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Literature summary for 2.1.1.219 extracted from

  • Roovers, M.; Wouters, J.; Bujnicki, J.M.; Tricot, C.; Stalon, V.; Grosjean, H.; Droogmans, L.
    A primordial RNA modification enzyme: the case of tRNA (m1A) methyltransferase (2004), Nucleic Acids Res., 32, 465-476.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pyrococcus abyssi

Crystallization (Commentary)

Crystallization (Comment) Organism
concentrated by ultrafiltration, crystallisation at 20°C by the sitting-drop vapour diffusion method Pyrococcus abyssi

Protein Variants

Protein Variants Comment Organism
C196S/C233S mutant enzyme C196S/C233S is inactivated at 85°C after 20 min, wild-type enzyme is stable for more than 1 h Pyrococcus abyssi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
30000
-
4 * 30000, SDS-PAGE under reducing conditions Pyrococcus abyssi

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNA Pyrococcus abyssi
-
2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNA
-
?
2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNA Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543
-
2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNA
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus abyssi Q9V1J7
-
-
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 Q9V1J7
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pyrococcus abyssi

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNA
-
Pyrococcus abyssi 2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNA
-
?
2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNA
-
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNA
-
?
2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNAAsp
-
Pyrococcus abyssi 2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNAAsp
-
?
2 S-adenosyl-L-methionine + adenine57/adenine58 in tRNAAsp
-
Pyrococcus abyssi GE5 / CNCM I-1302 / DSM 25543 2 S-adenosyl-L-homocysteine + N1-methyladenine57/N1-methyladenine58 in tRNAAsp
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 30000, SDS-PAGE under reducing conditions Pyrococcus abyssi

Synonyms

Synonyms Comment Organism
TrmI
-
Pyrococcus abyssi
tRNA (m1A) methyltransferase
-
Pyrococcus abyssi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
additional information
-
the stabilisation of Pyrococcus abyssi TrmI at extreme temperatures involves intersubunit disulfide bridges formed between Cys196 and Cys233 that reinforce the tetrameric oligomerisation Pyrococcus abyssi
80
-
1 h, wild-type enzyme and mutant enzyme C196S/C233S are stable Pyrococcus abyssi
85
-
mutant enzyme C196S/C233S is inactivated after 20 min, wild-type enzyme is stable for more than 1 h Pyrococcus abyssi