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Literature summary for 2.1.1.231 extracted from

  • Kim, D.H.; Kim, B.G.; Lee, Y.; Ryu, J.Y.; Lim, Y.; Hur, H.G.; Ahn, J.H.
    Regiospecific methylation of naringenin to ponciretin by soybean O-methyltransferase expressed in Escherichia coli (2005), J. Biotechnol., 119, 155-162.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as a glutathione S-transferase fusion protein. Escherichia coli harboring SOMT-2 is grown with daidzein, geninstein, apigenin, naringenin, and quercetin, respectively, and reaction products were analyzed with thin layer chromatography and HPLC Glycine max

Organism

Organism UniProt Comment Textmining
Glycine max
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-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information SOMT-2 has a regiospecific methylation activity, resulting in transforming 4'-hydroxyl group of flavonoids B-ring to 4'-methyl group. Caffeic acid, catechol, ferulic acid, and oricinol are biotransformed at less than 1% Glycine max ?
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 ?
S-adenosyl-L-methionine + apigenin 36% of the activity relative to naringenin. Escherichia coli harboring SOMT-2 is grown with daidzein, geninstein, apigenin, naringenin, and quercetin, respectively, and reaction products were analyzed with thin layer chromatography and HPLC Glycine max S-adenosyl-L-homocysteine + acacetin
-
 ?
S-adenosyl-L-methionine + daidzein 90% of the activity relative to naringenin. Escherichia coli harboring SOMT-2 is grown with daidzein, geninstein, apigenin, naringenin, and quercetin, respectively, and reaction products were analyzed with thin layer chromatography and HPLC Glycine max S-adenosyl-L-homocysteine + formononetin
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 ?
S-adenosyl-L-methionine + genistein 62% of the activity relative to naringenin. Escherichia coli harboring SOMT-2 is grown with daidzein, geninstein, apigenin, naringenin, and quercetin, respectively, and reaction products were analyzed with thin layer chromatography and HPLC Glycine max S-adenosyl-L-homocysteine + biochanin A
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 ?
S-adenosyl-L-methionine + naringenin i.e. 4',5,7-trihyroxyflavanone. Escherichia coli harboring SOMT-2 is grown with daidzein, geninstein, apigenin, naringenin, and quercetin, respectively, and reaction products were analyzed with thin layer chromatography and HPLC Glycine max S-adenosyl-L-homocysteine + ponciretin i.e. 4'-methoxy-5,7-dihydroxyflavanone ?
S-adenosyl-L-methionine + quercetin Escherichia coli harboring SOMT-2 is grown with daidzein, geninstein, apigenin, naringenin, and quercetin, respectively, and reaction products were analyzed with thin layer chromatography and HPLC Glycine max S-adenosyl-L-homocysteine + 4'-methylquercetin
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 ?

Synonyms

Synonyms Comment Organism
SOMT-2
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 Glycine max