Literature summary for 2.1.1.228 extracted from

Jin, X.; Lv, Z.; Gao, J.; Zhang, R.; Zheng, T.; Yin, P.; Li, D.; Peng, L.; Cao, X.; Qin, Y.; Persson, S.; Zheng, B.; Chen, P.
AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana (2019), Nucleic Acids Res., 47, 883-898 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene At3g56120, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21, recombinant expression in and full functional complementation of Saccgaromyces cerevisiae DELTAtrm5 mutant with the AtTRM5a gene. Yeast GBY15 mutant is defective for m1G37 nucleoside modification, and the mutant strain grows slow compared to the congenic strain GBY16	Arabidopsis thaliana

Cloned (Comment)

Organism

gene At3g56120, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21, recombinant expression in and full functional complementation of Saccgaromyces cerevisiae DELTAtrm5 mutant with the AtTRM5a gene. Yeast GBY15 mutant is defective for m1G37 nucleoside modification, and the mutant strain grows slow compared to the congenic strain GBY16

Arabidopsis thaliana

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of T-DNA insertion attrm5a deletion mutants, phenotypes, overview	Arabidopsis thaliana

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
nucleus	-	Arabidopsis thaliana	5634	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + guanine37 in tRNA	Arabidopsis thaliana	-	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?
S-adenosyl-L-methionine + guanine37 in tRNAAsp(GUC)	Arabidopsis thaliana	enzyme AtTrm5a can methylate Saccharomyces cerevisiae tRNAAsp(GUC) in vivo and in vitro	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAAsp(GUC)	-	?

Organism

Organism	UniProt	Comment	Textmining
Arabidopsis thaliana	Q93YU6	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography	Arabidopsis thaliana

Source Tissue

Source Tissue	Comment	Organism	Textmining
rosette leaf	-	Arabidopsis thaliana	-
seedling	-	Arabidopsis thaliana	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanine37 in tRNA	-	Arabidopsis thaliana	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?
S-adenosyl-L-methionine + guanine37 in tRNAAsp(GUC)	enzyme AtTrm5a can methylate Saccharomyces cerevisiae tRNAAsp(GUC) in vivo and in vitro	Arabidopsis thaliana	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAAsp(GUC)	-	?

Synonyms

Synonyms	Comment	Organism
At3g56120	-	Arabidopsis thaliana
AtTrm5a	-	Arabidopsis thaliana
tRNA (guanine(37)-N1)-methyltransferase 1	-	Arabidopsis thaliana

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Arabidopsis thaliana

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	8	assay at	Arabidopsis thaliana

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	the AdoMet binding pocket is located between E206, R209, N380, P382, Y198 and R165. Due to the flexibility of D1 domain, a structure model is projected using only D2 and D3 domains, using structure PDB 2YX1 of chain A of Trm5 from Methanococcus jannashii as template	Arabidopsis thaliana

General Information

General Information	Comment	Organism
malfunction	Arabidopsis attrm5a mutants are dwarfed and have short filaments, which leads to reduced seed setting. Proteomics data indicate differences in the abundance of proteins involved in photosynthesis, ribosome biogenesis, oxidative phosphorylation and calcium signalling. Levels of phytohormone auxin and jasmonate are reduced in attrm5a mutant, as well as expression levels of genes involved in flowering, shoot apex cell fate determination, and hormone synthesis and signalling. Taken together, loss-of-function of AtTrm5a impaires m1G and m1I methylation and leads to aberrant protein translation, disturbed hormone homeostasis and developmental defects in Arabidopsis plants	Arabidopsis thaliana
physiological function	modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m1G) on position 37, a function mediated by Trm5 homologues. Enzyme AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana. The importance of m1G37 is highlighted by its direct function in the decoding process	Arabidopsis thaliana