KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | pre-steady-state and steady-state kinetics, rapid burst phase followed by a slower and linear phase in reaction, single-turnover and from steady-state analysis, overview | Methanocaldococcus jannaschii | |
additional information | - |
additional information | pre-steady-state and steady-state kinetics, time-dependent linear reaction, overview. TrmD exhibits half-of-the-sites reactivity in which only one of the two active sites is functional at a given time | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Methanocaldococcus jannaschii | Trm5 catalyzes methyl transfer to synthesize the m1G37 base at the 3' position adjacent to the tRNA anticodon | ? | - |
? | |
additional information | Escherichia coli | TrmD catalyzes methyl transfer to synthesize the m1G37 base at the 3' position adjacent to the tRNA anticodon | ? | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | Methanocaldococcus jannaschii | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Methanocaldococcus jannaschii | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Trm5 catalyzes methyl transfer to synthesize the m1G37 base at the 3' position adjacent to the tRNA anticodon | Methanocaldococcus jannaschii | ? | - |
? | |
additional information | TrmD catalyzes methyl transfer to synthesize the m1G37 base at the 3' position adjacent to the tRNA anticodon | Escherichia coli | ? | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Methanocaldococcus jannaschii | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | tight binding of Trm5 to products | ? | |
S-adenosyl-L-methionine + guanine37 in tRNA | substrate binding stoichiometry to TrmD, dissociation constants, overview | Escherichia coli | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | TrmD features a trefoil-knot active-site structure | Escherichia coli |
More | Trm5 features the Rossmann fold | Methanocaldococcus jannaschii |
Synonyms | Comment | Organism |
---|---|---|
TRM5 | - |
Methanocaldococcus jannaschii |
TrmD | - |
Escherichia coli |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | 0.12 is kchem in the first rapid burst turnover | Methanocaldococcus jannaschii | |
0.02 | - |
S-adenosyl-L-methionine | kcat in steady-state phase turnover, pH and temperature not specified in the publication | Methanocaldococcus jannaschii | |
0.09 | - |
S-adenosyl-L-methionine | pH and temperature not specified in the publication | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli | |
S-adenosyl-L-methionine | - |
Methanocaldococcus jannaschii |