Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli by using a His-tagged T7 expression vector | Streptococcus pneumoniae |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
S-adenosyl-L-homocysteine | pH 8.5, 37°C | Streptococcus pneumoniae | |
0.0043 | - |
guanine37 in tRNALeu(GAC) | pH 8.5, 37°C | Streptococcus pneumoniae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | divalent cation (in oder of decreasing efficiency: Mg2+, Mn2+, Ca2+) required, optimal activity in presence of 10 mM Mg2+ | Streptococcus pneumoniae | |
Mg2+ | divalent cation (in oder of decreasig efficiency: Mg2+, Mn2+, Ca2+) required, optimal activity in presence of 10 mM Mg2+ | Streptococcus pneumoniae | |
Mn2+ | divalent cation (in oder of decreasing efficiency: Mg2+, Mn2+, Ca2+) required, optimal activity in presence of 10 mM Mg2+ | Streptococcus pneumoniae | |
additional information | no activity is observed in absence of divalent cation or ion presence of Co2+. TrmD activity increases only 10% in the presence of a monovalent cation, indicating the nonessential nature of this factor | Streptococcus pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
2 * 32000 | Streptococcus pneumoniae |
65000 | - |
dynamic light scattering | Streptococcus pneumoniae |
69000 | - |
gel filtration | Streptococcus pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA | Streptococcus pneumoniae | - |
S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptococcus pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Streptococcus pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine37 in tRNA | - |
Streptococcus pneumoniae | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNA | the streptococcal enzyme utilizes a sequential mechanism. Nonsubstrate tRNA species, like tRNAThr(GGT), tRNAPhe, and tRNAAla(TGC), bind the enzyme with similar affinities, suggesting that tRNA specificity is achieved via a postbinding events. The streptococcal TrmD requires the complete tRNA structure since it cannot modify the tRNALeu(CAG) minihelix lacking the D, T, and extra loops of complete tRNA. In addition, and consistent with a requirement for G at positions 36 and 37 in the tRNA, the enzyme methylates yeast tRNAAsp possessing a G36G37 sequence with kinetic values that are indistinguishable from those obtained with substrate tRNALeu(CAG) but does not methylate either tRNAAsp possessing a C36G37 sequence or tRNA(Asp) possessing a C36A37 sequence | Streptococcus pneumoniae | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine37 in tRNALeu(GAC) | - |
Streptococcus pneumoniae | S-adenosyl-L-homocysteine + N1-methylguanine37 in tRNALeu(GAC) | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 32000 | Streptococcus pneumoniae |
Synonyms | Comment | Organism |
---|---|---|
TrmD | - |
Streptococcus pneumoniae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptococcus pneumoniae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.25 | - |
S-adenosyl-L-homocysteine | pH 8.5, 37°C | Streptococcus pneumoniae | |
0.25 | - |
guanine37 in tRNALeu(GAC) | pH 8.5, 37°C | Streptococcus pneumoniae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | 8 | - |
Streptococcus pneumoniae |
8.5 | - |
assay at | Streptococcus pneumoniae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 8.5 | pH 6.0: about 50% of maximal activity, pH 8.5: about 75% of maximal activity | Streptococcus pneumoniae |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
58 | - |
S-adenosyl-L-homocysteine | pH 8.5, 37°C | Streptococcus pneumoniae | |
357 | - |
guanine37 in tRNALeu(GAC) | pH 8.5, 37°C | Streptococcus pneumoniae |