Cloned (Comment) | Organism |
---|---|
recombinant expression in Escherichia coli strains BL21(DE3) and P1, coexpression with genes encoding Saccharothrix espanaensis TAL, Nicotiana tabacum CCL, and Vitis vinifera STS | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a biological platform to produce pterostilbene with the ROMT gene of Arabidopsis thaliana. Pterostilbene can be synthesized from intracellular L-tyrosine, which requires the activities of four enzymes: tyrosine ammonia lyase (TAL) from Saccharothrix espanaensis, p-coumarate:CoA ligase (CCL) from Nicotiana tabacum, stilbene synthase (STS) from Vitis vinifera, and resveratrol O-methyltransferase (ROMT). For the efficient production of pterostilbene in Escherichia coli, an engineered Escherichia coli strain P1 is used to increase the intracellular pool of L-tyrosine, which is the initial precursor of pterostilbene, with L-mehionine containing media to increase the intracellular pool of S-adenosyl-L-methionine (SAM). Pterostilbene production as high as 33.6 mg/l is achieved, which is about 3.6fold higher compared with that in the parental Escherichia coli strain harboring a plasmid for pterostilbene biosynthesis. Method, overview | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0449 | - |
trans-resveratrol | recombinant enzyme, pH 7.8, 37°C | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + trans-resveratrol | Arabidopsis thaliana | - |
2 S-adenosyl-L-homocysteine + pterostilbene | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + trans-resveratrol | - |
Arabidopsis thaliana | 2 S-adenosyl-L-homocysteine + pterostilbene | - |
? | |
additional information | mass spectrometric product analysis | Arabidopsis thaliana | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
resveratrol O-methyltransferase | - |
Arabidopsis thaliana |
ROMT | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0128 | - |
trans-resveratrol | recombinant enzyme, pH 7.8, 37°C | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.8 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | the multifunctional caffeic acid O-methyltransferase (COMT, EC 2.1.1.46) originating from Arabidopsis thaliana also catalyzes the transfer of a methyl group to resveratrol resulting in pterostilbene production (EC 2.1.1.240) | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.285 | - |
trans-resveratrol | recombinant enzyme, pH 7.8, 37°C | Arabidopsis thaliana |