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Literature summary for 2.1.1.241 extracted from

  • Jonczyk, R.; Schmidt, H.; Osterrieder, A.; Fiesselmann, A.; Schullehner, K.; Haslbeck, M.; Sicker, D.; Hofmann, D.; Yalpani, N.; Simmons, C.; Frey, M.; Gier, A.
    Elucidation of the final reactions of DIMBOA-glucoside biosynthesis in maize: Characterization of Bx6 and Bx7 (2008), Plant Physiol., 146, 1053-1063.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.241

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, Bx7 maps to the cluster of Bx genes on the short arm of chromosome 4, Bx7 is located 9 cM distal to the marker adh2 at position 47, phylogenetic analysis, expression as His-tagged enzyme in Escherichia coli Zea mays

Inhibitors

Inhibitors Comment Organism Structure
additional information 1 mM Mg2+, 1 mM EDTA, 5% ethanol, or 6% DMSO have no effect on BX7 activity Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetic constants of BX7 Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ is not required for catalysis Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, native enzyme, SDS-PAGE Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside Zea mays
-
 S-adenosyl-L-homocysteine + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
-
 ?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Zea mays

Source Tissue

Source Tissue Comment Organism Textmining
seedling Bx7 is highest expressed in seedling tissue Zea mays
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the only substance that serves as a substrate for the recombinant Bx7 is TRIBOA-Glc, neither the aglucone TRIBOA nor well-known substrates of plant O-methyltransferases are efficiently converted by the enzyme. In case of quercetin, a minor activity is detected. BX7 has no catalytic activity toward phenylpropanoids and flavonoids, e.g. apigenin is no substrate Zea mays ?
-
 ?
S-adenosyl-L-methionine + (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
-
 Zea mays S-adenosyl-L-homocysteine + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
-
 ?
S-adenosyl-L-methionine + (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside conversion of (2R)-2,4,7-trihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside, TRIBOA-Glc, to DIMBOA-Glc Zea mays S-adenosyl-L-homocysteine + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside i.e. DIMBOA-Glc ?

Subunits

Subunits Comment Organism
? x * 40000, native enzyme, SDS-PAGE Zea mays
More peptide mapping by mass spectrometry Zea mays

Synonyms

Synonyms Comment Organism
BENZOXAZINLESS7
-
 Zea mays
BX7
-
 Zea mays
TRIBOA-OMT
-
 Zea mays

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Zea mays

General Information

General Information Comment Organism
metabolism Bx7 catalyzes the last step in benzoxazinoid biosynthesis of maize Zea mays
physiological function Bx7 is required for biosynthesis of the benzoxazinoid pesticide 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, i.e. DIMBOA, that is synthesized in seedlings and stored as glucoside in maize Zea mays