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Literature summary for 2.1.1.218 extracted from

  • Howell, N.; Jora, M.; Jepson, B.; Limbach, P.; Jackman, J.
    Distinct substrate specificities of the human tRNA methyltransferases TRMT10A and TRMT10B (2019), RNA, 25, 1366-1376 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.218

Protein Variants

Protein Variants Comment Organism
G231R site-directed mutagenesis, the mutation abolishs methylation of tRNAAsp and tRNATrp, with some residual (though reduced) activity on tRNAArg Homo sapiens
G231R/G232R site-directed mutagenesis, the double mutant displays no methylation activity on any of the three substrates Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information single-turnover kinetic analysis of the enzyme with representative tRNA substrates, tRNAArg, tRNATrp, and tRNAAsp Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenine9 in tRNAArg Homo sapiens
-
 S-adenosyl-L-homocysteine + N1-methyladenine9 in tRNAArg
-
 ?
S-adenosyl-L-methionine + adenine9 in tRNAAsp Homo sapiens
-
 S-adenosyl-L-homocysteine + N1-methyladenine9 in tRNAAsp
-
 ?
S-adenosyl-L-methionine + adenine9 in tRNATrp Homo sapiens
-
 S-adenosyl-L-homocysteine + N1-methyladenine9 in tRNATrp
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q6PF06
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity analysis and comparison of hTRMT10A and hTRMT10B enzymes, overview. Purified hTRMT10B lacks m1G9 methyltransferase activity with several tested Saccharomyces cerevisiae and human tRNA substrates. hTRMT10A and hTRMT10B catalyze methylation of different tRNA substrates. hTRMT10B displays only very weak m1G9 activity on two substrates, human tRNAArg and tRNATrp, with no detectible activity on any other G9-containing tRNA. hTRMT10B, and not hTRMT10A, is indeed capable of catalyzing m1A9 methylation on tRNAAsp in vitro, while hTRMT10B has the sole responsibility for generating the m1A9 modification. hTRMT10A displays higher in vitro catalytic rates than hTRMT10B Homo sapiens ?
-
-
S-adenosyl-L-methionine + adenine9 in tRNAArg
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methyladenine9 in tRNAArg
-
 ?
S-adenosyl-L-methionine + adenine9 in tRNAAsp
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methyladenine9 in tRNAAsp
-
 ?
S-adenosyl-L-methionine + adenine9 in tRNATrp
-
 Homo sapiens S-adenosyl-L-homocysteine + N1-methyladenine9 in tRNATrp
-
 ?

Synonyms

Synonyms Comment Organism
hTRMT10B
-
 Homo sapiens
TRMT10B
-
 Homo sapiens
tRNA m1A9 methyltransferase
-
 Homo sapiens
tRNA m1R9 methyltransferase
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Homo sapiens

General Information

General Information Comment Organism
evolution the enzyme belongs to the tRNA m1R9 methyltransferase (Trm10) family, which is conserved throughout eukarya and archaea. Distinct substrate specificities of the human tRNA methyltransferases TRMT10A and TRMT10B. hTRMT10A and hTRMT10B are not biochemically redundant. hTRMT10A is the de facto methyltransferase responsible for all m1G9 formation on cytosolic tRNA, and hTRMT10B has a much more limited and specific role in tRNA processing in humans Homo sapiens
additional information purified hTRMT10B lacks m1G9 methyltransferase activity with several tested Saccharomyces cerevisiae and human tRNA substrates Homo sapiens
physiological function hTRMT10B exhibits a restricted selectivity unusual for the Trm10 enzyme family Homo sapiens