BRENDA - Enzyme Database
show all sequences of 2.1.1.228

Backbone resonance assignment for the full length tRNA-(N1G37) methyltransferase of Pseudomonas aeruginosa

Li, Y.; Zhong, W.; Koay, A.Z.; Ng, H.Q.; Nah, Q.; Wong, Y.H.; Hill, J.; Lescar, J.; Dedon, P.C.; Kang, C.; Biomol. NMR Assign. 13, 327-332 (2019) View publication on PubMed

Data extracted from this reference:

Application
Application
Commentary
Organism
drug development
bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) from Pseudomonas aeruginosa is a good target for development of antibacterial compounds
Pseudomonas aeruginosa
Cloned(Commentary)
Cloned (Commentary)
Organism
gene trmD, recombinant expression of His-tagged full-length enzyme in Escherichia coli strain BL21(DE3)
Pseudomonas aeruginosa
Inhibitors
Inhibitors
Commentary
Organism
Structure
sinefungin
competitive inhibtior
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + guanine37 in tRNA
Pseudomonas aeruginosa
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Pseudomonas aeruginosa UCBPP-PA14
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas aeruginosa
Q02RL6
-
-
Pseudomonas aeruginosa UCBPP-PA14
Q02RL6
-
-
Purification (Commentary)
Purification (Commentary)
Organism
recombinant His-tagged full-length enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration
Pseudomonas aeruginosa
Source Tissue
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
S-adenosyl-L-methionine + guanine37 in tRNA
-
756152
Pseudomonas aeruginosa
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
756152
Pseudomonas aeruginosa UCBPP-PA14
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
Subunits
Subunits
Commentary
Organism
dimer
enzyme TrmD comprises two domains with the N-terminal domain binding to the S-adenosyl-L-methionine (SAM) cofactor and the C-terminal domain critical for tRNA binding. Bacterial TrmD is functional as a dimer
Pseudomonas aeruginosa
More
backbone NMR resonance assignments for the full length TrmD protein of Pseudomonas aeruginosa and secondary structure analysis
Pseudomonas aeruginosa
Synonyms
Synonyms
Commentary
Organism
TrmD
-
Pseudomonas aeruginosa
tRNA-(N1G37) methyltransferase
-
Pseudomonas aeruginosa
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pseudomonas aeruginosa
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Pseudomonas aeruginosa
Application (protein specific)
Application
Commentary
Organism
drug development
bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) from Pseudomonas aeruginosa is a good target for development of antibacterial compounds
Pseudomonas aeruginosa
Cloned(Commentary) (protein specific)
Commentary
Organism
gene trmD, recombinant expression of His-tagged full-length enzyme in Escherichia coli strain BL21(DE3)
Pseudomonas aeruginosa
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Pseudomonas aeruginosa
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
sinefungin
competitive inhibtior
Pseudomonas aeruginosa
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + guanine37 in tRNA
Pseudomonas aeruginosa
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Pseudomonas aeruginosa UCBPP-PA14
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant His-tagged full-length enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, gel filtration, and ultrafiltration
Pseudomonas aeruginosa
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
S-adenosyl-L-methionine + guanine37 in tRNA
-
756152
Pseudomonas aeruginosa
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
756152
Pseudomonas aeruginosa UCBPP-PA14
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
dimer
enzyme TrmD comprises two domains with the N-terminal domain binding to the S-adenosyl-L-methionine (SAM) cofactor and the C-terminal domain critical for tRNA binding. Bacterial TrmD is functional as a dimer
Pseudomonas aeruginosa
More
backbone NMR resonance assignments for the full length TrmD protein of Pseudomonas aeruginosa and secondary structure analysis
Pseudomonas aeruginosa
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
assay at
Pseudomonas aeruginosa
General Information
General Information
Commentary
Organism
additional information
backbone NMR resonance assignments for the full length TrmD protein of Pseudomonas aeruginosa and secondary structure analysis
Pseudomonas aeruginosa
physiological function
methylation of guanine at position 37 in RNA is critical for bacterial growth, as this reaction is essential for preventing reading frame shifts during translation and therefore for maintaining the fidelity of protein synthesis. This methylation is catalyzed by the tRNA (guanine37-N1)-methyltransferase (TrmD). TrmD uses S-adenosyl-l-methionine (SAM) as a cofactor and transfers the methyl group to the N1 atom of G37 in tRNA. Bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) plays important roles in translation. and is critical for growth of Pseudomonas aeruginosa
Pseudomonas aeruginosa
General Information (protein specific)
General Information
Commentary
Organism
additional information
backbone NMR resonance assignments for the full length TrmD protein of Pseudomonas aeruginosa and secondary structure analysis
Pseudomonas aeruginosa
physiological function
methylation of guanine at position 37 in RNA is critical for bacterial growth, as this reaction is essential for preventing reading frame shifts during translation and therefore for maintaining the fidelity of protein synthesis. This methylation is catalyzed by the tRNA (guanine37-N1)-methyltransferase (TrmD). TrmD uses S-adenosyl-l-methionine (SAM) as a cofactor and transfers the methyl group to the N1 atom of G37 in tRNA. Bacterial tRNA (guanine37-N1)-methyltransferase (TrmD) plays important roles in translation. and is critical for growth of Pseudomonas aeruginosa
Pseudomonas aeruginosa
Other publictions for EC 2.1.1.228
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Protein Variants
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
kcat/KM [mM/s]
kcat/KM [mM/s] (protein specific)
755697
Zhong
Targeting the bacterial epitr ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
ACS Infect. Dis.
5
326-335
2019
-
1
1
-
-
-
63
2
-
-
-
2
-
8
-
-
-
-
-
-
-
-
6
-
3
-
-
1
2
-
-
-
1
-
-
63
-
1
1
1
-
-
-
63
63
-
2
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
1
2
-
-
-
-
-
1
1
-
2
2
756152
Li
Backbone resonance assignment ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
Biomol. NMR Assign.
13
327-332
2019
-
1
1
-
-
-
1
-
-
-
-
2
-
7
-
-
1
-
-
1
-
-
2
2
2
-
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
1
-
1
-
-
2
2
-
-
-
-
1
-
-
-
-
2
2
-
-
-
756153
Li
Backbone resonance assignment ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
Biomol. NMR Assign.
13
49-53
2019
-
-
1
-
-
-
2
-
-
-
-
2
-
6
-
-
1
-
-
-
-
-
2
-
2
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
2
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
756774
Hou
Codon-specific translation by ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720, Saccharomyces cerevisiae ATCC 204508
Front. Genet.
10
713
2019
-
-
-
-
1
-
-
-
-
2
-
7
-
8
-
-
-
-
-
-
-
-
7
-
4
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
1
8
8
1
-
-
757408
Whitehouse
Development of inhibitors aga ...
Mycobacteroides abscessus
J. Med. Chem.
62
7210-7232
2019
-
1
1
1
-
-
50
-
-
-
-
1
-
6
-
-
1
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
1
-
-
-
50
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
757409
Zhong
Thienopyrimidinone derivative ...
Staphylococcus aureus, Mycobacterium tuberculosis, Pseudomonas aeruginosa
J. Med. Chem.
62
7788-7805
2019
-
-
3
2
-
3
42
3
-
2
-
3
-
5
-
-
-
-
-
-
-
-
3
-
12
1
-
-
-
1
-
-
3
-
-
26
-
-
3
3
2
-
3
26
42
-
3
-
2
-
3
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
757863
Jin
AtTrm5a catalyses 1-methylgua ...
Arabidopsis thaliana
Nucleic Acids Res.
47
883-898
2019
-
-
1
-
1
-
-
-
1
1
-
2
-
8
-
-
1
-
-
2
-
-
2
-
3
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
2
-
-
-
1
-
2
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
758329
Jaroensuk
Crystal structure and catalyt ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
RNA
25
1481-1496
2019
-
1
1
1
1
-
1
3
-
2
-
2
-
10
-
-
1
-
-
-
-
-
14
2
5
1
-
-
2
1
-
-
1
3
-
-
-
1
1
1
1
1
-
-
1
3
3
-
2
-
2
-
-
-
1
-
-
-
-
14
2
1
-
-
2
1
-
-
-
-
2
2
-
2
2
757201
Zhou
A hypertension-associated mit ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii NBRC 100440, Methanocaldococcus jannaschii DSM 2661, Methanocaldococcus jannaschii ATCC 43067, Methanocaldococcus jannaschii JAL-1, Methanocaldococcus jannaschii JCM 10045
J. Biol. Chem.
293
1425-1438
2018
-
-
-
-
1
-
-
-
-
-
-
6
-
17
-
-
-
-
-
-
-
-
12
-
5
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
755960
Wu
The crystal structure of the ...
Pyrococcus abyssi
Biochem. Biophys. Res. Commun.
493
240-245
2017
-
-
1
1
1
-
-
-
-
-
-
1
-
9
-
-
1
-
-
-
-
-
2
1
4
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
4
4
-
-
-
756161
Goto-Ito
Trm5 and TrmD two enzymes fr ...
Escherichia coli, Haemophilus influenzae, Methanocaldococcus jannaschii, Pyrococcus abyssi, Pyrococcus abyssi Orsay, Methanocaldococcus jannaschii NBRC 100440, Haemophilus influenzae RD, Methanocaldococcus jannaschii DSM 2661, Methanocaldococcus jannaschii ATCC 43067, Methanocaldococcus jannaschii JAL-1, Haemophilus influenzae DSM 11121, Haemophilus influenzae KW20, Haemophilus influenzae ATCC 51907, Methanocaldococcus jannaschii JCM 10045
Biomolecules
7
32
2017
-
-
-
1
-
-
-
4
-
-
-
16
-
36
-
-
-
-
-
-
-
-
30
4
10
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
1
-
-
-
-
-
4
-
-
-
16
-
-
-
-
-
-
-
-
30
4
-
-
-
-
-
-
-
-
-
14
14
-
-
-
756163
Hori
Transfer RNA methyltransferas ...
Haemophilus influenzae, Aquifex aeolicus, Escherichia coli
Biomolecules
7
E23
2017
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
6
3
3
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
6
3
-
-
-
-
-
-
-
-
-
7
7
-
-
-
756631
Hou
TrmD A methyl transferase fo ...
Haemophilus influenzae, Aquifex aeolicus, Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
Enzymes
41
89-115
2017
-
4
4
-
-
-
-
-
-
16
-
12
-
8
-
-
-
-
-
-
-
-
12
8
6
-
-
-
-
-
-
-
4
-
-
-
-
4
4
4
-
-
-
-
-
-
-
-
16
-
12
-
-
-
-
-
-
-
-
12
8
-
-
-
-
-
-
-
-
-
16
16
-
-
-
758348
Wang
Structural insight into the m ...
Methanocaldococcus jannaschii, Pyrococcus abyssi, Methanocaldococcus jannaschii DSM 2661
Sci. Adv.
3
e1700195
2017
-
-
1
2
7
-
-
-
-
2
-
4
-
6
-
-
1
-
-
-
-
-
7
1
6
2
-
-
-
2
-
-
2
-
-
-
-
-
1
2
2
7
-
-
-
-
-
-
2
-
4
-
-
-
1
-
-
-
-
7
1
2
-
-
-
2
-
-
-
-
4
4
-
-
-
757790
Christian
Methyl transfer by substrate ...
Haemophilus influenzae
Nat. Struct. Mol. Biol.
23
941-948
2016
-
1
-
-
-
-
1
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
1
2
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
1
-
-
-
-
-
-
-
-
-
4
4
-
-
-
758324
Urbonavicius
Evolution of tRNAPhe imG2 met ...
Nanoarchaeum equitans, Pyrococcus abyssi, Pyrococcus abyssi Orsay
RNA
22
1871-1883
2016
-
-
2
-
7
-
-
-
-
2
-
11
-
4
-
-
2
-
-
-
-
-
19
-
14
2
-
-
-
2
-
-
2
-
-
-
-
-
2
2
-
7
-
-
-
-
-
-
2
-
11
-
-
-
2
-
-
-
-
19
-
2
-
-
-
2
-
-
-
-
8
8
-
-
-
758376
Wang
Crystal structures of the bif ...
Pyrococcus abyssi
Sci. Rep.
6
33553
2016
-
-
1
1
2
-
-
-
-
1
-
2
-
9
-
-
1
-
-
-
-
-
3
2
5
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
2
-
-
-
-
-
-
1
-
2
-
-
-
1
-
-
-
-
3
2
-
-
-
-
-
-
-
-
-
4
4
-
-
-
735421
Powell
TRMT5 mutations cause a defect ...
Homo sapiens
Am. J. Hum. Genet.
97
319-328
2015
-
-
1
-
3
-
-
-
1
-
-
1
-
7
-
-
-
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
3
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Transfer RNA methyltransferase ...
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737228
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The T. brucei TRM5 methyltrans ...
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Recognition of guanosine by di ...
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721029
Lahoud
Differentiating analogous tRNA ...
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RNA
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2011
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2
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721026
Christian
Mechanism of N-methylation by ...
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2010
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1
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18
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701123
Goto-Ito
Crystal structure of archaeal ...
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1
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1
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1
1
712103
Toyooka
Stabilization of tRNA (mG37) m ...
Aquifex aeolicus
Genes Cells
13
807-816
2008
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1
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687609
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Yeast mitochondrial initiator ...
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Distinct determinants of tRNA ...
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5
673850
Takeda
The substrate specificity of t ...
Aquifex aeolicus
Genes Cells
11
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2006
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711197
Christian
Catalysis by the second class ...
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Biochemistry
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2006
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1
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11
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1
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11
661154
Brule
Isolation and characterization ...
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662010
O'Dwyer
Characterization of Streptococ ...
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2
662622
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Distinct origins of tRNA(m1G37 ...
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2004
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661585
Ahn
Crystal structure of tRNA(m1G3 ...
Haemophilus influenzae
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2593-2603
2003
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712737
Elkins
Insights into catalysis by a k ...
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713482
Liu
Crystal structure of tRNA (m1G ...
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2003
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710708
Kim
Crystallization and preliminar ...
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