Literature summary for 2.1.1.234 extracted from

Chen, H.; Yamase, H.; Murakami, K.; Chang, C.W.; Zhao, L.; Zhao, Z.; Liu, H.W.
Expression, purification, and characterization of two N,N-dimethyltransferases, tylM1 and desVI, involved in the biosynthesis of mycaminose and desosamine (2002), Biochemistry, 41, 9165-9183.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli	Streptomyces venezuelae

General Stability

General Stability	Organism
inclusion of 0.1 mM S-adenosyl-L-methionine, 0.1 mM dithiothreitol, and 10% glycerol in in buffers is crucial to prevent TylM1 from precipitating during the purification	Streptomyces venezuelae

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.2766	-	dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose	pH 7.5, 24°C, cosubstrate: 3.0 mM S-adenosyl-L-methionine	Streptomyces venezuelae
0.3074	-	dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose	pH 7.5, 24°C, cosubstrate: 3.0 mM S-adenosyl-L-methionine	Streptomyces venezuelae

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
28000	-	2 * 28000, SDS-PAGE	Streptomyces venezuelae
47400	-	gel filtration	Streptomyces venezuelae

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 S-adenosyl-L-methionine + dTDP-3-amino-4,6-dideoxy-alpha-D-glucopyranose	Streptomyces venezuelae	the enzyme is involved in the biosynthesis of desosamine	2 S-adenosyl-L-homocysteine + dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose	-	?

Organism

Organism	UniProt	Comment	Textmining
Streptomyces venezuelae	Q9ZGH6	-	-

Purification (Commentary)

Purification (Comment)	Organism
-	Streptomyces venezuelae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 S-adenosyl-L-methionine + dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose	the kcat/KM for dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose is about 20fold lower than kcat/Km for the natural substrated TDP-3-amino-4,6-dideoxy-alpha-D-glucopyranose	Streptomyces venezuelae	2 S-adenosyl-L-homocysteine + dTDP-3-dimethylamino-3,6-dideoxy-alpha-D-glucopyranose	-	?
2 S-adenosyl-L-methionine + dTDP-3-amino-4,6-dideoxy-alpha-D-glucopyranose	the enzyme is involved in the biosynthesis of desosamine	Streptomyces venezuelae	2 S-adenosyl-L-homocysteine + dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose	-	?
2 S-adenosyl-L-methionine + TDP-3-amino-4,6-dideoxy-alpha-D-glucopyranose	i.e. TDP-alpha-D-desosamine. When the reaction is quenched at an earlier time, the monomethylated species can also be detected	Streptomyces venezuelae	2 S-adenosyl-L-homocysteine + dTDP-3-dimethylamino-4,6-dideoxy-alpha-D-glucopyranose	-	?

Subunits

Subunits	Comment	Organism
homodimer	2 * 28000, SDS-PAGE	Streptomyces venezuelae
homodimer	2 * 28110, calculated from sequence	Streptomyces venezuelae

Synonyms

Synonyms	Comment	Organism
DesVI	-	Streptomyces venezuelae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
24	-	assay at	Streptomyces venezuelae

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.07	-	dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose	pH 7.5, 24°C, cosubstrate: 3.0 mM S-adenosyl-L-methionine	Streptomyces venezuelae
1.533	-	dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose	pH 7.5, 24°C, cosubstrate: 3.0 mM S-adenosyl-L-methionine	Streptomyces venezuelae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Streptomyces venezuelae

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Streptomyces venezuelae

General Information

General Information	Comment	Organism
physiological function	the enzyme catalyzes the N,N-dimethylation step in the biosynthesis of desosamine	Streptomyces venezuelae

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.25	-	dTDP-3-amino-3,6-dideoxy-alpha-D-glucopyranose	pH 7.5, 24°C, cosubstrate: 3.0 mM S-adenosyl-L-methionine	Streptomyces venezuelae
5	-	dTDP-3-amino-3,4,6-trideoxy-alpha-D-glucose	pH 7.5, 24°C, cosubstrate: 3.0 mM S-adenosyl-L-methionine	Streptomyces venezuelae

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Release 2023.1 (January 2023)