Cloned (Comment) | Organism |
---|---|
gene trmD, sequence comparisons | Staphylococcus aureus |
gene trmD, sequence comparisons | Pseudomonas aeruginosa |
gene trmD, sequence comparisons | Mycobacterium tuberculosis |
Crystallization (Comment) | Organism |
---|---|
analysis of the crystal structure of apo-enzyme, of enzyme with bound ligand S-adenosyl-L-homocysteine (PDB IDs 5ZHI and 5ZHJ, respectively), and of enzyme with bound inhibitors N-(4-((octylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide, N-([4-[(4-aminopiperidin-1-yl)methyl]phenyl]methyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide , and N-(4-((cyclohexyl(ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide, PDB IDs 6JOF, 5ZHK, and 5ZHL, respectively, at resolutions of 1.75-2.76 A. Sitting drop vapor diffusion method, mixing of 0.002 ml of protein solution with 0.001 ml of precipitant solution containing 100 mM Bis-Tris propane, pH 6.5, 20% w/v PEG3350, and 0.1 M ammonium acetate, soaking of crystals with 1-5 mM inhibitor or 5 mM SAH in their respective precipitating solution supplemented with 20% v/v glycerol, X-ray diffraction structure determination and analysis | Mycobacterium tuberculosis |
analysis of the crystal structure of enzyme with bound inhibitors N-([4-[(4-aminopiperidin-1-yl)methyl]phenyl]methyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide, N-(4-((diethylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide, and N-(4-((octylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide, PDB IDs 6JOE, 5ZHM, and 5ZHN, respectively. Hanging drop vapor diffusion method, mixing of equal volumes of 20 mg/m protein and precipitant solution containing 0.1 M Tris-HCl, pH 8.6-8.8, 20% v/v MPD, 20% w/v PEG 1000, and 5% w/v PEG200, and incubation at 20°C, soaking of crystals with 1-5 mM inhibitor in the precipitating solution supplemented with 20% v/v glycerol at 20°C for at least 4 h, X-ray diffraction structure determination and analysis | Pseudomonas aeruginosa |
General Stability | Organism |
---|---|
stabilization of the enzyme by binding of small molecules, overview | Staphylococcus aureus |
stabilization of the enzyme by binding of small molecules, overview | Pseudomonas aeruginosa |
stabilization of the enzyme by binding of small molecules, overview | Mycobacterium tuberculosis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-((4-azidobenzyl)oxy)-N-(4-((octylamino)methyl)benzyl)thieno-[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
4-(hexyloxy)-N-(4-((octylamino)methyl)benzyl)thieno[2,3-d]-pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
4-methoxy-N-(4-(morpholinomethyl)benzyl)thieno[2,3-d]-pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
4-oxo-N-(4-((pentylamino)methyl)benzyl)-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
4-oxo-N-(4-(piperidin-1-ylmethyl)benzyl)-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
additional information | synthesis of thienopyrimidinone derivatives that inhibit bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) by restructuring the active site with a tyrosine-flipping mechanism, overview. The tyrosine-flipping mechanism is uniquely found in Pseudomonas aeruginosa TrmD and renders the enzyme inaccessible to the cofactor S-adenosyl-L-methionine (SAM) and probably to the substrate tRNA | Mycobacterium tuberculosis | |
additional information | synthesis of thienopyrimidinone derivatives that inhibit bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) by restructuring the active site with a tyrosine-flipping mechanism, nanomolar potency against TrmD in vitro, overview. This tyrosine-flipping mechanism is uniquely found in Pseudomonas aeruginosa TrmD and renders the enzyme inaccessible to the cofactor S-adenosyl-L-methionine (SAM) and probably to the substrate tRNA. Biochemical structure-activity relationships (SAR) for TrmD inhibitors, the thienopyrimidinone substituent flexibility is critical for potent TrmD inhibition. Analysis of hemolytic activity of the compounds. Effect of side chain length of 15 analogues | Pseudomonas aeruginosa | |
additional information | synthesis of thienopyrimidinone derivatives that inhibit bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) by restructuring the active site with a tyrosine-flipping mechanism, overview. The tyrosine-flipping mechanism is uniquely found in Pseudomonas aeruginosa TrmD and renders the enzyme inaccessible to the cofactor S-adenosyl-L-methionine (SAM) and probably to the substrate tRNA | Staphylococcus aureus | |
N-(4-((((3s,5s,7s)-adamantan-1-yl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-(((2-(2-(2-aminoethoxy)ethoxy)ethyl)amino)methyl)-benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-(((2-(2-ethoxyethoxy)ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-(((2-(2-hydroxyethoxy)ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-(((4-aminobutyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide dihydrochloride | - |
Pseudomonas aeruginosa | |
N-(4-(((5-(1,5-dihydroxy-4-oxo-1,4-dihydropyridine-2-carboxamido)pentyl)amino) methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-(((5-aminopentyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-(((7-aminohexyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((1,5-dihydroxy-4-oxo-1,4-dihydropyridine-2-carboxamido)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]-pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((benzyl(ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((benzyl(hexyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((benzyl(octyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((benzylamino)methyl)benzyl)-4-(hexyloxy)thieno[2,3-d]-pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((benzylamino)methyl)benzyl)-4-methoxythieno[2,3-d]-pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((benzylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((benzylamino)methyl)benzyl)-4-propoxythieno[2,3-d]-pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((butylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((cyclohexyl(ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | enzyme-bound crystal structure, overview | Mycobacterium tuberculosis | |
N-(4-((cyclohexyl(ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((cyclohexylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((decylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((diethylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide | enzyme-bound crystal structure, overview | Pseudomonas aeruginosa | |
N-(4-((dodecylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((ethyl(octyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((octylamino)methyl)benzyl)-4-moxythieno[2,3-d]-pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-((octylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | enzyme-bound crystal structure, overview | Mycobacterium tuberculosis | |
N-(4-((octylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | enzyme-bound crystal structure, overview | Pseudomonas aeruginosa | |
N-(4-((octylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Staphylococcus aureus | |
N-(4-((octylamino)methyl)benzyl)-4-propoxythieno[2,3-d]-pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-(morpholinomethyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-(4-(morpholinomethyl)benzyl)-4-propoxythieno[2,3-d]-pyrimidine-5-carboxamide | - |
Pseudomonas aeruginosa | |
N-([4-[(4-aminopiperidin-1-yl)methyl]phenyl]methyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | the binding of AZ51 does not induce the side chain flip of Tyr111 in MtbTrmD, while the corresponding residue Tyr120 in PaTrmD turned 180° to form stacking interactions with the phenyl ring of the inhibitor, enzyme-bound crystal structure, overview | Mycobacterium tuberculosis | |
N-([4-[(4-aminopiperidin-1-yl)methyl]phenyl]methyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | i.e. AZ51, inhibitor binding induces conformational changes of the wall loop, whereupon the side chain of aromatic ring of Tyr120 flips about 180° and forms stacking interactions with both the phenyl and piperidine rings of AZ51. This feature appears unique to AZ51 and P | Pseudomonas aeruginosa | |
N-([4-[(4-aminopiperidin-1-yl)methyl]phenyl]methyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | - |
Staphylococcus aureus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | binding kinetics of TrmD ligands | Staphylococcus aureus | |
additional information | - |
additional information | binding kinetics of TrmD ligands | Pseudomonas aeruginosa | |
additional information | - |
additional information | binding kinetics of TrmD ligands | Mycobacterium tuberculosis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Pseudomonas aeruginosa | |
Mg2+ | required | Mycobacterium tuberculosis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA | Staphylococcus aureus | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | Pseudomonas aeruginosa | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | Mycobacterium tuberculosis | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium tuberculosis | - |
- |
- |
Pseudomonas aeruginosa | - |
- |
- |
Staphylococcus aureus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Staphylococcus aureus | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Pseudomonas aeruginosa | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Mycobacterium tuberculosis | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bacterial tRNA (guanine37-N1)-methyltransferase | - |
Staphylococcus aureus |
bacterial tRNA (guanine37-N1)-methyltransferase | - |
Pseudomonas aeruginosa |
bacterial tRNA (guanine37-N1)-methyltransferase | - |
Mycobacterium tuberculosis |
MtbTrmD | - |
Mycobacterium tuberculosis |
PaTrmD | - |
Pseudomonas aeruginosa |
SaTrmD | - |
Staphylococcus aureus |
TrmD | - |
Staphylococcus aureus |
TrmD | - |
Pseudomonas aeruginosa |
TrmD | - |
Mycobacterium tuberculosis |
tRNA (guanine37-N1)-methyltransferase | - |
Staphylococcus aureus |
tRNA (guanine37-N1)-methyltransferase | - |
Pseudomonas aeruginosa |
tRNA (guanine37-N1)-methyltransferase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Staphylococcus aureus | |
S-adenosyl-L-methionine | - |
Pseudomonas aeruginosa | |
S-adenosyl-L-methionine | - |
Mycobacterium tuberculosis |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.000024 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-((octylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.000025 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-((decylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.00008 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-(((4-aminobutyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide dihydrochloride | |
0.000085 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | 4-oxo-N-(4-((pentylamino)methyl)benzyl)-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide | |
0.00011 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-(((2-(2-hydroxyethoxy)ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.00011 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-(((5-aminopentyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.00013 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-((benzylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide | |
0.00014 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-(((7-aminohexyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.00015 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-((butylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.00018 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-([4-[(4-aminopiperidin-1-yl)methyl]phenyl]methyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.00021 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-(((2-(2-ethoxyethoxy)ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.00037 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-((ethyl(octyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.00038 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-((dodecylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide | |
0.00045 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-(((2-(2-(2-aminoethoxy)ethoxy)ethyl)amino)methyl)-benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.00049 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-((cyclohexyl(ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.0007 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-((1,5-dihydroxy-4-oxo-1,4-dihydropyridine-2-carboxamido)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]-pyrimidine-5-carboxamide | |
0.00072 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-((((3s,5s,7s)-adamantan-1-yl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.00073 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-((diethylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno-[2,3-d]pyrimidine-5-carboxamide | |
0.00079 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-((cyclohexylamino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.0011 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | 4-oxo-N-(4-(piperidin-1-ylmethyl)benzyl)-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.0013 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-(((5-(1,5-dihydroxy-4-oxo-1,4-dihydropyridine-2-carboxamido)pentyl)amino) methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.0018 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | 4-((4-azidobenzyl)oxy)-N-(4-((octylamino)methyl)benzyl)thieno-[2,3-d]pyrimidine-5-carboxamide | |
0.0022 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-((benzyl(ethyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.0022 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-((benzyl(hexyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.003 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-(morpholinomethyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide | |
0.012 | - |
pH 8.0, 37°C | Pseudomonas aeruginosa | N-(4-((benzyl(octyl)amino)methyl)benzyl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidine-5-carboxamide |
General Information | Comment | Organism |
---|---|---|
physiological function | bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) catalyzes methyl transfer from S-adenosyl-L-methionine (SAM) to the guanine N1 at nucleotide position 37 in a subset of bacterial tRNA isoacceptors and has proven to be an essential enzyme in most bacterial species | Staphylococcus aureus |
physiological function | bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) catalyzes methyl transfer from S-adenosyl-L-methionine (SAM) to the guanine N1 at nucleotide position 37 in a subset of bacterial tRNA isoacceptors and has proven to be an essential enzyme in most bacterial species | Pseudomonas aeruginosa |
physiological function | bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) catalyzes methyl transfer from S-adenosyl-L-methionine (SAM) to the guanine N1 at nucleotide position 37 in a subset of bacterial tRNA isoacceptors and has proven to be an essential enzyme in most bacterial species | Mycobacterium tuberculosis |