Cloned (Comment) | Organism |
---|---|
gene lcmt1, DNA and amino acid sequence determination and analysis, quantitative expression analysis in wild-type and mutant mice | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | generation of hypomorphic Lcmt1 mice with reduced protein phosphatase 2A methyltransferase expression and defects in insulin signaling, circumventing embryonic lethality with Lcmt1 deficiency | Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE | Mus musculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Mus musculus | enzyme LCMT1 is a class 1 S-adenosylmethionine-dependent methyltransferase with PP2A as its only known substrate | ? | - |
? | |
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309 | Mus musculus | - |
S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | highest expression level | Mus musculus | - |
heart | - |
Mus musculus | - |
kidney | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
muscle | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme LCMT1 is a class 1 S-adenosylmethionine-dependent methyltransferase with PP2A as its only known substrate | Mus musculus | ? | - |
? | |
additional information | peptides mimicking the C-terminal tail of PP2A are not substrates of LCMT1 | Mus musculus | ? | - |
? | |
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309 | - |
Mus musculus | S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester | - |
? | |
S-adenosyl-L-methionine + [phosphatase 2A protein]-leucine309 | specific for | Mus musculus | S-adenosyl-L-homocysteine + [phosphatase 2A protein]-leucine309 methyl ester | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
LCMT1 | - |
Mus musculus |
leucine carboxyl methyltransferase 1 | - |
Mus musculus |
PP2A methyltransferase | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | dependent on | Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | hypomorphic Lcmt1 mice show reduced protein phosphatase 2A methyltransferase expression due to splicing around the insertion, Lcmt1 transcript and LCMT1 protein levels are reduced but not eliminated. LCMT1 activity and methylation of protein phosphatase 2A are reduced in a coordinate fashion, suggesting that LCMT1 is the only PP2A methyltransferase. The mice exhibit an insulin-resistance phenotype. Knockout of Lcmt1 in mice is lethal during embryonic development. Male Lcmt1-/- animals display increased glucose-stimulated insulin secretion, increased insulin resistance, and decreased methylation of phosphatase 2A protein | Mus musculus |
physiological function | protein phosphatase 2A is the major serine/threonine phosphatase in eukaryotic cells, its assembly is governed by a variety of mechanisms, one of which is carboxyl-terminal methylation of the catalytic subunit by the leucine carboxyl methyltransferase LCMT1. Protein phosphatase 2A is nearly stoichiometrically methylated in the cytosol. Role for this methyltransferase in signaling in insulin-sensitive tissues | Mus musculus |