BRENDA - Enzyme Database
show all sequences of 2.1.1.228

AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana

Jin, X.; Lv, Z.; Gao, J.; Zhang, R.; Zheng, T.; Yin, P.; Li, D.; Peng, L.; Cao, X.; Qin, Y.; Persson, S.; Zheng, B.; Chen, P.; Nucleic Acids Res. 47, 883-898 (2019) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene At3g56120, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21, recombinant expression in and full functional complementation of Saccgaromyces cerevisiae DELTAtrm5 mutant with the AtTRM5a gene. Yeast GBY15 mutant is defective for m1G37 nucleoside modification, and the mutant strain grows slow compared to the congenic strain GBY16
Arabidopsis thaliana
Protein Variants
Protein Variants
Commentary
Organism
additional information
generation of T-DNA insertion attrm5a deletion mutants, phenotypes, overview
Arabidopsis thaliana
Localization
Localization
Commentary
Organism
GeneOntology No.
Textmining
nucleus
-
Arabidopsis thaliana
5634
-
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + guanine37 in tRNA
Arabidopsis thaliana
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNAAsp(GUC)
Arabidopsis thaliana
enzyme AtTrm5a can methylate Saccharomyces cerevisiae tRNAAsp(GUC) in vivo and in vitro
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAAsp(GUC)
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Arabidopsis thaliana
Q93YU6
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography
Arabidopsis thaliana
Source Tissue
Source Tissue
Commentary
Organism
Textmining
rosette leaf
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
S-adenosyl-L-methionine + guanine37 in tRNA
-
757863
Arabidopsis thaliana
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNAAsp(GUC)
enzyme AtTrm5a can methylate Saccharomyces cerevisiae tRNAAsp(GUC) in vivo and in vitro
757863
Arabidopsis thaliana
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAAsp(GUC)
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
At3g56120
-
Arabidopsis thaliana
AtTrm5a
-
Arabidopsis thaliana
tRNA (guanine(37)-N1)-methyltransferase 1
-
Arabidopsis thaliana
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Arabidopsis thaliana
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
assay at
Arabidopsis thaliana
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
the AdoMet binding pocket is located between E206, R209, N380, P382, Y198 and R165. Due to the flexibility of D1 domain, a structure model is projected using only D2 and D3 domains, using structure PDB 2YX1 of chain A of Trm5 from Methanococcus jannashii as template
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
Commentary
Organism
gene At3g56120, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21, recombinant expression in and full functional complementation of Saccgaromyces cerevisiae DELTAtrm5 mutant with the AtTRM5a gene. Yeast GBY15 mutant is defective for m1G37 nucleoside modification, and the mutant strain grows slow compared to the congenic strain GBY16
Arabidopsis thaliana
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
the AdoMet binding pocket is located between E206, R209, N380, P382, Y198 and R165. Due to the flexibility of D1 domain, a structure model is projected using only D2 and D3 domains, using structure PDB 2YX1 of chain A of Trm5 from Methanococcus jannashii as template
Arabidopsis thaliana
Engineering (protein specific)
Protein Variants
Commentary
Organism
additional information
generation of T-DNA insertion attrm5a deletion mutants, phenotypes, overview
Arabidopsis thaliana
Localization (protein specific)
Localization
Commentary
Organism
GeneOntology No.
Textmining
nucleus
-
Arabidopsis thaliana
5634
-
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + guanine37 in tRNA
Arabidopsis thaliana
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNAAsp(GUC)
Arabidopsis thaliana
enzyme AtTrm5a can methylate Saccharomyces cerevisiae tRNAAsp(GUC) in vivo and in vitro
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAAsp(GUC)
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant GST-tagged enzyme from Escherichia coli strain BL21 by glutathione affinity chromatography
Arabidopsis thaliana
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
rosette leaf
-
Arabidopsis thaliana
-
seedling
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
S-adenosyl-L-methionine + guanine37 in tRNA
-
757863
Arabidopsis thaliana
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNAAsp(GUC)
enzyme AtTrm5a can methylate Saccharomyces cerevisiae tRNAAsp(GUC) in vivo and in vitro
757863
Arabidopsis thaliana
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNAAsp(GUC)
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Arabidopsis thaliana
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
8
assay at
Arabidopsis thaliana
General Information
General Information
Commentary
Organism
malfunction
Arabidopsis attrm5a mutants are dwarfed and have short filaments, which leads to reduced seed setting. Proteomics data indicate differences in the abundance of proteins involved in photosynthesis, ribosome biogenesis, oxidative phosphorylation and calcium signalling. Levels of phytohormone auxin and jasmonate are reduced in attrm5a mutant, as well as expression levels of genes involved in flowering, shoot apex cell fate determination, and hormone synthesis and signalling. Taken together, loss-of-function of AtTrm5a impaires m1G and m1I methylation and leads to aberrant protein translation, disturbed hormone homeostasis and developmental defects in Arabidopsis plants
Arabidopsis thaliana
physiological function
modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m1G) on position 37, a function mediated by Trm5 homologues. Enzyme AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana. The importance of m1G37 is highlighted by its direct function in the decoding process
Arabidopsis thaliana
General Information (protein specific)
General Information
Commentary
Organism
malfunction
Arabidopsis attrm5a mutants are dwarfed and have short filaments, which leads to reduced seed setting. Proteomics data indicate differences in the abundance of proteins involved in photosynthesis, ribosome biogenesis, oxidative phosphorylation and calcium signalling. Levels of phytohormone auxin and jasmonate are reduced in attrm5a mutant, as well as expression levels of genes involved in flowering, shoot apex cell fate determination, and hormone synthesis and signalling. Taken together, loss-of-function of AtTrm5a impaires m1G and m1I methylation and leads to aberrant protein translation, disturbed hormone homeostasis and developmental defects in Arabidopsis plants
Arabidopsis thaliana
physiological function
modified nucleosides on tRNA are critical for decoding processes and protein translation. tRNAs can be modified through 1-methylguanosine (m1G) on position 37, a function mediated by Trm5 homologues. Enzyme AtTrm5a catalyses 1-methylguanosine and 1-methylinosine formation on tRNAs and is important for vegetative and reproductive growth in Arabidopsis thaliana. The importance of m1G37 is highlighted by its direct function in the decoding process
Arabidopsis thaliana
Other publictions for EC 2.1.1.228
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Protein Variants
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
kcat/KM [mM/s]
kcat/KM [mM/s] (protein specific)
755697
Zhong
Targeting the bacterial epitr ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
ACS Infect. Dis.
5
326-335
2019
-
1
1
-
-
-
63
2
-
-
-
2
-
8
-
-
-
-
-
-
-
-
6
-
3
-
-
1
2
-
-
-
1
-
-
63
-
1
1
1
-
-
-
63
63
-
2
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
1
2
-
-
-
-
-
1
1
-
2
2
756152
Li
Backbone resonance assignment ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
Biomol. NMR Assign.
13
327-332
2019
-
1
1
-
-
-
1
-
-
-
-
2
-
7
-
-
1
-
-
1
-
-
2
2
2
-
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
1
-
1
-
-
2
2
-
-
-
-
1
-
-
-
-
2
2
-
-
-
756153
Li
Backbone resonance assignment ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
Biomol. NMR Assign.
13
49-53
2019
-
-
1
-
-
-
2
-
-
-
-
2
-
6
-
-
1
-
-
-
-
-
2
-
2
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
2
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
756774
Hou
Codon-specific translation by ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720, Saccharomyces cerevisiae ATCC 204508
Front. Genet.
10
713
2019
-
-
-
-
1
-
-
-
-
2
-
7
-
8
-
-
-
-
-
-
-
-
7
-
4
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
1
8
8
1
-
-
757408
Whitehouse
Development of inhibitors aga ...
Mycobacteroides abscessus
J. Med. Chem.
62
7210-7232
2019
-
1
1
1
-
-
50
-
-
-
-
1
-
6
-
-
1
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
1
-
-
-
50
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
757409
Zhong
Thienopyrimidinone derivative ...
Staphylococcus aureus, Mycobacterium tuberculosis, Pseudomonas aeruginosa
J. Med. Chem.
62
7788-7805
2019
-
-
3
2
-
3
42
3
-
2
-
3
-
5
-
-
-
-
-
-
-
-
3
-
12
1
-
-
-
1
-
-
3
-
-
26
-
-
3
3
2
-
3
26
42
-
3
-
2
-
3
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
757863
Jin
AtTrm5a catalyses 1-methylgua ...
Arabidopsis thaliana
Nucleic Acids Res.
47
883-898
2019
-
-
1
-
1
-
-
-
1
1
-
2
-
8
-
-
1
-
-
2
-
-
2
-
3
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
2
-
-
-
1
-
2
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
758329
Jaroensuk
Crystal structure and catalyt ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
RNA
25
1481-1496
2019
-
1
1
1
1
-
1
3
-
2
-
2
-
10
-
-
1
-
-
-
-
-
14
2
5
1
-
-
2
1
-
-
1
3
-
-
-
1
1
1
1
1
-
-
1
3
3
-
2
-
2
-
-
-
1
-
-
-
-
14
2
1
-
-
2
1
-
-
-
-
2
2
-
2
2
757201
Zhou
A hypertension-associated mit ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii NBRC 100440, Methanocaldococcus jannaschii DSM 2661, Methanocaldococcus jannaschii ATCC 43067, Methanocaldococcus jannaschii JAL-1, Methanocaldococcus jannaschii JCM 10045
J. Biol. Chem.
293
1425-1438
2018
-
-
-
-
1
-
-
-
-
-
-
6
-
17
-
-
-
-
-
-
-
-
12
-
5
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
755960
Wu
The crystal structure of the ...
Pyrococcus abyssi
Biochem. Biophys. Res. Commun.
493
240-245
2017
-
-
1
1
1
-
-
-
-
-
-
1
-
9
-
-
1
-
-
-
-
-
2
1
4
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
4
4
-
-
-
756161
Goto-Ito
Trm5 and TrmD two enzymes fr ...
Escherichia coli, Haemophilus influenzae, Methanocaldococcus jannaschii, Pyrococcus abyssi, Pyrococcus abyssi Orsay, Methanocaldococcus jannaschii NBRC 100440, Haemophilus influenzae RD, Methanocaldococcus jannaschii DSM 2661, Methanocaldococcus jannaschii ATCC 43067, Methanocaldococcus jannaschii JAL-1, Haemophilus influenzae DSM 11121, Haemophilus influenzae KW20, Haemophilus influenzae ATCC 51907, Methanocaldococcus jannaschii JCM 10045
Biomolecules
7
32
2017
-
-
-
1
-
-
-
4
-
-
-
16
-
36
-
-
-
-
-
-
-
-
30
4
10
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
1
-
-
-
-
-
4
-
-
-
16
-
-
-
-
-
-
-
-
30
4
-
-
-
-
-
-
-
-
-
14
14
-
-
-
756163
Hori
Transfer RNA methyltransferas ...
Haemophilus influenzae, Aquifex aeolicus, Escherichia coli
Biomolecules
7
E23
2017
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
6
3
3
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
6
3
-
-
-
-
-
-
-
-
-
7
7
-
-
-
756631
Hou
TrmD A methyl transferase fo ...
Haemophilus influenzae, Aquifex aeolicus, Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
Enzymes
41
89-115
2017
-
4
4
-
-
-
-
-
-
16
-
12
-
8
-
-
-
-
-
-
-
-
12
8
6
-
-
-
-
-
-
-
4
-
-
-
-
4
4
4
-
-
-
-
-
-
-
-
16
-
12
-
-
-
-
-
-
-
-
12
8
-
-
-
-
-
-
-
-
-
16
16
-
-
-
758348
Wang
Structural insight into the m ...
Methanocaldococcus jannaschii, Pyrococcus abyssi, Methanocaldococcus jannaschii DSM 2661
Sci. Adv.
3
e1700195
2017
-
-
1
2
7
-
-
-
-
2
-
4
-
6
-
-
1
-
-
-
-
-
7
1
6
2
-
-
-
2
-
-
2
-
-
-
-
-
1
2
2
7
-
-
-
-
-
-
2
-
4
-
-
-
1
-
-
-
-
7
1
2
-
-
-
2
-
-
-
-
4
4
-
-
-
757790
Christian
Methyl transfer by substrate ...
Haemophilus influenzae
Nat. Struct. Mol. Biol.
23
941-948
2016
-
1
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
4
4
-
-
-
758324
Urbonavicius
Evolution of tRNAPhe imG2 met ...
Nanoarchaeum equitans, Pyrococcus abyssi, Pyrococcus abyssi Orsay
RNA
22
1871-1883
2016
-
-
2
-
7
-
-
-
-
2
-
11
-
4
-
-
2
-
-
-
-
-
19
-
14
2
-
-
-
2
-
-
2
-
-
-
-
-
2
2
-
7
-
-
-
-
-
-
2
-
11
-
-
-
2
-
-
-
-
19
-
2
-
-
-
2
-
-
-
-
8
8
-
-
-
758376
Wang
Crystal structures of the bif ...
Pyrococcus abyssi
Sci. Rep.
6
33553
2016
-
-
1
1
2
-
-
-
-
1
-
2
-
9
-
-
1
-
-
-
-
-
3
2
5
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
2
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
4
4
-
-
-
735421
Powell
TRMT5 mutations cause a defect ...
Homo sapiens
Am. J. Hum. Genet.
97
319-328
2015
-
-
1
-
3
-
-
-
1
-
-
1
-
7
-
-
-
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
3
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
4
4
-
-
-
737174
Ito
Structural basis for methyl-do ...
Haemophilus influenzae, Thermotoga maritima, Haemophilus influenzae DSM 11121
Proc. Natl. Acad. Sci. USA
112
E4197-E4205
2015
-
-
-
2
-
-
-
12
-
-
-
3
-
4
-
-
-
2
-
-
-
-
17
2
2
-
-
-
10
1
-
-
2
-
-
-
-
-
-
2
2
-
-
-
-
-
12
-
-
-
3
-
-
-
-
-
-
-
-
17
2
-
-
-
10
1
-
-
-
-
4
4
-
10
10
757582
Hou
Kinetic analysis of tRNA meth ...
Escherichia coli, Haemophilus influenzae, Homo sapiens, Methanocaldococcus jannaschii, Methanocaldococcus jannaschii NBRC 100440, Haemophilus influenzae RD, Methanocaldococcus jannaschii DSM 2661, Methanocaldococcus jannaschii ATCC 43067, Methanocaldococcus jannaschii JAL-1, Haemophilus influenzae DSM 11121, Haemophilus influenzae KW20, Haemophilus influenzae ATCC 51907, Methanocaldococcus jannaschii JCM 10045
Methods Enzymol.
560
91-116
2015
-
-
-
-
-
-
-
4
-
4
-
13
-
28
-
-
-
-
-
-
-
-
26
-
13
4
-
-
-
4
-
-
4
-
-
-
-
-
-
4
-
-
-
-
-
-
4
-
4
-
13
-
-
-
-
-
-
-
-
26
-
4
-
-
-
4
-
-
-
-
12
12
-
-
-
729858
Kawamura
Transfer RNA methyltransferase ...
Thermoplasma acidophilum, Thermoplasma acidophilum HO-62
Int. J. Mol. Sci.
16
91-113
2014
-
-
1
-
-
-
-
-
-
-
-
-
-
9
-
-
1
-
-
-
-
-
1
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
735927
Sakaguchi
A divalent metal ion-dependent ...
Escherichia coli
Chem. Biol.
21
1351-1360
2014
-
1
-
-
-
-
-
1
-
4
-
1
-
1
-
-
-
1
-
-
-
-
2
1
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
-
1
-
4
-
1
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
736428
Masuda
The temperature sensitivity of ...
Escherichia coli
J. Biol. Chem.
288
28987-28996
2013
-
-
1
-
1
-
-
7
-
1
-
1
-
3
-
-
1
-
-
-
-
-
3
1
2
1
-
-
6
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
7
-
1
-
1
-
-
-
1
-
-
-
-
3
1
1
-
-
6
1
-
-
-
-
2
2
-
6
6
737226
Christian
Conservation of structure and ...
Homo sapiens
RNA
19
1192-1199
2013
-
-
1
-
9
-
-
3
-
-
-
1
-
12
-
-
1
1
-
-
-
-
2
-
1
1
-
-
1
1
1
-
1
-
-
-
-
-
1
1
-
9
-
-
-
-
3
-
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
1
1
1
-
-
-
2
2
-
1
1
737228
Paris
The T. brucei TRM5 methyltrans ...
Trypanosoma brucei brucei, Trametes pubescens 927 / 4 GUTat10.1 / TREU927
RNA
19
649-658
2013
-
-
1
-
1
-
-
-
2
-
-
2
-
5
-
-
1
-
-
-
-
-
12
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
2
-
-
2
-
-
-
1
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
721035
Sakaguchi
Recognition of guanosine by di ...
Escherichia coli, Methanocaldococcus jannaschii
RNA
18
1687-1701
2012
-
-
-
-
-
-
-
2
-
-
-
2
-
2
-
-
-
2
-
-
-
-
10
-
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
2
-
-
-
-
-
-
-
-
10
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
721029
Lahoud
Differentiating analogous tRNA ...
Escherichia coli, Methanocaldococcus jannaschii
RNA
17
1236-1246
2011
-
-
-
-
-
-
24
2
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
2
2
-
-
-
-
-
-
2
24
-
-
-
-
-
2
-
-
-
-
24
24
2
-
-
-
2
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
720240
Christian
Control of catalytic cycle by ...
Escherichia coli, Methanocaldococcus jannaschii
J. Mol. Biol.
400
204-217
2010
-
-
-
-
-
-
-
2
-
-
-
4
-
2
-
-
-
-
-
-
-
-
6
2
2
-
-
-
3
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
4
-
-
-
-
-
-
-
-
6
2
-
-
-
3
-
-
-
-
-
-
-
-
-
-
721026
Christian
Mechanism of N-methylation by ...
Methanocaldococcus jannaschii
RNA
16
2484-2492
2010
-
-
-
1
18
-
-
1
-
-
-
1
-
1
-
-
-
1
-
1
-
-
3
-
2
1
-
-
-
-
1
-
1
-
-
-
-
-
-
1
1
18
-
-
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
3
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
701123
Goto-Ito
Crystal structure of archaeal ...
Methanocaldococcus jannaschii
Proteins
72
1274-1289
2008
-
-
-
1
-
-
1
1
-
-
-
-
-
11
-
-
-
-
-
-
-
-
1
-
2
1
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
1
1
-
-
-
-
-
-
-
1
1
712103
Toyooka
Stabilization of tRNA (mG37) m ...
Aquifex aeolicus
Genes Cells
13
807-816
2008
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
687609
Lee
Yeast mitochondrial initiator ...
Saccharomyces cerevisiae
J. Biol. Chem.
282
27744-27753
2007
-
-
1
-
-
-
-
-
2
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
1
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
1
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
712739
Christian
Distinct determinants of tRNA ...
Escherichia coli, Methanocaldococcus jannaschii
J. Mol. Biol.
373
623-632
2007
-
-
-
-
-
-
-
5
-
-
-
2
-
2
-
-
-
-
-
-
-
-
5
-
2
2
-
-
5
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
5
-
-
-
2
-
-
-
-
-
-
-
-
5
-
2
-
-
5
2
-
-
-
-
2
2
-
5
5
673850
Takeda
The substrate specificity of t ...
Aquifex aeolicus
Genes Cells
11
1353-1365
2006
-
-
1
-
-
-
-
13
-
-
-
1
-
6
-
-
1
-
-
-
-
-
15
-
3
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
13
-
-
-
1
-
-
-
1
-
-
-
-
15
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
711197
Christian
Catalysis by the second class ...
Methanocaldococcus jannaschii
Biochemistry
45
7463-7473
2006
-
-
-
-
9
-
-
11
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
2
1
-
-
11
1
-
-
-
-
-
-
-
-
-
-
-
9
-
-
-
-
11
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
11
1
-
-
-
-
1
1
-
11
11
661154
Brule
Isolation and characterization ...
Escherichia coli, Homo sapiens
Biochemistry
43
9243-9255
2004
1
-
2
-
-
-
5
13
-
3
2
2
-
9
-
-
2
-
-
-
-
-
20
2
4
2
-
-
-
4
2
-
-
-
-
-
1
-
2
-
-
-
-
-
5
-
13
-
3
2
2
-
-
-
2
-
-
-
-
20
2
2
-
-
-
4
2
-
-
-
-
-
-
-
-
662010
O'Dwyer
Characterization of Streptococ ...
Streptococcus pneumoniae
J. Bacteriol.
186
2346-2354
2004
-
-
1
-
-
-
-
2
-
4
3
1
-
5
-
-
1
-
-
-
-
-
3
1
1
1
-
-
2
2
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
2
-
4
3
1
-
-
-
1
-
-
-
-
3
1
1
-
-
2
2
1
-
-
-
-
-
-
2
2
662622
Christian
Distinct origins of tRNA(m1G37 ...
Methanocaldococcus jannaschii
J. Mol. Biol.
339
707-719
2004
-
1
1
-
5
-
-
-
-
-
1
-
-
6
-
-
1
-
-
-
-
-
7
1
3
1
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
5
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
7
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
661585
Ahn
Crystal structure of tRNA(m1G3 ...
Haemophilus influenzae
EMBO J.
22
2593-2603
2003
-
-
-
1
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
712737
Elkins
Insights into catalysis by a k ...
Escherichia coli
J. Mol. Biol.
333
931-949
2003
-
-
1
1
44
-
-
-
-
-
2
-
-
3
-
-
1
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
44
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
713482
Liu
Crystal structure of tRNA (m1G ...
Aquifex aeolicus
Proteins
53
326-328
2003
-
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
1
-
-
-
710708
Kim
Crystallization and preliminar ...
Haemophilus influenzae
Acta Crystallogr. Sect. D
59
183-184
2002
-
-
1
1
-
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-