BRENDA - Enzyme Database show
show all sequences of 2.1.1.224

The order Bacillales hosts functional homologs of the worrisome cfr antibiotic resistance gene

Hansen, L.H.; Planellas, M.H.; Long, K.S.; Vester, B.; Antimicrob. Agents Chemother. 56, 3563-3567 (2012)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
gene clbA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10
Bacillus amyloliquefaciens
gene clbB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10
Brevibacillus brevis
gene clbC, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10
Bacillus clausii
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
Bacillus amyloliquefaciens
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
Brevibacillus brevis
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
Bacillus clausii
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
Bacillus amyloliquefaciens FZB42
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
Brevibacillus brevis NBRC 100599
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bacillus amyloliquefaciens
-
gene clbA, a cfr-like gene
-
Bacillus amyloliquefaciens FZB42
-
gene clbA, a cfr-like gene
-
Brevibacillus brevis
C0ZJA6
gene clbB, a cfr-like gene
-
Brevibacillus brevis NBRC 100599
C0ZJA6
gene clbB, a cfr-like gene
-
Bacillus clausii
Q5WJ42
or strain domuvar, gene clbC, a cfr-like gene
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Bacillus amyloliquefaciens
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Brevibacillus brevis
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Bacillus clausii
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Bacillus amyloliquefaciens
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
verification of RNA methylation at A2503 in 23S rRNA by primer extension method
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Brevibacillus brevis
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
verification of RNA methylation at A2503 in 23S rRNA by primer extension method
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Bacillus clausii
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
verification of RNA methylation at A2503 in 23S rRNA by primer extension method
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Bacillus amyloliquefaciens FZB42
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Bacillus amyloliquefaciens FZB42
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
verification of RNA methylation at A2503 in 23S rRNA by primer extension method
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Brevibacillus brevis NBRC 100599
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Brevibacillus brevis NBRC 100599
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
verification of RNA methylation at A2503 in 23S rRNA by primer extension method
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Bacillus amyloliquefaciens
S-adenosyl-L-methionine
-
Bacillus clausii
S-adenosyl-L-methionine
-
Brevibacillus brevis
Cloned(Commentary) (protein specific)
Commentary
Organism
gene clbA, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10
Bacillus amyloliquefaciens
gene clbC, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10
Bacillus clausii
gene clbB, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain AS19 using plasmid pLJ10
Brevibacillus brevis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Bacillus amyloliquefaciens
S-adenosyl-L-methionine
-
Bacillus clausii
S-adenosyl-L-methionine
-
Brevibacillus brevis
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
Bacillus amyloliquefaciens
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
Brevibacillus brevis
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
Bacillus clausii
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
Bacillus amyloliquefaciens FZB42
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
Brevibacillus brevis NBRC 100599
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Bacillus amyloliquefaciens
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Brevibacillus brevis
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Bacillus clausii
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Bacillus amyloliquefaciens
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
verification of RNA methylation at A2503 in 23S rRNA by primer extension method
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Brevibacillus brevis
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
verification of RNA methylation at A2503 in 23S rRNA by primer extension method
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Bacillus clausii
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
verification of RNA methylation at A2503 in 23S rRNA by primer extension method
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Bacillus amyloliquefaciens FZB42
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Bacillus amyloliquefaciens FZB42
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
verification of RNA methylation at A2503 in 23S rRNA by primer extension method
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Brevibacillus brevis NBRC 100599
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718601
Brevibacillus brevis NBRC 100599
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
verification of RNA methylation at A2503 in 23S rRNA by primer extension method
-
-
?
General Information
General Information
Commentary
Organism
evolution
ClbA acts via the same mechanism as the Cfr methyltransferase
Bacillus amyloliquefaciens
evolution
ClbC acts via the same mechanism as the Cfr methyltransferase
Bacillus clausii
evolution
ClbB acts via the same mechanism as the Cfr methyltransferase
Brevibacillus brevis
physiological function
ClbA confers resistance to antibiotics, florfenicol, clindamycin, linezolid, tiamulin, and streptogramin A/streptogramin B, to the cell, also when expressed in Escherichia coli strain AS19, overview
Bacillus amyloliquefaciens
physiological function
ClbC confers resistance to antibiotics, florfenicol, clindamycin, linezolid, tiamulin, and streptogramin A/streptogramin B, to the cell, also when expressed in Escherichia coli strain AS19, overview
Bacillus clausii
physiological function
ClbB confers resistance to antibiotics, florfenicol, clindamycin, linezolid, tiamulin, and streptogramin A/streptogramin B, to the cell, also when expressed in Escherichia coli strain AS19, overview
Brevibacillus brevis
General Information (protein specific)
General Information
Commentary
Organism
evolution
ClbA acts via the same mechanism as the Cfr methyltransferase
Bacillus amyloliquefaciens
evolution
ClbC acts via the same mechanism as the Cfr methyltransferase
Bacillus clausii
evolution
ClbB acts via the same mechanism as the Cfr methyltransferase
Brevibacillus brevis
physiological function
ClbA confers resistance to antibiotics, florfenicol, clindamycin, linezolid, tiamulin, and streptogramin A/streptogramin B, to the cell, also when expressed in Escherichia coli strain AS19, overview
Bacillus amyloliquefaciens
physiological function
ClbC confers resistance to antibiotics, florfenicol, clindamycin, linezolid, tiamulin, and streptogramin A/streptogramin B, to the cell, also when expressed in Escherichia coli strain AS19, overview
Bacillus clausii
physiological function
ClbB confers resistance to antibiotics, florfenicol, clindamycin, linezolid, tiamulin, and streptogramin A/streptogramin B, to the cell, also when expressed in Escherichia coli strain AS19, overview
Brevibacillus brevis
Other publictions for EC 2.1.1.224
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735450
Hansen
A cfr-like gene from Clostridi ...
Clostridioides difficile
Antimicrob. Agents Chemother.
59
5841-5843
2015
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733111
Atkinson
Distinction between the Cfr me ...
Staphylococcus sciuri
Antimicrob. Agents Chemother.
57
4019-4026
2013
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735095
Challand
Cysteine methylation controls ...
Staphylococcus aureus, Staphylococcus sciuri
PLoS ONE
8
e67979
2013
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736280
Wang
-
Quantum chemistry studies of a ...
Staphylococcus aureus
Int. J. Quantum Chem.
113
1409-1415
2013
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718598
Locke
Genetic environment and stabil ...
Staphylococcus aureus, Staphylococcus aureus CM05
Antimicrob. Agents Chemother.
56
332-340
2012
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1
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718601
Hansen
The order Bacillales hosts fun ...
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens FZB42, Bacillus clausii, Brevibacillus brevis, Brevibacillus brevis NBRC 100599
Antimicrob. Agents Chemother.
56
3563-3567
2012
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3
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5
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9
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716783
Yan
RNA methylation by radical SAM ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
108
3930-3934
2011
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716926
Boal
Structural basis for methyl tr ...
Escherichia coli
Science
332
1089-1092
2011
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716927
Grove
A radically different mechanis ...
Escherichia coli
Science
332
604-607
2011
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718587
LaMarre
Low fitness cost of the multid ...
Staphylococcus aureus, Staphylococcus aureus RN4220
Antimicrob. Agents Chemother.
55
3714-3719
2011
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704203
Yan
RlmN and Cfr are radical SAM e ...
Escherichia coli
J. Am. Chem. Soc.
132
3953-3964
2010
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720968
Booth
High-level expression and reco ...
Azotobacter vinelandii
Protein Expr. Purif.
74
204-210
2010
1
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