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Literature summary for 2.1.1.223 extracted from

  • Golovina, A.Y.; Sergiev, P.V.; Golovin, A.V.; Serebryakova, M.V.; Demina, I.; Govorun, V.M.; Dontsova, O.A.
    The yfiC gene of E. coli encodes an adenine-N6 methyltransferase that specifically modifies A37 of tRNA1Val(cmo5UAC) (2009), RNA, 15, 1134-1141.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenine37 in tRNA1(Val) Escherichia coli modification of adenine37 in the anticodon loop. The methylation of m6A in tRNA1 Val by YfiC has little influence on the cell growth under standard conditions, the yfiC gene confers a growth advantage under conditions of osmotic and oxidative stress S-adenosyl-L-homocysteine + N6-methyladenine37 in tRNA1(Val)
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 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P31825
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-

Purification (Commentary)

Purification (Comment) Organism
-
 Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenine37 in tRNA1(Val) modification of adenine37 in the anticodon loop. The methylation of m6A in tRNA1 Val by YfiC has little influence on the cell growth under standard conditions, the yfiC gene confers a growth advantage under conditions of osmotic and oxidative stress Escherichia coli S-adenosyl-L-homocysteine + N6-methyladenine37 in tRNA1(Val)
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 ?
S-adenosyl-L-methionine + adenine37 in tRNA1(Val) unmodified tRNA1(Val) with the anticodon cmo5UAC can be methylated by recombinant YfiC protein in vitro. YfiC methyltransferase can methylate tRNA1(Val), but none of other tRNAs tested (tRNA3(Arg), tRNA1(Glu), tRNA2(Val), tRNA5(Leu), tRNA5(Leu)) Escherichia coli S-adenosyl-L-homocysteine + N6-methyladenine37 in tRNA1(Val)
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 ?

Synonyms

Synonyms Comment Organism
YfiC
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 Escherichia coli

General Information

General Information Comment Organism
malfunction inactivation of yfiC gene abolishes m6A formation in tRNA1(Val), while expression of the yfiC gene from a plasmid restores the modification Escherichia coli
physiological function the methylation of m6A in tRNA1(Val) by YfiC has little influence on the cell growth under standard conditions, the yfiC gene confers a growth advantage under conditions of osmotic and oxidative stress Escherichia coli