Literature summary for 2.1.1.213 extracted from

Urbonavicius, J.; Armengaud, J.; Grosjean, H.
Identity elements required for enzymatic formation of N2,N2-dimethylguanosine from N2-monomethylated derivative and its possible role in avoiding alternative conformations in archaeal tRNA (2006), J. Mol. Biol., 357, 387-399.

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Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	yield of G10 dimethylation in transcript of tRNAAsp greatly depends on the presence of Mg2+, with an optimal concentration around 5 mM. In contrast, formation of N2-monomethylguanine10 is not strictly dependent on the presence of Mg2+	Pyrococcus abyssi

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus abyssi	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 S-adenosyl-L-methionine + guanine10 in tRNA	identity elements required for the efficient formation of N2-dimethylguanine10: uracil25 and a V-loop of four nucleotides are major determinants for the formation of N2-dimethylguanine10, whereas the identity of the 13-22 base-pair clearly influences the rate of N2-dimethylguanine10 formation. The integrity of the T-arm prolonged with the amino acid acceptor stem is an important structural element for the dimethylation step catalysed by PabTrm-m2 2G10 enzyme	Pyrococcus abyssi	2 S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA	-	?
S-adenosyl-L-methionine + guanine10 in tRNA	-	Pyrococcus abyssi	S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA	-	?

Synonyms

Synonyms	Comment	Organism
PabTrm-m2 2G10 enzyme	-	Pyrococcus abyssi

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Release 2023.1 (January 2023)