Literature summary for 2.1.1.213 extracted from

Armengaud, J.; Urbonavicius, J.; Fernandez, B.; Chaussinand, G.; Bujnicki, J.M.; Grosjean, H.
N2-Methylation of guanosine at position 10 in tRNA is catalyzed by a THUMP domain-containing, S-adenosylmethionine-dependent methyltransferase, conserved in archaea and eukaryota (2004), J. Biol. Chem., 279, 37142-37152.

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Cloned(Commentary)

Cloned (Comment)	Organism
overexpression in Escherichia coli	Pyrococcus abyssi

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
39000	-	1 * 39000, SDS-PAGE	Pyrococcus abyssi

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2 S-adenosyl-L-methionine + guanine10 in tRNA	Pyrococcus abyssi	depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures	2 S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA	-	?
S-adenosyl-L-methionine + guanine10 in tRNA	Pyrococcus abyssi	depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures	S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Pyrococcus abyssi	Q9UY84	strain GE5	-

Purification (Commentary)

Purification (Comment)	Organism
-	Pyrococcus abyssi

Reaction

Reaction	Comment	Organism	Reaction ID
S-adenosyl-L-methionine + guanine10 in tRNA = S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA	(1a)	Pyrococcus abyssi	a
S-adenosyl-L-methionine + N2-methylguanine10 in tRNA = S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA	(1b)	Pyrococcus abyssi	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2 S-adenosyl-L-methionine + guanine10 in tRNA	depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures	Pyrococcus abyssi	2 S-adenosyl-L-homocysteine + N2-dimethylguanine10 in tRNA	-	?
S-adenosyl-L-methionine + guanine10 in tRNA	depending on the experimental conditions used, as well as the tRNA substrate tested, the enzymatic reaction leads to the formation of either N2-methyl or N2-dimethylguanosine. N2-Dimethylguanine10 accumulates during incubation at 50°C, whereas the formation of N2-methylguanine10 appears to be a transient intermediate. At 30°C, N2-dimethylguanine10 is efficiently formed, whereas the formation of N2-methylguanine10 is considerably slowed compared with that at higher temperatures	Pyrococcus abyssi	S-adenosyl-L-homocysteine + N2-methylguanine10 in tRNA	-	?

Subunits

Subunits	Comment	Organism
monomer	1 * 39000, SDS-PAGE	Pyrococcus abyssi

Synonyms

Synonyms	Comment	Organism
N(2),N(2)-dimethylguanosine tRNA methyltransferase	-	Pyrococcus abyssi
PAB1283	-	Pyrococcus abyssi
PabTrm-G10	-	Pyrococcus abyssi
Trm-G10	-	Pyrococcus abyssi

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
50	-	assay at	Pyrococcus abyssi

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
86	-	noticeable unfolding of the protein (more than 5%) is detected above 86°C	Pyrococcus abyssi
102	-	midpoint melting temperature	Pyrococcus abyssi

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.2	-	assay at	Pyrococcus abyssi

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Release 2023.1 (January 2023)