of S-adenosyl-L-homocysteine and alpha-N-methylated proteins. Inhibition pattern and methylation progress analyses are performed to determine the kinetic mechanism and processivity of NTMT1, the enzyme NTMT1 utilizes a random sequential bi bi mechanism and proceeds in a distributive manner. Residues of RCC1, i.e. regulator of chromosome condensation 1, are recognized by the enzyme. Methylation status of products is analyzed by MALDI-mass spectrometry Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
NTMT1
-
 Homo sapiens
protein N-terminal methyltransferase 1
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0073
-
 N-terminal-SPKRIAKRRS-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
0.0088
-
 N-terminal-SPKRIAKRR-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
0.0093
-
 N-terminal-SPKRIA-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
0.0095
-
 N-terminal-SPKRIAKRRSPP-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
0.0097
-
 N-terminal-methyl-SPKRIAKRRS-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
0.0098
-
 N-terminal-dimethyl-SPKRIAKRRS-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
0.013
-
 N-terminal-SPKRIAKRRSPP-[RCC1] recombinant His6-tagged enzyme, pH 7.5, 37°C Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Homo sapiens

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
additional information
-
 additional information inhibition kinetics, overview Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.28
-
 N-terminal-dimethyl-SPKRIAKRRS-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
2.65
-
 N-terminal-SPKRIAKRRSPP-[RCC1] recombinant His6-tagged enzyme, pH 7.5, 37°C Homo sapiens
2.91
-
 N-terminal-SPKRIA-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
3.07
-
 N-terminal-SPKRIAKRRSPP-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
6.29
-
 N-terminal-SPKRIAKRR-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
6.93
-
 N-terminal-methyl-SPKRIAKRRS-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens
8.2
-
 N-terminal-SPKRIAKRRS-[RCC1] recombinant detagged enzyme, pH 7.5, 37°C Homo sapiens