Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Saccharomyces cerevisiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine9 in tRNA | Saccharomyces cerevisiae | TRM10 is required for the catalysis of at least 9 of the 10 m1G modifications observed (tRNA(Trp), tRNA(Pro), tRNA(Val), tRNAi(Met), tRNA(Ile), tRNAICG(Arg), and both tRNAUCU(Arg) species) at position 9 in yeast. It is likely that Trm10p is also responsible for modification of G9 of tRNAAla | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | Q12400 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Saccharomyces cerevisiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine9 in tRNA | TRM10 is required for the catalysis of at least 9 of the 10 m1G modifications observed (tRNA(Trp), tRNA(Pro), tRNA(Val), tRNAi(Met), tRNA(Ile), tRNAICG(Arg), and both tRNAUCU(Arg) species) at position 9 in yeast. It is likely that Trm10p is also responsible for modification of G9 of tRNAAla | Saccharomyces cerevisiae | S-adenosyl-L-homocysteine + N1-methylguanine9 in tRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
tRNA (guanine-N(1)-)-methyltransferase | - |
Saccharomyces cerevisiae |
tRNA m1G9 methyltransferase | - |
Saccharomyces cerevisiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Saccharomyces cerevisiae |
General Information | Comment | Organism |
---|---|---|
malfunction | guanine9 methylation activity is not detectable in trm10-DELTA/trm10-DELTA strain | Saccharomyces cerevisiae |