Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Thermococcus kodakarensis |
expression in Escherichia coli | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine9 in tRNA | Saccharomyces cerevisiae | - |
S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNA | Thermococcus kodakarensis | the bifunctional enzyme catalyzes both methylation of guanine9 and methylation of adenine9 in tRNA | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q12400 | - |
- |
Thermococcus kodakarensis | Q5JD38 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermococcus kodakarensis |
- |
Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine9 in tRNA | - |
Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNA | the bifunctional enzyme catalyzes both methylation of guanine9 and methylation of adenine9 in tRNA | Thermococcus kodakarensis | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNA | formation of N1-methylguanine9 in tRNA(Asp) from Thermococcus kodakaraensis that contains a guanosine at position 9. The enzyme forms approximately the same amount of m1A and m1G when the tRNA of the yeast strain Y16243 is used as substrate. Given that occurrence of A9 and G9 in this tRNA population is almost equal (about 50% each) this result indicates that the enzyme TK0422p does not show any preference for one of these two nucleosides. The ratio m1A/m1G formed from Escherichia coli tRNA is higher than that with tRNA from the yeast Y16243 strain. This is consistent with the fact that there are about two times more tRNAs with A9 than with G9 in Escherichia coli. The enzyme is active in a pH range 5.5-9.75. The intensity of m1A and m1G spots varies greatly as a function of the pH. At pH 5.5, m1A MTase activity of TK0422p is predominant over m1G. At pH 7 or higher, both m1A and m1G are detected, m1G intensity growing with increasing pH | Thermococcus kodakarensis | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA | - |
? | |
S-adenosyl-L-methionine + guanine9 in tRNA | the enzyme is specific for guanine9 in tRNA, does not catalyse methylation of adenine9 in tRNA | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
TK0422p | - |
Thermococcus kodakarensis |
Trm10p (ambiguous) | - |
Thermococcus kodakarensis |
tRNA(m1G9/m1A9)-methyltransferase | - |
Thermococcus kodakarensis |
tRNA(m1G9/m1A9)MTase | - |
Thermococcus kodakarensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
50 | - |
assay at | Thermococcus kodakarensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Thermococcus kodakarensis |
8 | - |
assay at | Saccharomyces cerevisiae |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9.75 | the bifunctional enzyme is active in a pH range 5.5-9.75. The intensity of m1A and m1G spots varies greatly as a function of the pH. At pH 5.5, m1A MTase activity of TK0422p is predominant over m1G. At pH 7 or higher, both m1A and m1G are detected, m1G intensity growing with increasing pH | Thermococcus kodakarensis |