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Literature summary for 2.1.1.238 extracted from

  • Li, S.; Anzai, Y.; Kinoshita, K.; Kato, F.; Sherman, D.H.
    Functional analysis of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin macrolide antibiotics (2009), Chembiochem, 10, 1297-1301.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.238

Application

Application Comment Organism
medicine the antibacterial activities of some mycinamicin products against Staphylococcus aureus are higher than those of clinical macrolide antibiotics erythromycin and leucomycin Micromonospora griseorubida

Cloned(Commentary)

Cloned (Comment) Organism
MycE is cloned into pET28b for overexpression in Escherichia coli BL21 (DE3) Micromonospora griseorubida

Inhibitors

Inhibitors Comment Organism Structure
EDTA significantly lowers the activity of MycE even in the presence of 10 mM Mg2+ Micromonospora griseorubida

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0264
-
 mycinamicin VI pH 7.4, 30°C Micromonospora griseorubida

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ activation is about 50% of the activation obtained by Mg2+ Micromonospora griseorubida
Fe2+ activation is about 70% of the activation obtained by Mg2+ Micromonospora griseorubida
Mg2+ optimal activity in presence of 10 mM MgCl2 Micromonospora griseorubida
Mn2+ activation is about 70% of the activation obtained by Mg2+ Micromonospora griseorubida

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44800
-
 44800, SDS-PAGE Micromonospora griseorubida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + mycinamicin VI Micromonospora griseorubida the enzyme is involved in the biosynthesis of mycinamicin macrolide antibiotics S-adenosyl-L-homocysteine + mycinamicin III
-
 ?

Organism

Organism UniProt Comment Textmining
Micromonospora griseorubida
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-terminal His6-tagged protein, purified by one-step Ni-NTA agarose chromatography Micromonospora griseorubida

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + mycinamicin VI the enzyme is involved in the biosynthesis of mycinamicin macrolide antibiotics Micromonospora griseorubida S-adenosyl-L-homocysteine + mycinamicin III
-
 ?
S-adenosyl-L-methionine + mycinamicin VI methylation of the C2''-OH of 6-deoxyallose. MycE possesses high substrate specificity. MycE is incapable of double methylation to generate mycinamicin IV Micromonospora griseorubida S-adenosyl-L-homocysteine + mycinamicin III
-
 ?

Subunits

Subunits Comment Organism
? 44800, SDS-PAGE Micromonospora griseorubida

Synonyms

Synonyms Comment Organism
MycE
-
 Micromonospora griseorubida

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Micromonospora griseorubida

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.083
-
 mycinamicin VI pH 7.4, 30°C Micromonospora griseorubida

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Micromonospora griseorubida

General Information

General Information Comment Organism
physiological function the enzyme is involved in the biosynthesis of mycinamicin macrolide antibiotics. Mycinamicins represent a family of macrolide antibiotics with more than twenty members produced by the rare actinomycete Micromonospora griseorubida Micromonospora griseorubida

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.14
-
 mycinamicin VI pH 7.4, 30°C Micromonospora griseorubida