Literature summary for 2.1.1.228 extracted from

Li, Y.; Zhong, W.; Koay, A.Z.; Ng, H.Q.; Koh-Stenta, X.; Nah, Q.; Lim, S.H.; Larsson, A.; Lescar, J.; Hill, J.; Dedon, P.C.; Kang, C.
Backbone resonance assignment for the N-terminal region of bacterial tRNA-(N1G37) methyltransferase (2019), Biomol. NMR Assign., 13, 49-53 .

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Cloned(Commentary)

Cloned (Comment)	Organism
gene trmD, recombinant expression of His-tagged isolated N-terminal domain in Escherichia coli strain BL21(DE3)	Pseudomonas aeruginosa

Inhibitors

Inhibitors	Comment	Organism	Structure
additional information	the N-terminal domain is a useful construct to probe the molecular interactions with SAM competitive inhibitors, in the search for molecules with antibiotic activity	Pseudomonas aeruginosa
sinefungin	an active-site inhibitor and S-adenosyl-L-methionine (SAM) analogue, that binds at the N-terminal dommain in the SAM binding site. Structure analysis of crystallized isolated dimeric NTD, overview	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + guanine37 in tRNA	Pseudomonas aeruginosa	-	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?
S-adenosyl-L-methionine + guanine37 in tRNA	Pseudomonas aeruginosa UCBPP-PA14	-	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q02RL6	-	-
Pseudomonas aeruginosa UCBPP-PA14	Q02RL6	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged isolated N-terminal domain from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration	Pseudomonas aeruginosa

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanine37 in tRNA	-	Pseudomonas aeruginosa	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?
S-adenosyl-L-methionine + guanine37 in tRNA	-	Pseudomonas aeruginosa UCBPP-PA14	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?

Synonyms

Synonyms	Comment	Organism
bacterial tRNA-(N1G37) methyltransferase	-	Pseudomonas aeruginosa
TrmD	-	Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	metabolites such as S-adenosyl-L-methionine (SAM), S-adenosyl-L-homocysteine (SAH), and methylthioadenosine (MTA) enhance the thermostability of the enzyme's N-terminal domain by increasing its melting temperature (Tm)	Pseudomonas aeruginosa

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	SAM	Pseudomonas aeruginosa

General Information

General Information	Comment	Organism
additional information	the N-terminal domain (NTD) contains the S-adenosyl-L-methionine (SAM) binding region, this domain binds to SAM, S-adenosyl-L-homocysteine (SAH), and active-site inhibitors such as the SAM analogue sinefungin. Metabolites such as SAM, SAH and MTA enhance the thermostability of NTD by increasing its melting temperature (Tm), dynamics and ligand binding of NTD	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)