Literature summary for 2.1.1.241 extracted from

  • Jonczyk, R.; Schmidt, H.; Osterrieder, A.; Fiesselmann, A.; Schullehner, K.; Haslbeck, M.; Sicker, D.; Hofmann, D.; Yalpani, N.; Simmons, C.; Frey, M.; Gier, A.
    Elucidation of the final reactions of DIMBOA-glucoside biosynthesis in maize: Characterization of Bx6 and Bx7 (2008), Plant Physiol., 146, 1053-1063.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, Bx7 maps to the cluster of Bx genes on the short arm of chromosome 4, Bx7 is located 9 cM distal to the marker adh2 at position 47, phylogenetic analysis, expression as His-tagged enzyme in Escherichia coli Zea mays

Inhibitors

Inhibitors Comment Organism Structure
additional information 1 mM Mg2+, 1 mM EDTA, 5% ethanol, or 6% DMSO have no effect on BX7 activity Zea mays

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information steady-state kinetic constants of BX7 Zea mays

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ is not required for catalysis Zea mays

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
x * 40000, native enzyme, SDS-PAGE Zea mays

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + (2R)-4,7-dihydroxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside Zea mays
-
S-adenosyl-L-homocysteine + (2R)-4-hydroxy-7-methoxy-3-oxo-3,4-dihydro-2H-1,4-benzoxazin-2-yl beta-D-glucopyranoside
-
?

Organism

Organism UniProt Comment Textmining
Zea mays
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Zea mays

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.

Subunits

Subunits Comment Organism

Synonyms

Synonyms Comment Organism

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Zea mays

General Information

General Information Comment Organism
physiological function Bx7 is required for biosynthesis of the benzoxazinoid pesticide 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3(4H)-one, i.e. DIMBOA, that is synthesized in seedlings and stored as glucoside in maize Zea mays
metabolism Bx7 catalyzes the last step in benzoxazinoid biosynthesis of maize Zea mays