Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
crystal structure of TrmD complexed with S-adenosyl homocysteine, determined at 2.5 A resolution | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
A202S | Km/Vmax for tRNA is 2fold higher than wild-type value | Escherichia coli |
A25S | Km/Vmax for tRNA is 2.9fold higher than wild-type value | Escherichia coli |
A70S | Km/Vmax for tRNA is 4fold higher than wild-type value | Escherichia coli |
C112A | Km/Vmax for tRNA is 7.6fold higher than wild-type value | Escherichia coli |
D119A | inactive mutant enzyme | Escherichia coli |
D128A | inactive mutant enzyme | Escherichia coli |
D135A | inactive mutant enzyme | Escherichia coli |
D169A | inactive mutant enzyme | Escherichia coli |
D169E | Km/Vmax for tRNA is 1.4fold higher than wild-type value | Escherichia coli |
D50A | Km/Vmax for tRNA is 4fold higher than wild-type value | Escherichia coli |
E116A | Km/Vmax for tRNA is 2fold higher than wild-type value | Escherichia coli |
E130A | Km/Vmax for tRNA is 2fold higher than wild-type value | Escherichia coli |
E142A | Km/Vmax for tRNA is 3.1fold higher than wild-type value | Escherichia coli |
G113A | Km/Vmax for tRNA is 5.3fold higher than wild-type value | Escherichia coli |
G117A | inactive mutant enzyme | Escherichia coli |
G134A | Km/Vmax for tRNA is 6.8fold higher than wild-type value | Escherichia coli |
G140A | Km/Vmax for tRNA is 8.5fold higher than wild-type value | Escherichia coli |
G141A | Km/Vmax for tRNA is 1.5fold lower than wild-type value | Escherichia coli |
G189A | Km/Vmax for tRNA is 8fold higher than wild-type value | Escherichia coli |
G55A | Km/Vmax for tRNA is 4.8fold higher than wild-type value | Escherichia coli |
G59A | inactive mutant enzyme | Escherichia coli |
G91A | inactive mutant enzyme | Escherichia coli |
H180A | Km/Vmax for tRNA is 5 fold higher than wild-type value | Escherichia coli |
I204A | inactive mutant enzyme | Escherichia coli |
L138A | Km/Vmax for tRNA is 1,7fold higher than wild-type value | Escherichia coli |
L196A | inactive mutant enzyme | Escherichia coli |
L197A | inactive mutant enzyme | Escherichia coli |
M60A | Km/Vmax for tRNA is 2.7fold higher than wild-type value | Escherichia coli |
P184A | inactive mutant enzyme | Escherichia coli |
P193A | inactive mutant enzyme | Escherichia coli |
P53A | Km/Vmax for tRNA is 2fold higher than wild-type value | Escherichia coli |
R114A | inactive mutant enzyme | Escherichia coli |
R121A | inactive mutant enzyme | Escherichia coli |
R154A | inactive mutant enzyme | Escherichia coli |
R208A | inactive mutant enzyme | Escherichia coli |
R215A | Km/Vmax for tRNA is 5fold higher than wild-type value | Escherichia coli |
R219A | Km/Vmax for tRNA is 4fold higher than wild-type value | Escherichia coli |
S132A | Km/Vmax for tRNA is 1.5fold higher than wild-type value | Escherichia coli |
V192A | inactive mutant enzyme | Escherichia coli |
W131A | Km/Vmax for tRNA is 1.3fold higher than wild-type value | Escherichia coli |
W207A | inactive mutant enzyme | Escherichia coli |
W207F | Km/Vmax for tRNA is 4fold higher than wild-type value | Escherichia coli |
W207H | Km/Vmax for tRNA is 5.6fold higher than wild-type value | Escherichia coli |
Y136A | Km/Vmax for tRNA is 7.3fold higher than wild-type value | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
62000 | - |
gel filtration | Escherichia coli |
64000 | - |
dynamic light-scattering | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A873 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Subunits | Comment | Organism |
---|---|---|
dimer | each monomer consists of a C-terminal domain connected by a flexible linker to an N-terminal AdoMet-binding domain | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
TrmD | - |
Escherichia coli |
tRNA (guanosine-1) methyltransferase | - |
Escherichia coli |