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Literature summary for 2.1.1.204 extracted from

  • Jurkowski, T.P.; Meusburger, M.; Phalke, S.; Helm, M.; Nellen, W.; Reuter, G.; Jeltsch, A.
    Human DNMT2 methylates tRNA(Asp) molecules using a DNA methyltransferase-like catalytic mechanism (2008), RNA, 14, 1663-1670.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.204

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
C292A about 3fold reduction in RNA binding affinity. Significant residual activity Homo sapiens
C79A about 3fold reduction in RNA binding affinity. No detectable in vitro methylation activity Homo sapiens
E119A mutant binds stronger to RNA than wild-type. No detectable in vitro methylation activity Homo sapiens
R160A no detectable in vitro methylation activity Homo sapiens
R162A no detectable in vitro methylation activity Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ supports native folding of the substrate tRNA Homo sapiens

Organism

Organism UniProt Comment Textmining
Drosophila melanogaster
-
-
-
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + cytosine38 in tRNA in vitro transcribed tRNAAsp is methylated by DNMT2, albeit at reduced efficiency. Human, mouse, and Dictyostelium tRNA all are substrates for human DNMT2. C79, E119, R160 and R162 are essential for the catalytic mechanism of DNMT2 Homo sapiens S-adenosyl-L-homocysteine + 5-methylcytosine38 in tRNA
-
 ?

Synonyms

Synonyms Comment Organism
Dnmt2
-
 Drosophila melanogaster
Dnmt2
-
 Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Homo sapiens