BRENDA - Enzyme Database
show all sequences of 2.1.1.228

Recognition of guanosine by dissimilar tRNA methyltransferases

Sakaguchi, R.; Giessing, A.; Dai, Q.; Lahoud, G.; Liutkeviciute, Z.; Klimasauskas, S.; Piccirilli, J.; Kirpekar, F.; Hou, Y.M.; RNA 18, 1687-1701 (2012) View publication on PubMedView publication on EuropePMC

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
single turnover kinetics and thermodynamic analysis of effect of different guanosine analogues on m1G37-tRNA synthesis, kinetic analysis, overview
Escherichia coli
additional information
-
additional information
single turnover kinetics and thermodynamic analysis of effect of different guanosine analogues on m1G37-tRNA synthesis, kinetic analysis, overview
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + guanine37 in tRNA
Escherichia coli
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Methanocaldococcus jannaschii
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Escherichia coli
-
-
-
Methanocaldococcus jannaschii
-
-
-
Reaction
Reaction
Commentary
Organism
Reaction ID
S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
substrate recognition and reaction mechanisms, overview
Escherichia coli
S-adenosyl-L-methionine + guanine37 in tRNA = S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
substrate recognition and reaction mechanisms, overview
Methanocaldococcus jannaschii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
additional information
recognition of N1 of G37 in tRNA is essential for translational fidelity in all biological domains, Trm5 shows a less rigid requirement of guanosine functional groups. Replacment of functional groups of G37 by guanosine analogues, i.e. deoxyG, 6-thioG, inosine, and 2-aminopurine, in MjtRNACys, to design the optimal substrate for Trm5
721035
Methanocaldococcus jannaschii
?
-
-
-
?
additional information
recognition of N1 of G37 in tRNA is essential for translational fidelity in all biological domains, TrmD shows a more rigid requirement of guanosine functional groups. Replacment of functional groups of G37 by guanosine analogues, i.e. deoxyG, 6-thioG, inosine, and 2-aminopurine, in EctRNALeu, to design the optimal substrate for TrmD. All but deoxyG of these analogs probed the Watson-Crick basepairing interface of G37
721035
Escherichia coli
?
-
-
-
?
S-adenosyl-L-methionine + guanine36 in tRNALeu
G36-substituted tRNA substrate Escherichia coli tRNALeu, Trm5 shows a lack of discrimination between the two sequences of G36 and G37
721035
Methanocaldococcus jannaschii
S-adenosyl-L-homocysteine + N1-methylguanine36 in tRNALeu
-
-
-
?
S-adenosyl-L-methionine + guanine36 in tRNALeu
G36-substituted tRNA substrate Escherichia coli tRNALeu, TrmD shows a 90fold reduced catalytic efficiency, discrimination between the two sequences of G36 and G37
721035
Escherichia coli
S-adenosyl-L-homocysteine + N1-methylguanine36 in tRNALeu
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
721035
Escherichia coli
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
721035
Methanocaldococcus jannaschii
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Trm5 recognizes N1 and O6 of G37, but the exocyclic 2-amino group of G37 is dispensable for Trm5. Trm5 does not require G36
721035
Methanocaldococcus jannaschii
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
TrmD recognizes N1 and O6 of G37 and the exocyclic 2-amino group of G37 is important for TrmD, also TrmD requires G36 for synthesis of m1G37
721035
Escherichia coli
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNACys
Methanococcus jannaschii tRNACys
721035
Methanocaldococcus jannaschii
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACys
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNALeu
Escherichia coli tRNALeu
721035
Escherichia coli
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
TRM5
-
Methanocaldococcus jannaschii
TrmD
-
Escherichia coli
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Escherichia coli
S-adenosyl-L-methionine
-
Methanocaldococcus jannaschii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Escherichia coli
S-adenosyl-L-methionine
-
Methanocaldococcus jannaschii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
additional information
-
additional information
single turnover kinetics and thermodynamic analysis of effect of different guanosine analogues on m1G37-tRNA synthesis, kinetic analysis, overview
Escherichia coli
additional information
-
additional information
single turnover kinetics and thermodynamic analysis of effect of different guanosine analogues on m1G37-tRNA synthesis, kinetic analysis, overview
Methanocaldococcus jannaschii
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
S-adenosyl-L-methionine + guanine37 in tRNA
Escherichia coli
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Methanocaldococcus jannaschii
-
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
additional information
recognition of N1 of G37 in tRNA is essential for translational fidelity in all biological domains, Trm5 shows a less rigid requirement of guanosine functional groups. Replacment of functional groups of G37 by guanosine analogues, i.e. deoxyG, 6-thioG, inosine, and 2-aminopurine, in MjtRNACys, to design the optimal substrate for Trm5
721035
Methanocaldococcus jannaschii
?
-
-
-
?
additional information
recognition of N1 of G37 in tRNA is essential for translational fidelity in all biological domains, TrmD shows a more rigid requirement of guanosine functional groups. Replacment of functional groups of G37 by guanosine analogues, i.e. deoxyG, 6-thioG, inosine, and 2-aminopurine, in EctRNALeu, to design the optimal substrate for TrmD. All but deoxyG of these analogs probed the Watson-Crick basepairing interface of G37
721035
Escherichia coli
?
-
-
-
?
S-adenosyl-L-methionine + guanine36 in tRNALeu
G36-substituted tRNA substrate Escherichia coli tRNALeu, Trm5 shows a lack of discrimination between the two sequences of G36 and G37
721035
Methanocaldococcus jannaschii
S-adenosyl-L-homocysteine + N1-methylguanine36 in tRNALeu
-
-
-
?
S-adenosyl-L-methionine + guanine36 in tRNALeu
G36-substituted tRNA substrate Escherichia coli tRNALeu, TrmD shows a 90fold reduced catalytic efficiency, discrimination between the two sequences of G36 and G37
721035
Escherichia coli
S-adenosyl-L-homocysteine + N1-methylguanine36 in tRNALeu
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
721035
Escherichia coli
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
-
721035
Methanocaldococcus jannaschii
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
Trm5 recognizes N1 and O6 of G37, but the exocyclic 2-amino group of G37 is dispensable for Trm5. Trm5 does not require G36
721035
Methanocaldococcus jannaschii
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNA
TrmD recognizes N1 and O6 of G37 and the exocyclic 2-amino group of G37 is important for TrmD, also TrmD requires G36 for synthesis of m1G37
721035
Escherichia coli
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNACys
Methanococcus jannaschii tRNACys
721035
Methanocaldococcus jannaschii
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNACys
-
-
-
?
S-adenosyl-L-methionine + guanine37 in tRNALeu
Escherichia coli tRNALeu
721035
Escherichia coli
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu
-
-
-
?
General Information
General Information
Commentary
Organism
additional information
S-adenosyl-methionine-dependent m1G37-tRNA methyltransferases rapidly screen tRNA by direct recognition of G37 in order to monitor the global state of m1G37-tRNA
Escherichia coli
additional information
S-adenosyl-methionine-dependent m1G37-tRNA methyltransferases rapidly screen tRNA by direct recognition of G37 in order to monitor the global state of m1G37-tRNA
Methanocaldococcus jannaschii
General Information (protein specific)
General Information
Commentary
Organism
additional information
S-adenosyl-methionine-dependent m1G37-tRNA methyltransferases rapidly screen tRNA by direct recognition of G37 in order to monitor the global state of m1G37-tRNA
Escherichia coli
additional information
S-adenosyl-methionine-dependent m1G37-tRNA methyltransferases rapidly screen tRNA by direct recognition of G37 in order to monitor the global state of m1G37-tRNA
Methanocaldococcus jannaschii
Other publictions for EC 2.1.1.228
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
755697
Zhong
Targeting the bacterial epitr ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
ACS Infect. Dis.
5
326-335
2019
-
1
1
-
-
-
63
2
-
-
-
2
-
8
-
-
-
-
-
-
-
-
6
-
3
-
-
1
2
-
-
-
1
-
-
63
-
1
1
1
-
-
-
63
63
-
2
-
-
-
2
-
-
-
-
-
-
-
-
6
-
-
-
1
2
-
-
-
-
-
1
1
-
2
2
756152
Li
Backbone resonance assignment ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
Biomol. NMR Assign.
13
327-332
2019
-
1
1
-
-
-
1
-
-
-
-
2
-
7
-
-
1
-
-
1
-
-
2
2
2
-
-
-
-
1
-
-
1
-
-
-
-
1
1
1
-
-
-
-
1
-
-
-
-
-
2
-
-
-
1
-
1
-
-
2
2
-
-
-
-
1
-
-
-
-
2
2
-
-
-
756153
Li
Backbone resonance assignment ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
Biomol. NMR Assign.
13
49-53
2019
-
-
1
-
-
-
2
-
-
-
-
2
-
6
-
-
1
-
-
-
-
-
2
-
2
-
-
1
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
2
-
-
-
-
-
2
-
-
-
1
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
1
1
-
-
-
756774
Hou
Codon-specific translation by ...
Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720, Saccharomyces cerevisiae ATCC 204508
Front. Genet.
10
713
2019
-
-
-
-
1
-
-
-
-
2
-
7
-
8
-
-
-
-
-
-
-
-
7
-
4
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
1
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
7
-
-
-
-
-
-
-
-
-
1
8
8
1
-
-
757408
Whitehouse
Development of inhibitors aga ...
Mycobacteroides abscessus
J. Med. Chem.
62
7210-7232
2019
-
1
1
1
-
-
50
-
-
-
-
1
-
6
-
-
1
-
-
-
-
-
1
1
2
-
-
-
-
-
-
-
1
-
-
-
-
1
1
1
1
-
-
-
50
-
-
-
-
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
757409
Zhong
Thienopyrimidinone derivative ...
Staphylococcus aureus, Mycobacterium tuberculosis, Pseudomonas aeruginosa
J. Med. Chem.
62
7788-7805
2019
-
-
3
2
-
3
42
3
-
2
-
3
-
5
-
-
-
-
-
-
-
-
3
-
12
1
-
-
-
1
-
-
3
-
-
26
-
-
3
3
2
-
3
26
42
-
3
-
2
-
3
-
-
-
-
-
-
-
-
3
-
1
-
-
-
1
-
-
-
-
3
3
-
-
-
757863
Jin
AtTrm5a catalyses 1-methylgua ...
Arabidopsis thaliana
Nucleic Acids Res.
47
883-898
2019
-
-
1
-
1
-
-
-
1
1
-
2
-
8
-
-
1
-
-
2
-
-
2
-
3
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
-
1
-
-
-
-
-
1
1
-
2
-
-
-
1
-
2
-
-
2
-
1
-
-
-
1
-
-
-
-
2
2
-
-
-
758329
Jaroensuk
Crystal structure and catalyt ...
Pseudomonas aeruginosa, Pseudomonas aeruginosa UCBPP-PA14
RNA
25
1481-1496
2019
-
1
1
1
1
-
1
3
-
2
-
2
-
10
-
-
1
-
-
-
-
-
14
2
5
1
-
-
2
1
-
-
1
3
-
-
-
1
1
1
1
1
-
-
1
3
3
-
2
-
2
-
-
-
1
-
-
-
-
14
2
1
-
-
2
1
-
-
-
-
2
2
-
2
2
757201
Zhou
A hypertension-associated mit ...
Methanocaldococcus jannaschii, Methanocaldococcus jannaschii NBRC 100440, Methanocaldococcus jannaschii DSM 2661, Methanocaldococcus jannaschii ATCC 43067, Methanocaldococcus jannaschii JAL-1, Methanocaldococcus jannaschii JCM 10045
J. Biol. Chem.
293
1425-1438
2018
-
-
-
-
1
-
-
-
-
-
-
6
-
17
-
-
-
-
-
-
-
-
12
-
5
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
6
-
-
-
-
-
-
-
-
12
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
755960
Wu
The crystal structure of the ...
Pyrococcus abyssi
Biochem. Biophys. Res. Commun.
493
240-245
2017
-
-
1
1
1
-
-
-
-
-
-
1
-
9
-
-
1
-
-
-
-
-
2
1
4
1
-
-
-
1
-
-
1
-
-
-
-
-
1
1
1
1
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
1
1
-
-
-
1
-
-
-
-
4
4
-
-
-
756161
Goto-Ito
Trm5 and TrmD two enzymes fr ...
Escherichia coli, Haemophilus influenzae, Methanocaldococcus jannaschii, Pyrococcus abyssi, Pyrococcus abyssi Orsay, Methanocaldococcus jannaschii NBRC 100440, Haemophilus influenzae RD, Methanocaldococcus jannaschii DSM 2661, Methanocaldococcus jannaschii ATCC 43067, Methanocaldococcus jannaschii JAL-1, Haemophilus influenzae DSM 11121, Haemophilus influenzae KW20, Haemophilus influenzae ATCC 51907, Methanocaldococcus jannaschii JCM 10045
Biomolecules
7
32
2017
-
-
-
1
-
-
-
4
-
-
-
16
-
36
-
-
-
-
-
-
-
-
30
4
10
-
-
-
-
-
-
-
4
-
-
-
-
-
-
4
1
-
-
-
-
-
4
-
-
-
16
-
-
-
-
-
-
-
-
30
4
-
-
-
-
-
-
-
-
-
14
14
-
-
-
756163
Hori
Transfer RNA methyltransferas ...
Haemophilus influenzae, Aquifex aeolicus, Escherichia coli
Biomolecules
7
E23
2017
-
-
-
-
-
-
-
-
-
-
-
3
-
3
-
-
-
-
-
-
-
-
6
3
3
-
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
6
3
-
-
-
-
-
-
-
-
-
7
7
-
-
-
756631
Hou
TrmD A methyl transferase fo ...
Haemophilus influenzae, Aquifex aeolicus, Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium, Salmonella enterica subsp. enterica serovar Typhimurium SGSC1412, Salmonella enterica subsp. enterica serovar Typhimurium ATCC 700720
Enzymes
41
89-115
2017
-
4
4
-
-
-
-
-
-
16
-
12
-
8
-
-
-
-
-
-
-
-
12
8
6
-
-
-
-
-
-
-
4
-
-
-
-
4
4
4
-
-
-
-
-
-
-
-
16
-
12
-
-
-
-
-
-
-
-
12
8
-
-
-
-
-
-
-
-
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The T. brucei TRM5 methyltrans ...
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Lahoud
Differentiating analogous tRNA ...
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RNA
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2011
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Christian
Mechanism of N-methylation by ...
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2484-2492
2010
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701123
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Crystal structure of archaeal ...
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1
712103
Toyooka
Stabilization of tRNA (mG37) m ...
Aquifex aeolicus
Genes Cells
13
807-816
2008
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Christian
Catalysis by the second class ...
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661154
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662010
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Characterization of Streptococ ...
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Distinct origins of tRNA(m1G37 ...
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Ahn
Crystal structure of tRNA(m1G3 ...
Haemophilus influenzae
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2593-2603
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Elkins
Insights into catalysis by a k ...
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713482
Liu
Crystal structure of tRNA (m1G ...
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Crystallization and preliminar ...
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