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Literature summary for 2.1.1.208 extracted from

  • Mosbacher, T.G.; Bechthold, A.; Schulz, G.E.
    Structure and function of the antibiotic resistance-mediating methyltransferase AviRb from Streptomyces viridochromogenes (2005), J. Mol. Biol., 345, 535-545.
    View publication on PubMed
Search for more literature for 2.1.1.208

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli as an N-terminal GST-fusion protein that is subsequently cleaved with thrombin Streptomyces viridochromogenes

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion method at 20°C. Crystals obtained belong to space group P4(1)2(1)2 and contained two subunits of AviRb per crystallographic asymmetric unit Streptomyces viridochromogenes

Protein Variants

Protein Variants Comment Organism
E234A 20% of wild-type activity Streptomyces viridochromogenes
E234Q 70% of wild-type activity Streptomyces viridochromogenes
N139A less than 5% of wild-type activity Streptomyces viridochromogenes
N262A less than 5% of wild-type activity Streptomyces viridochromogenes
N262Q 60% of wild-type activity Streptomyces viridochromogenes
R145A less than 5% of wild-type activity Streptomyces viridochromogenes
T135V 110% of wild-type activity Streptomyces viridochromogenes

General Stability

General Stability Organism
AviRb solutions with concentrations above about 0.5 mg/ml are unstable at ionic strengths below 0.3 Streptomyces viridochromogenes

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
31602
-
 2 * 31602, calculated from sequence Streptomyces viridochromogenes
60000
-
 dynamic light scattering Streptomyces viridochromogenes
67000
-
 gel filtration Streptomyces viridochromogenes

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + uridine2479 in 23S rRNA Streptomyces viridochromogenes AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of uridine2479 of the 23S ribosomal RNA in Gram-positive bacteria and thus mediates resistance to the oligosaccharide antibiotic avilamycin S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Streptomyces viridochromogenes Q9F5K6
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Streptomyces viridochromogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + uridine2479 in 23S rRNA
-
 Streptomyces viridochromogenes S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + uridine2479 in 23S rRNA AviRb from Streptomyces viridochromogenes methylates the 2'-O atom of uridine2479 of the 23S ribosomal RNA in Gram-positive bacteria and thus mediates resistance to the oligosaccharide antibiotic avilamycin Streptomyces viridochromogenes S-adenosyl-L-homocysteine + 2'-O-methyluridine2479 in 23S rRNA
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 31602, calculated from sequence Streptomyces viridochromogenes

Synonyms

Synonyms Comment Organism
AviRb
-
 Streptomyces viridochromogenes