BRENDA - Enzyme Database
show all sequences of 2.1.1.224

Low fitness cost of the multidrug resistance gene cfr

LaMarre, J.M.; Locke, J.B.; Shaw, K.J.; Mankin, A.S.; Antimicrob. Agents Chemother. 55, 3714-3719 (2011)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
gene gfr, ermB and cfr genes are naturally coexpressed under the Perm promoter in the mlr operon, expression of mlr results in modification of A2058 and A2503 in 23S rRNA and renders cells resistant to all clinically relevant antibiotics that target the large ribosomal subunit
Staphylococcus aureus
Engineering
Protein Variants
Commentary
Organism
C119A
site-directed mutagenesis, the mutant Cfr is inactive, and mutant cells show no resistance against antibiotics
Staphylococcus aureus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Staphylococcus aureus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
Staphylococcus aureus
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
Staphylococcus aureus RN4220
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Staphylococcus aureus
-
gene cfr
-
Staphylococcus aureus RN4220
-
gene cfr
-
Source Tissue
Source Tissue
Commentary
Organism
Textmining
additional information
in some clinical isolates, gene cfr is coexpressed with gene erm, which encodes a methyltransferase targeting another 23S rRNA residue, A2058
Staphylococcus aureus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718587
Staphylococcus aureus
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718587
Staphylococcus aureus RN4220
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
Cfr
-
Staphylococcus aureus
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Staphylococcus aureus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Staphylococcus aureus
Cofactor
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Staphylococcus aureus
Cloned(Commentary) (protein specific)
Commentary
Organism
gene gfr, ermB and cfr genes are naturally coexpressed under the Perm promoter in the mlr operon, expression of mlr results in modification of A2058 and A2503 in 23S rRNA and renders cells resistant to all clinically relevant antibiotics that target the large ribosomal subunit
Staphylococcus aureus
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
S-adenosyl-L-methionine
-
Staphylococcus aureus
Engineering (protein specific)
Protein Variants
Commentary
Organism
C119A
site-directed mutagenesis, the mutant Cfr is inactive, and mutant cells show no resistance against antibiotics
Staphylococcus aureus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Mg2+
required
Staphylococcus aureus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
Staphylococcus aureus
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
Staphylococcus aureus RN4220
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
?
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
additional information
in some clinical isolates, gene cfr is coexpressed with gene erm, which encodes a methyltransferase targeting another 23S rRNA residue, A2058
Staphylococcus aureus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718587
Staphylococcus aureus
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
718587
Staphylococcus aureus RN4220
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Staphylococcus aureus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.5
-
assay at
Staphylococcus aureus
General Information
General Information
Commentary
Organism
additional information
acquisition of the cfr gene does not produce any appreciable reduction in the cell growth rate, analysis of fitness cost of cfr expression, overview. Genes ermB and cfr are coexpressed under the Perm promoter in the mlr operon. Dimethylation of A2058 by the Erm methyltransferase increases the fitness cost associated with Cfr-mediated modification of A2503
Staphylococcus aureus
physiological function
rRNA methyltransferase Cfr that methylates the conserved 23S rRNA residue A2503, located in a functionally critical region of the ribosome, confers resistance to an array of ribosomal antibiotics, including linezolid
Staphylococcus aureus
General Information (protein specific)
General Information
Commentary
Organism
additional information
acquisition of the cfr gene does not produce any appreciable reduction in the cell growth rate, analysis of fitness cost of cfr expression, overview. Genes ermB and cfr are coexpressed under the Perm promoter in the mlr operon. Dimethylation of A2058 by the Erm methyltransferase increases the fitness cost associated with Cfr-mediated modification of A2503
Staphylococcus aureus
physiological function
rRNA methyltransferase Cfr that methylates the conserved 23S rRNA residue A2503, located in a functionally critical region of the ribosome, confers resistance to an array of ribosomal antibiotics, including linezolid
Staphylococcus aureus
Other publictions for EC 2.1.1.224
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
735450
Hansen
A cfr-like gene from Clostridi ...
Clostridioides difficile
Antimicrob. Agents Chemother.
59
5841-5843
2015
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1
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1
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1
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1
1
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733111
Atkinson
Distinction between the Cfr me ...
Staphylococcus sciuri
Antimicrob. Agents Chemother.
57
4019-4026
2013
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1
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1
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1
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1
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2
2
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735095
Challand
Cysteine methylation controls ...
Staphylococcus aureus, Staphylococcus sciuri
PLoS ONE
8
e67979
2013
-
-
2
-
2
-
-
-
-
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2
3
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2
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1
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1
3
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3
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3
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4
1
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-
-
-
-
-
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2
2
-
-
-
736280
Wang
-
Quantum chemistry studies of a ...
Staphylococcus aureus
Int. J. Quantum Chem.
113
1409-1415
2013
-
-
-
-
-
-
-
1
-
-
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2
-
1
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1
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3
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718598
Locke
Genetic environment and stabil ...
Staphylococcus aureus, Staphylococcus aureus CM05
Antimicrob. Agents Chemother.
56
332-340
2012
-
-
1
-
1
-
-
-
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5
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718601
Hansen
The order Bacillales hosts fun ...
Bacillus amyloliquefaciens, Bacillus amyloliquefaciens FZB42, Bacillus clausii, Brevibacillus brevis, Brevibacillus brevis NBRC 100599
Antimicrob. Agents Chemother.
56
3563-3567
2012
-
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3
-
-
-
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5
-
10
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10
-
3
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3
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3
3
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5
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10
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6
6
-
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-
716783
Yan
RNA methylation by radical SAM ...
Escherichia coli
Proc. Natl. Acad. Sci. USA
108
3930-3934
2011
-
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1
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1
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716926
Boal
Structural basis for methyl tr ...
Escherichia coli
Science
332
1089-1092
2011
-
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1
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716927
Grove
A radically different mechanis ...
Escherichia coli
Science
332
604-607
2011
-
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718587
LaMarre
Low fitness cost of the multid ...
Staphylococcus aureus, Staphylococcus aureus RN4220
Antimicrob. Agents Chemother.
55
3714-3719
2011
-
-
1
-
1
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1
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2
-
21
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1
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1
1
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1
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1
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2
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1
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1
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2
2
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704203
Yan
RlmN and Cfr are radical SAM e ...
Escherichia coli
J. Am. Chem. Soc.
132
3953-3964
2010
-
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1
-
3
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1
1
1
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1
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1
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1
1
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720968
Booth
High-level expression and reco ...
Azotobacter vinelandii
Protein Expr. Purif.
74
204-210
2010
1
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1
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1
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1
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3
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1
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1
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1
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1
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1
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705983
Kaminska
Insights into the structure, f ...
Escherichia coli
Nucleic Acids Res.
38
1652-1663
2009
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1
-
13
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1
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2
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1
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13
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1
1
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706726
Giessing
Identification of 8-methyladen ...
Escherichia coli K-12
RNA
15
327-336
2009
-
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4
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1
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1
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701724
Long
The Cfr rRNA methyltransferase ...
Escherichia coli
Antimicrob. Agents Chemother.
50
2500-2505
2006
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7
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1
1
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705766
Kehrenberg
A new mechanism for chloramphe ...
Escherichia coli
Mol. Microbiol.
57
1064-1073
2005
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