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Literature summary for 2.1.1.216 extracted from

  • Arimbasseri, A.G.; Blewett, N.H.; Iben, J.R.; Lamichhane, T.N.; Cherkasova, V.; Hafner, M.; Maraia, R.J.
    RNA polymerase III output is functionally linked to tRNA dimethyl-G26 modification (2015), PLoS Genet., 11, e1005671.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.216

Activating Compound

Activating Compound Comment Organism Structure
additional information RNA polymerase III mutations associated with hypomyelinating leukodystrophy decrease tRNA transcription, increase of N2,N2-dimethyl G26 modification efficiency and reverse antisuppression Schizosaccharomyces pombe
Rapamycin a decrease in tRNA synthesis by treatment with rapamycin leads to increased N2,N2-dimethyl G26 modification Schizosaccharomyces pombe

Inhibitors

Inhibitors Comment Organism Structure
additional information the efficiency of N2,N2-dimethyl G26 modification on certain tRNAs is decreased in response to maf1-deletion and is associated with antisuppression Schizosaccharomyces pombe

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4 S-adenosyl-L-methionine + guanine26 in tRNA Schizosaccharomyces pombe G26 is modified by N2,N2-dimethylation in many tRNAs by the Trm1 methyltransferase 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
4 S-adenosyl-L-methionine + guanine26 in tRNALeuCAA Schizosaccharomyces pombe
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 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuCAA
-
 ?
4 S-adenosyl-L-methionine + guanine26 in tRNALeuTAG Schizosaccharomyces pombe
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 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuTAG
-
 ?
4 S-adenosyl-L-methionine + guanine26 in tRNASerUCA Schizosaccharomyces pombe
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 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNASerUCA
-
 ?

Organism

Organism UniProt Comment Textmining
Schizosaccharomyces pombe
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4 S-adenosyl-L-methionine + guanine26 in tRNA G26 is modified by N2,N2-dimethylation in many tRNAs by the Trm1 methyltransferase Schizosaccharomyces pombe 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNA
-
 ?
4 S-adenosyl-L-methionine + guanine26 in tRNALeuCAA
-
 Schizosaccharomyces pombe 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuCAA
-
 ?
4 S-adenosyl-L-methionine + guanine26 in tRNALeuTAG
-
 Schizosaccharomyces pombe 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNALeuTAG
-
 ?
4 S-adenosyl-L-methionine + guanine26 in tRNASerUCA
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 Schizosaccharomyces pombe 4 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNASerUCA
-
 ?

Synonyms

Synonyms Comment Organism
Trm1
-
 Schizosaccharomyces pombe
Trm1 methyltransferase
-
 Schizosaccharomyces pombe

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Schizosaccharomyces pombe

General Information

General Information Comment Organism
malfunction little changes occur in the relative levels of different tRNAs in maf1DELTA cells. By contrast, the efficiency of N2,N2-dimethyl G26 modification on certain tRNAs is decreased in response to maf1-deletion and is associated with antisuppression Schizosaccharomyces pombe
metabolism global tRNA gene activation occurs with derepression of RNA polymerase III via maf1-deletion and is accompanied by a paradoxical loss of tRNA-mediated nonsense suppressor activity, manifested as an antisuppression phenotype. maf1-Antisuppression also occurs in the fission yeast amidst general activation of RNA polymerase III. Dimethyl-guanine-26 varies in the efficiency by which it is added to its target tRNAs, in a manner that is dependent on the overall activity rate of RNA polyymerase III. Overexpression of Trm1, which produces the N2,N2-dimethyl G26 modification, reverses maf1-antisuppression. Competition by increased tRNA levels in maf1DELTA cells leads to N2,N2-dimethyl G26 hypomodification due to limiting Trm1, reducing the activity of suppressor-tRNASerUCA and accounting for antisuppression Schizosaccharomyces pombe
physiological function the N2,N2-dimethyl G26 modification activates the tRNA for function to translate the code. The N2,N2-dimethyl G26 modification is required for the suppressor activity of sup-tRNASerUCA. The enzyme activity is regulated by nutrient/growth conditions Schizosaccharomyces pombe