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Literature summary for 2.1.1.224 extracted from

  • LaMarre, J.M.; Locke, J.B.; Shaw, K.J.; Mankin, A.S.
    Low fitness cost of the multidrug resistance gene cfr (2011), Antimicrob. Agents Chemother., 55, 3714-3719.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene gfr, ermB and cfr genes are naturally coexpressed under the Perm promoter in the mlr operon, expression of mlr results in modification of A2058 and A2503 in 23S rRNA and renders cells resistant to all clinically relevant antibiotics that target the large ribosomal subunit Staphylococcus aureus

Protein Variants

Protein Variants Comment Organism
C119A site-directed mutagenesis, the mutant Cfr is inactive, and mutant cells show no resistance against antibiotics Staphylococcus aureus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Staphylococcus aureus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Staphylococcus aureus
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA Staphylococcus aureus RN4220
-
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
?

Organism

Organism UniProt Comment Textmining
Staphylococcus aureus
-
gene cfr
-
Staphylococcus aureus RN4220
-
gene cfr
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information in some clinical isolates, gene cfr is coexpressed with gene erm, which encodes a methyltransferase targeting another 23S rRNA residue, A2058 Staphylococcus aureus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
Staphylococcus aureus S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
?
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA
-
Staphylococcus aureus RN4220 S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA
-
?

Synonyms

Synonyms Comment Organism
Cfr
-
Staphylococcus aureus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Staphylococcus aureus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Staphylococcus aureus

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Staphylococcus aureus

General Information

General Information Comment Organism
additional information acquisition of the cfr gene does not produce any appreciable reduction in the cell growth rate, analysis of fitness cost of cfr expression, overview. Genes ermB and cfr are coexpressed under the Perm promoter in the mlr operon. Dimethylation of A2058 by the Erm methyltransferase increases the fitness cost associated with Cfr-mediated modification of A2503 Staphylococcus aureus
physiological function rRNA methyltransferase Cfr that methylates the conserved 23S rRNA residue A2503, located in a functionally critical region of the ribosome, confers resistance to an array of ribosomal antibiotics, including linezolid Staphylococcus aureus