Protein Variants | Comment | Organism |
---|---|---|
additional information | siRNA knockdown of m1A58 methyltransferase subunits TRM6 or TRM61 | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine58 in tRNA | Homo sapiens | modification at the conserved nucleotide 58 in the TCC loop | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
subunits TRM6 or TRM61 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast cancer cell | - |
Homo sapiens | - |
HEK-293T cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
MCF-10A cell | - |
Homo sapiens | - |
MDA-MB-231 cell | - |
Homo sapiens | - |
neuroblastoma cell | from adrenal gland | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine58 in tRNA | modification at the conserved nucleotide 58 in the TCC loop | Homo sapiens | S-adenosyl-L-homocysteine + N1-methyladenine58 in tRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | two subunit types, TRM6 or TRM61 | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
m1A58-methyltransferase | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | siRNA knockdown of either subunit of the m1A58-methyltransferase results in a slow-growth phenotype, and a marked increase in the amount of m1A58 hypomodified tRNAs. Most m1A58 hypomodified tRNAs can associate with polysomes in varying extents | Homo sapiens |
additional information | microarray method genomic approach to determine the presence of m1A58 hypomodified tRNAs, on the basis of their permissiveness in primer extension, in human cell lines, overview. A58 hypomodification affects stability and involvement of tRNAs in translation. No hypomodification of initiator-tRNAMet. The pattern of the m1A58 hypomodified tRNAs is similar in five human cell lines | Homo sapiens |
physiological function | N1-adenine58 methylation of initiator-tRNAMet is required for stabilizing this tRNA in human cells | Homo sapiens |