Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.204 extracted from

  • Jurkowski, T.P.; Shanmugam, R.; Helm, M.; Jeltsch, A.
    Mapping the tRNA binding site on the surface of human DNMT2 methyltransferase (2012), Biochemistry, 51, 4438-4444.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
phylogenetic analysis, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
C79A site-directed mutagenesis, inactive mutant Homo sapiens
E119A site-directed mutagenesis, inactive mutant Homo sapiens
K122A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Homo sapiens
K168A site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme Homo sapiens
K196A site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme Homo sapiens
K241A site-directed mutagenesis, the mutant shows moderately reduced activity compared to the wild-type enzyme Homo sapiens
K251A site-directed mutagenesis, the mutant shows moderately reduced activity compared to the wild-type enzyme Homo sapiens
K254A site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme Homo sapiens
K271A site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme Homo sapiens
K295A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Homo sapiens
K346A site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme Homo sapiens
K363A site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme Homo sapiens
K367A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Homo sapiens
K387A site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme Homo sapiens
R160A site-directed mutagenesis, inactive mutant Homo sapiens
R162A site-directed mutagenesis, inactive mutant Homo sapiens
R240A site-directed mutagenesis, the mutant shows only slightly reduced activity compared to the wild-type enzyme Homo sapiens
R275A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Homo sapiens
R288A site-directed mutagenesis, the mutant shows moderately reduced activity compared to the wild-type enzyme Homo sapiens
R289A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Homo sapiens
R369A site-directed mutagenesis, the mutant shows moderately reduced activity compared to the wild-type enzyme Homo sapiens
R371A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Homo sapiens
R84A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Homo sapiens
R95A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + cytosine38 in tRNAAsp Homo sapiens
-
S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli to over 90% purity, except for mutant R84A, which is about 75% pure Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information DNMT2 induces a conformational change in the tRNA of the DNMT2-tRNA complex Homo sapiens ?
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp
-
Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
?
S-adenosyl-L-methionine + cytosine38 in tRNAAsp mapping of the tRNA binding site of DNMT2 by systematically mutating surface-exposed lysine and arginine residues to alanine and studying the tRNA methylation activity and binding of the corresponding variants. tRNA specificity determinants and tRNA binding pocket structure in the DNMT2, overview Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNAAsp
-
?

Subunits

Subunits Comment Organism
More structural modeling of DNMT2, overview Homo sapiens

Synonyms

Synonyms Comment Organism
DNMT2 methyltransferase
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
Homo sapiens

General Information

General Information Comment Organism
evolution DNMT2 methylates RNA by employing a DNA methyltransferase-like catalytic mechanism, which is clearly different from the mechanism of other RNA MTases. DNMT2 has changed its substrate specificity from DNA to RNA in the course of its evolution Homo sapiens