Literature summary for 2.1.1.228 extracted from

Zhong, W.; Koay, A.; Ngo, A.; Li, Y.; Nah, Q.; Wong, Y.H.; Chionh, Y.H.; Ng, H.Q.; Koh-Stenta, X.; Poulsen, A.; Foo, K.; McBee, M.; Choong, M.L.; El Sahili, A.; Kang, C.; Matter, A.; Lescar, J.; Hill, J.; Dedon, P.
Targeting the bacterial epitranscriptome for antibiotic development discovery of novel tRNA-(N1G37) methyltransferase (TrmD) inhibitors (2019), ACS Infect. Dis., 5, 326-335 .

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Application

Application	Comment	Organism
drug development	bacterial tRNA modification synthesis pathways are critical to cell survival under stress and thus represent ideal mechanism-based targets for antibiotic development. tRNA-(N1G37) methyltransferase (TrmD) is a target, which is conserved and essential in many bacterial pathogens	Pseudomonas aeruginosa

Cloned(Commentary)

Cloned (Comment)	Organism
gene trmD, sequence comparisons	Pseudomonas aeruginosa

Inhibitors

Inhibitors	Comment	Organism
(4-aminoquinazolin-2-yl)methyl 3-aminopyrazine-2-carboxylate	-	Pseudomonas aeruginosa
(4-oxo-3,4-dihydrothieno[3,2-d]pyrimidin-2-yl)methyl 2-(furan-2-yl)quinoline-4-carboxylate	-	Pseudomonas aeruginosa
1-(1H-pyrrol-2-yl)-2-[(thieno[2,3-d]pyrimidin-4-yl)oxy]ethan-1-one	-	Pseudomonas aeruginosa
1-(2-phenylpyrimidin-4-yl)-N-[(4-propoxyphenyl)methyl]piperidine-4-carboxamide	-	Pseudomonas aeruginosa
1-(3-chlorophenyl)-5-ethyl-N-(4H-1,2,4-triazol-3-yl)-1H-pyrazole-4-carboxamide	-	Pseudomonas aeruginosa
1-[(2-chlorophenyl)methyl]-N-(4H-1,2,4-triazol-3-yl)-1H-pyrazole-4-carboxamide	-	Pseudomonas aeruginosa
1-[7-(3,4-dimethylbenzoyl)-2H-[1,3]dioxolo[4,5-g]quinolin-8-yl]piperidine-4-carboxamide	-	Pseudomonas aeruginosa
2-(1H-inden-2-yl)-5-[2-(1H-indol-3-yl)ethyl]-4-methyl-1H-pyrazolo[4,3-c]pyridine-3,6(2H,5H)-dione	-	Pseudomonas aeruginosa
2-(5-chlorothiophen-2-yl)-2-oxoethyl (3,4-dimethoxyphenyl)acetate	-	Pseudomonas aeruginosa
2-(8-fluoro-3,4-dihydroquinolin-1(2H)-yl)-N-(quinolin-5-yl)acetamide	-	Pseudomonas aeruginosa
2-oxo-2-(2-oxo-2,3-dihydro-1H-benzimidazol-5-yl)ethyl 2-methylquinoline-4-carboxylate	-	Pseudomonas aeruginosa
2-oxo-2-(2-oxo-2,3-dihydro-1H-benzimidazol-5-yl)ethyl 3-(cyclopropylsulfamoyl)thiophene-2-carboxylate	-	Pseudomonas aeruginosa
2-oxo-2-(3-sulfamoylanilino)ethyl 2-(furan-2-yl)quinoline-4-carboxylate	-	Pseudomonas aeruginosa
2-oxo-2-[(quinolin-5-yl)amino]ethyl 1-(5-carbamoylpyridin-2-yl)piperidine-4-carboxylate	-	Pseudomonas aeruginosa
2-phenyl-7-(quinoline-6-carbonyl)-5,6,7,8-tetrahydropyrazolo[1,5-a]pyrido[4,3-d]pyrimidin-9(1H)-one	-	Pseudomonas aeruginosa
2-[(3,5-dichloropyridin-2-yl)amino]-2-oxoethyl 5,6-dihydro-4H-cyclopenta[b]thiophene-2-carboxylate	-	Pseudomonas aeruginosa
2-[(4-acetylbenzene-1-sulfonyl)amino]thiophene-3-carboxamide	-	Pseudomonas aeruginosa
2-[(5-chloropyridin-2-yl)amino]-2-oxoethyl 2-(furan-2-yl)quinoline-4-carboxylate	-	Pseudomonas aeruginosa
2-[2-(1-methyl-2-phenyl-2,3-dihydro-1H-indol-3-yl)-2-oxoethyl]-1,3-dioxo-1,2,3,5,6,7-hexahydropyrrolo[1,2-c]pyrimidine-4-carbonitrile	-	Pseudomonas aeruginosa
2-[2-(3-chlorophenoxy)acetamido]thiophene-3-carboxamide	-	Pseudomonas aeruginosa
2-[2-(4-oxocinnolin-1(4H)-yl)acetamido]-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carboxamide	-	Pseudomonas aeruginosa
2-[3-(benzenesulfonyl)-7-methyl-4-oxo-1,8-naphthyridin-1(4H)-yl]-N-methyl-N-phenylacetamide	-	Pseudomonas aeruginosa
2-[5-[2-(methylamino)-1,3-thiazol-4-yl]thiophen-2-yl]acetamide	-	Pseudomonas aeruginosa
3-(1H-pyrrol-1-yl)-N-[4-[(1,3-thiazol-2-yl)sulfamoyl]phenyl]benzamide	-	Pseudomonas aeruginosa
3-(4-methoxyphenyl)-7-[(1H-pyrrol-2-yl)methyl]-5,6,7,8-tetrahydro[1,2,4]triazolo[4,3-a]pyrazine	-	Pseudomonas aeruginosa
3-oxo-N-(5,6,7,8-tetrahydronaphthalen-1-yl)-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide	-	Pseudomonas aeruginosa
3-oxo-N-[1-[4-(1H-1,2,4-triazol-1-yl)phenyl]ethyl]-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide	-	Pseudomonas aeruginosa
3-oxo-N-[[2-(thiophen-2-yl)-1,3-oxazol-4-yl]methyl]-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide	-	Pseudomonas aeruginosa
4-[(tetrazolo[1,5-b]pyridazin-6-yl)amino]benzamide	-	Pseudomonas aeruginosa
4-[3-(8-hydroxyimidazo[1,2-a]pyridin-2-yl)-2,5-dimethyl-1H-pyrrol-1-yl]benzene-1-sulfonamide	-	Pseudomonas aeruginosa
4-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]thiomorpholine	-	Pseudomonas aeruginosa
5,7-dimethyl-N-(4H-1,2,4-triazol-3-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide	-	Pseudomonas aeruginosa
5-(5-methylthiophen-2-yl)thieno[2,3-d]pyrimidin-4(3H)-one	-	Pseudomonas aeruginosa
5-[2-(1,4-dioxa-8-azaspiro[4.5]decan-8-yl)-2-oxoethyl]-2-(3-fluorophenyl)-7-methyl-3-(1H-pyrrol-1-yl)-2,3,3a,5-tetrahydro-4H-pyrazolo[3,4-d]pyridazin-4-one	-	Pseudomonas aeruginosa
6-(2-[[(furan-2-yl)methyl]amino]-1,3-thiazol-4-yl)-3,4-dihydroquinolin-2(1H)-one	-	Pseudomonas aeruginosa
6-[5-(thiophen-2-yl)-1,2,4-oxadiazol-3-yl][1,2,4]triazolo[4,3-a]pyridine	-	Pseudomonas aeruginosa
ethyl 4-[(3-cyano-6,7-dimethoxyquinolin-4-yl)amino]benzoate	-	Pseudomonas aeruginosa
additional information	high-throughput small-molecule library inhibitor screening, antibacterial growth inhibitory activities and haemolytic activity of selected TrmD inhibitors, binding affinity confirmed by thermal stability and surface plasmon resonance, overview	Pseudomonas aeruginosa
N-(2-methoxy-5-methylphenyl)-N'-[1,2,4]triazolo[4,3-a]pyridin-3-ylurea	-	Pseudomonas aeruginosa
N-(3,4-dimethylphenyl)-5,6-dihydro-4H-cyclopenta[d][1,2]oxazole-3-carboxamide	-	Pseudomonas aeruginosa
N-(3-acetylphenyl)-N2-[5-chloro-2-(pyrrolidin-1-yl)phenyl]glycinamide	-	Pseudomonas aeruginosa
N-(3-carbamoyl-5,6-dihydro-4H-cyclopenta[b]thiophen-2-yl)-4-chloropyridine-2-carboxamide	-	Pseudomonas aeruginosa
N-(3-carbamoylthiophen-2-yl)-2-(pyridin-4-yl)quinoline-4-carboxamide	-	Pseudomonas aeruginosa
N-(3-cyanothiophen-2-yl)-2-(furan-2-yl)quinoline-4-carboxamide	-	Pseudomonas aeruginosa
N-([1-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]piperidin-4-yl]methyl)-1H-indole-2-carboxamide	-	Pseudomonas aeruginosa
N-([2-[(morpholin-4-yl)methyl]phenyl]methyl)-2-(thiophene-2-carbonyl)benzamide	-	Pseudomonas aeruginosa
N-methyl-1-[2-([[2-(pyridin-3-yl)quinazolin-4-yl]amino]methyl)phenyl]methanesulfonamide	-	Pseudomonas aeruginosa
N-methyl-N-[(oxan-4-yl)methyl]-2-(thiophen-3-yl)acetamide	-	Pseudomonas aeruginosa
N-methyl-N-[[2-(pyrrolidin-1-yl)phenyl]methyl]-3-(1H-pyrrol-1-yl)benzamide	-	Pseudomonas aeruginosa
N-[(3,5-dimethyl-1,2-oxazol-4-yl)methyl]quinoline-6-carboxamide	-	Pseudomonas aeruginosa
N-[1-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]piperidin-3-yl]-1H-indole-2-carboxamide	-	Pseudomonas aeruginosa
N-[1-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]piperidin-3-yl]-2-(thiophen-2-yl)acetamide	-	Pseudomonas aeruginosa
N-[2-oxo-2-[(1,3,5-trimethyl-4,5-dihydro-1H-pyrazol-4-yl)amino]ethyl]-5,6-dihydro-4H-cyclopenta[b]thiophene-2-carboxamide	-	Pseudomonas aeruginosa
N-[3-(pyridin-4-yl)-1,2-oxazol-5-yl]-5,6,7,8-tetrahydronaphthalene-2-sulfonamide	-	Pseudomonas aeruginosa
N-[3-([1,3]thiazolo[5,4-b]pyridin-2-yl)phenyl]quinoline-2-carboxamide	-	Pseudomonas aeruginosa
N-[3-[(3-fluoro-4-methylphenyl)carbamoyl]phenyl]-1-([1,3]thiazolo[5,4-b]pyridin-2-yl)piperidine-3-carboxamide	-	Pseudomonas aeruginosa
N-[4-(2-amino-2-oxoethyl)-1,3-thiazol-2-yl]-3-(3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl)propanamide	-	Pseudomonas aeruginosa
N-[4-chloro-3-(pyrrolidine-1-sulfonyl)phenyl]-3-oxo-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide	-	Pseudomonas aeruginosa
N-[[4-(piperidine-1-carbonyl)phenyl]methyl]-2-(thiophene-2-carbonyl)benzamide	-	Pseudomonas aeruginosa
sinefungin	-	Pseudomonas aeruginosa
suramin	-	Pseudomonas aeruginosa
[4-(hydroxymethyl)-2,8-diazaspiro[4.5]decan-2-yl][1-(4-methoxyphenyl)cyclohexyl]methanone	-	Pseudomonas aeruginosa
[5-(furan-2-yl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-2-yl]methyl pyrazine-2-carboxylate	-	Pseudomonas aeruginosa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0012	-	guanine37 in tRNALeu(CAG)	pH and temperature not specified in the publication	Pseudomonas aeruginosa
0.0018	-	S-adenosyl-L-methionine	pH and temperature not specified in the publication	Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + guanine37 in tRNA	Pseudomonas aeruginosa	-	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?
S-adenosyl-L-methionine + guanine37 in tRNA	Pseudomonas aeruginosa UCBPP-PA14	-	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Pseudomonas aeruginosa	Q02RL6	-	-
Pseudomonas aeruginosa UCBPP-PA14	Q02RL6	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
additional information	TrmD luminescence assay development	Pseudomonas aeruginosa	?	-	-
additional information	TrmD luminescence assay development	Pseudomonas aeruginosa UCBPP-PA14	?	-	-
S-adenosyl-L-methionine + guanine37 in tRNA	-	Pseudomonas aeruginosa	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?
S-adenosyl-L-methionine + guanine37 in tRNA	-	Pseudomonas aeruginosa UCBPP-PA14	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA	-	?
S-adenosyl-L-methionine + guanine37 in tRNALeu(CAG)	-	Pseudomonas aeruginosa	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu(CAG)	-	?
S-adenosyl-L-methionine + guanine37 in tRNALeu(CAG)	-	Pseudomonas aeruginosa UCBPP-PA14	S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu(CAG)	-	?

Synonyms

Synonyms	Comment	Organism
PaTrmD	-	Pseudomonas aeruginosa
TrmD	-	Pseudomonas aeruginosa
tRNA-(N1G37) methyltransferase	-	Pseudomonas aeruginosa

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
additional information	-	thermostabilization of TrmD by compounds belonging to the pyridine-pyrazol-piperidin chemical cluster, overview	Pseudomonas aeruginosa

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.0105	-	S-adenosyl-L-methionine	pH and temperature not specified in the publication	Pseudomonas aeruginosa
0.0113	-	guanine37 in tRNALeu(CAG)	pH and temperature not specified in the publication	Pseudomonas aeruginosa

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Pseudomonas aeruginosa

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor
0.00011	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	1-[(2-chlorophenyl)methyl]-N-(4H-1,2,4-triazol-3-yl)-1H-pyrazole-4-carboxamide
0.00031	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	3-(1H-pyrrol-1-yl)-N-[4-[(1,3-thiazol-2-yl)sulfamoyl]phenyl]benzamide
0.00049	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	5-(5-methylthiophen-2-yl)thieno[2,3-d]pyrimidin-4(3H)-one
0.00053	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[(5-chloropyridin-2-yl)amino]-2-oxoethyl 2-(furan-2-yl)quinoline-4-carboxylate
0.0007	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[(3,5-dichloropyridin-2-yl)amino]-2-oxoethyl 5,6-dihydro-4H-cyclopenta[b]thiophene-2-carboxylate
0.00093	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-phenyl-7-(quinoline-6-carbonyl)-5,6,7,8-tetrahydropyrazolo[1,5-a]pyrido[4,3-d]pyrimidin-9(1H)-one
0.001	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[[4-(piperidine-1-carbonyl)phenyl]methyl]-2-(thiophene-2-carbonyl)benzamide
0.0011	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-(5-chlorothiophen-2-yl)-2-oxoethyl (3,4-dimethoxyphenyl)acetate
0.0013	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-([1-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]piperidin-4-yl]methyl)-1H-indole-2-carboxamide
0.0013	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-methyl-N-[[2-(pyrrolidin-1-yl)phenyl]methyl]-3-(1H-pyrrol-1-yl)benzamide
0.0014	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[2-(1-methyl-2-phenyl-2,3-dihydro-1H-indol-3-yl)-2-oxoethyl]-1,3-dioxo-1,2,3,5,6,7-hexahydropyrrolo[1,2-c]pyrimidine-4-carbonitrile
0.0015	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-oxo-2-(3-sulfamoylanilino)ethyl 2-(furan-2-yl)quinoline-4-carboxylate
0.0016	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-(3-carbamoyl-5,6-dihydro-4H-cyclopenta[b]thiophen-2-yl)-4-chloropyridine-2-carboxamide
0.0017	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	(4-oxo-3,4-dihydrothieno[3,2-d]pyrimidin-2-yl)methyl 2-(furan-2-yl)quinoline-4-carboxylate
0.0017	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	1-(3-chlorophenyl)-5-ethyl-N-(4H-1,2,4-triazol-3-yl)-1H-pyrazole-4-carboxamide
0.0017	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	3-(4-methoxyphenyl)-7-[(1H-pyrrol-2-yl)methyl]-5,6,7,8-tetrahydro[1,2,4]triazolo[4,3-a]pyrazine
0.0019	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[2-(3-chlorophenoxy)acetamido]thiophene-3-carboxamide
0.0019	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	4-[(tetrazolo[1,5-b]pyridazin-6-yl)amino]benzamide
0.0019	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[4-(2-amino-2-oxoethyl)-1,3-thiazol-2-yl]-3-(3,5-dimethyl-1-phenyl-1H-pyrazol-4-yl)propanamide
0.002	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[5-[2-(methylamino)-1,3-thiazol-4-yl]thiophen-2-yl]acetamide
0.0021	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-(3-cyanothiophen-2-yl)-2-(furan-2-yl)quinoline-4-carboxamide
0.0021	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[3-([1,3]thiazolo[5,4-b]pyridin-2-yl)phenyl]quinoline-2-carboxamide
0.0025	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[2-(4-oxocinnolin-1(4H)-yl)acetamido]-5,6-dihydro-4H-cyclopenta[b]thiophene-3-carboxamide
0.0025	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	5,7-dimethyl-N-(4H-1,2,4-triazol-3-yl)pyrazolo[1,5-a]pyrimidine-3-carboxamide
0.0027	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[3-(benzenesulfonyl)-7-methyl-4-oxo-1,8-naphthyridin-1(4H)-yl]-N-methyl-N-phenylacetamide
0.0028	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-(2-methoxy-5-methylphenyl)-N'-[1,2,4]triazolo[4,3-a]pyridin-3-ylurea
0.0029	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-[(4-acetylbenzene-1-sulfonyl)amino]thiophene-3-carboxamide
0.0029	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	4-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]thiomorpholine
0.0029	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[1-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]piperidin-3-yl]-1H-indole-2-carboxamide
0.0031	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[4-chloro-3-(pyrrolidine-1-sulfonyl)phenyl]-3-oxo-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide
0.0034	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-oxo-2-(3-sulfamoylanilino)ethyl 2-(furan-2-yl)quinoline-4-carboxylate
0.0034	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-oxo-2-[(quinolin-5-yl)amino]ethyl 1-(5-carbamoylpyridin-2-yl)piperidine-4-carboxylate
0.0036	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	5-[2-(1,4-dioxa-8-azaspiro[4.5]decan-8-yl)-2-oxoethyl]-2-(3-fluorophenyl)-7-methyl-3-(1H-pyrrol-1-yl)-2,3,3a,5-tetrahydro-4H-pyrazolo[3,4-d]pyridazin-4-one
0.0038	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-(1H-inden-2-yl)-5-[2-(1H-indol-3-yl)ethyl]-4-methyl-1H-pyrazolo[4,3-c]pyridine-3,6(2H,5H)-dione
0.0039	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[(3,5-dimethyl-1,2-oxazol-4-yl)methyl]quinoline-6-carboxamide
0.0043	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-methyl-1-[2-([[2-(pyridin-3-yl)quinazolin-4-yl]amino]methyl)phenyl]methanesulfonamide
0.0045	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-oxo-2-(2-oxo-2,3-dihydro-1H-benzimidazol-5-yl)ethyl 2-methylquinoline-4-carboxylate
0.0047	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-(3-acetylphenyl)-N2-[5-chloro-2-(pyrrolidin-1-yl)phenyl]glycinamide
0.0049	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	1-(1H-pyrrol-2-yl)-2-[(thieno[2,3-d]pyrimidin-4-yl)oxy]ethan-1-one
0.005	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-(3,4-dimethylphenyl)-5,6-dihydro-4H-cyclopenta[d][1,2]oxazole-3-carboxamide
0.0053	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-oxo-2-(2-oxo-2,3-dihydro-1H-benzimidazol-5-yl)ethyl 3-(cyclopropylsulfamoyl)thiophene-2-carboxylate
0.0053	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	[5-(furan-2-yl)-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-2-yl]methyl pyrazine-2-carboxylate
0.0054	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	6-[5-(thiophen-2-yl)-1,2,4-oxadiazol-3-yl][1,2,4]triazolo[4,3-a]pyridine
0.0056	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	4-[3-(8-hydroxyimidazo[1,2-a]pyridin-2-yl)-2,5-dimethyl-1H-pyrrol-1-yl]benzene-1-sulfonamide
0.0058	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	1-[7-(3,4-dimethylbenzoyl)-2H-[1,3]dioxolo[4,5-g]quinolin-8-yl]piperidine-4-carboxamide
0.0059	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	[4-(hydroxymethyl)-2,8-diazaspiro[4.5]decan-2-yl][1-(4-methoxyphenyl)cyclohexyl]methanone
0.006	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	ethyl 4-[(3-cyano-6,7-dimethoxyquinolin-4-yl)amino]benzoate
0.006	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-(3-carbamoylthiophen-2-yl)-2-(pyridin-4-yl)quinoline-4-carboxamide
0.0062	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-methyl-N-[(oxan-4-yl)methyl]-2-(thiophen-3-yl)acetamide
0.0066	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	2-(8-fluoro-3,4-dihydroquinolin-1(2H)-yl)-N-(quinolin-5-yl)acetamide
0.0068	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[2-oxo-2-[(1,3,5-trimethyl-4,5-dihydro-1H-pyrazol-4-yl)amino]ethyl]-5,6-dihydro-4H-cyclopenta[b]thiophene-2-carboxamide
0.0083	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	sinefungin
0.0087	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	3-oxo-N-(5,6,7,8-tetrahydronaphthalen-1-yl)-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide
0.0087	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[3-(pyridin-4-yl)-1,2-oxazol-5-yl]-5,6,7,8-tetrahydronaphthalene-2-sulfonamide
0.0091	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	suramin
0.0091	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	3-oxo-N-[1-[4-(1H-1,2,4-triazol-1-yl)phenyl]ethyl]-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide
0.0092	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	(4-aminoquinazolin-2-yl)methyl 3-aminopyrazine-2-carboxylate
0.0097	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	3-oxo-N-[[2-(thiophen-2-yl)-1,3-oxazol-4-yl]methyl]-3,4-dihydro-2H-1,4-benzoxazine-6-carboxamide
0.01	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-([2-[(morpholin-4-yl)methyl]phenyl]methyl)-2-(thiophene-2-carbonyl)benzamide
0.011	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	6-(2-[[(furan-2-yl)methyl]amino]-1,3-thiazol-4-yl)-3,4-dihydroquinolin-2(1H)-one
0.012	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[1-[3-(pyridin-4-yl)-1H-pyrazol-5-yl]piperidin-3-yl]-2-(thiophen-2-yl)acetamide
0.013	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	1-(2-phenylpyrimidin-4-yl)-N-[(4-propoxyphenyl)methyl]piperidine-4-carboxamide
0.014	-	pH and temperature not specified in the publication	Pseudomonas aeruginosa	N-[3-[(3-fluoro-4-methylphenyl)carbamoyl]phenyl]-1-([1,3]thiazolo[5,4-b]pyridin-2-yl)piperidine-3-carboxamide

General Information

General Information	Comment	Organism
additional information	active site structure in complex with S-adenosyl-L-methionine, overview	Pseudomonas aeruginosa

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
5.83	-	S-adenosyl-L-methionine	pH and temperature not specified in the publication	Pseudomonas aeruginosa
9.44	-	guanine37 in tRNALeu(CAG)	pH and temperature not specified in the publication	Pseudomonas aeruginosa