Cloned (Comment) | Organism |
---|---|
gene gfr, ermB and cfr genes are naturally coexpressed under the Perm promoter in the mlr operon, expression of mlr results in modification of A2058 and A2503 in 23S rRNA and renders cells resistant to all clinically relevant antibiotics that target the large ribosomal subunit | Staphylococcus aureus |
Protein Variants | Comment | Organism |
---|---|---|
C119A | site-directed mutagenesis, the mutant Cfr is inactive, and mutant cells show no resistance against antibiotics | Staphylococcus aureus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Staphylococcus aureus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA | Staphylococcus aureus | - |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA | - |
? | |
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA | Staphylococcus aureus RN4220 | - |
S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Staphylococcus aureus | - |
gene cfr | - |
Staphylococcus aureus RN4220 | - |
gene cfr | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | in some clinical isolates, gene cfr is coexpressed with gene erm, which encodes a methyltransferase targeting another 23S rRNA residue, A2058 | Staphylococcus aureus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA | - |
Staphylococcus aureus | S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA | - |
? | |
2 S-adenosyl-L-methionine + adenine2503 in 23S rRNA | - |
Staphylococcus aureus RN4220 | S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 8-methyladenine2503 in 23S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Cfr | - |
Staphylococcus aureus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Staphylococcus aureus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Staphylococcus aureus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Staphylococcus aureus |
General Information | Comment | Organism |
---|---|---|
additional information | acquisition of the cfr gene does not produce any appreciable reduction in the cell growth rate, analysis of fitness cost of cfr expression, overview. Genes ermB and cfr are coexpressed under the Perm promoter in the mlr operon. Dimethylation of A2058 by the Erm methyltransferase increases the fitness cost associated with Cfr-mediated modification of A2503 | Staphylococcus aureus |
physiological function | rRNA methyltransferase Cfr that methylates the conserved 23S rRNA residue A2503, located in a functionally critical region of the ribosome, confers resistance to an array of ribosomal antibiotics, including linezolid | Staphylococcus aureus |