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Literature summary for 2.1.1.20 extracted from

  • Luka, Z.
    Methyltetrahydrofolate in folate-binding protein glycine N-methyltransferase (2008), Vitam. Horm., 79, 325-345.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Homo sapiens
-
Rattus norvegicus
-
Oryctolagus cuniculus
-
Mus musculus
-
Sus scrofa

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
H176N the mutant activity decreases 25% regarding Vmax Homo sapiens
L149P the mutant is inactivated by 90% Homo sapiens
N140S the mutant possesses only traces of wild type activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
5-methyl-tetrahydrofolate
-
Homo sapiens
5-methyl-tetrahydrofolate
-
Mus musculus
5-methyl-tetrahydrofolate
-
Oryctolagus cuniculus
5-methyl-tetrahydrofolate
-
Rattus norvegicus
5-methyl-tetrahydrofolate
-
Sus scrofa
5-Methyltetrahydrofolate pentaglutamate noncompetitive inhibitor with respect to substrates S-adenosyl-L-methionine and glycine Homo sapiens
5-Methyltetrahydrofolate pentaglutamate noncompetitive inhibitor regarding substrates S-adenosyl-L-methionine and glycine Mus musculus
5-Methyltetrahydrofolate pentaglutamate noncompetitive inhibitor with respect to substrates S-adenosyl-L-methionine and glycine Oryctolagus cuniculus
5-Methyltetrahydrofolate pentaglutamate noncompetitive inhibitor with respect to substrates S-adenosyl-L-methionine and glycine Rattus norvegicus
5-Methyltetrahydrofolate pentaglutamate noncompetitive inhibitor with respect to substrates S-adenosyl-L-methionine and glycine Sus scrofa

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
Homo sapiens 5829
-
cytosol
-
Rattus norvegicus 5829
-
cytosol
-
Oryctolagus cuniculus 5829
-
cytosol
-
Mus musculus 5829
-
cytosol
-
Sus scrofa 5829
-
nucleus
-
Homo sapiens 5634
-
nucleus
-
Rattus norvegicus 5634
-
nucleus
-
Oryctolagus cuniculus 5634
-
nucleus
-
Mus musculus 5634
-
nucleus
-
Sus scrofa 5634
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32500
-
4 * 32500 Homo sapiens
32500
-
4 * 32500 Rattus norvegicus
32500
-
4 * 32500 Oryctolagus cuniculus
32500
-
4 * 32500 Mus musculus
32500
-
4 * 32500 Sus scrofa
130000
-
-
Homo sapiens
130000
-
-
Rattus norvegicus
130000
-
-
Oryctolagus cuniculus
130000
-
-
Mus musculus
130000
-
-
Sus scrofa

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus Q9QXF8
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-
Sus scrofa Q29555
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein GNMT is phosphorylated by cAMP-dependent protein kinase at Ser9, Ser71, Ser139, Ser182, and Ser241 Homo sapiens
phosphoprotein GNMT is phosphorylated by cAMP-dependent protein kinase at Ser9, Ser71, Ser139, Ser182, and Ser241 Rattus norvegicus
phosphoprotein GNMT is phosphorylated by cAMP-dependent protein kinase at Ser9, Ser71, Ser139, Ser182, and Ser241 Oryctolagus cuniculus
phosphoprotein GNMT is phosphorylated by cAMP-dependent protein kinase at Ser9, Ser71, Ser139, Ser182, and Ser241 Sus scrofa

Purification (Commentary)

Purification (Comment) Organism
-
Homo sapiens
-
Rattus norvegicus
-
Oryctolagus cuniculus
-
Mus musculus
-
Sus scrofa

Source Tissue

Source Tissue Comment Organism Textmining
hepatocellular carcinoma cell
-
Homo sapiens
-
hepatocellular carcinoma cell
-
Mus musculus
-
kidney
-
Homo sapiens
-
kidney
-
Rattus norvegicus
-
kidney
-
Mus musculus
-
kidney
-
Sus scrofa
-
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-
liver
-
Oryctolagus cuniculus
-
liver
-
Mus musculus
-
liver
-
Sus scrofa
-
additional information no GNMT activity is detected in fetal liver, Novikoff hepatoma cells, Morris hepatoma cells, and Ehrlich ascites cells Homo sapiens
-
additional information no GNMT activity is detected in fetal liver, Novikoff hepatoma cells, Morris hepatoma cells, and Ehrlich ascites cells Mus musculus
-
pancreas
-
Homo sapiens
-
pancreas
-
Rattus norvegicus
-
pancreas
-
Mus musculus
-
pancreas
-
Sus scrofa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information GNMT is phosphorylated by cAMP-dependent protein kinase at Ser9, Ser71, Ser139, Ser182, and Ser241 Mus musculus ?
-
?
S-adenosyl-L-methionine + glycine
-
Homo sapiens S-adenosyl-L-homocysteine + N-methylglycine
-
?
S-adenosyl-L-methionine + glycine
-
Rattus norvegicus S-adenosyl-L-homocysteine + N-methylglycine
-
?
S-adenosyl-L-methionine + glycine
-
Oryctolagus cuniculus S-adenosyl-L-homocysteine + N-methylglycine
-
?
S-adenosyl-L-methionine + glycine
-
Mus musculus S-adenosyl-L-homocysteine + N-methylglycine
-
?
S-adenosyl-L-methionine + glycine
-
Sus scrofa S-adenosyl-L-homocysteine + N-methylglycine
-
?

Subunits

Subunits Comment Organism
homotetramer 4 * 32500 Homo sapiens
homotetramer 4 * 32500 Rattus norvegicus
homotetramer 4 * 32500 Oryctolagus cuniculus
homotetramer 4 * 32500 Mus musculus
homotetramer 4 * 32500 Sus scrofa

Synonyms

Synonyms Comment Organism
GNMT
-
Homo sapiens
GNMT
-
Rattus norvegicus
GNMT
-
Oryctolagus cuniculus
GNMT
-
Mus musculus
GNMT
-
Sus scrofa

pI Value

Organism Comment pI Value Maximum pI Value
Rattus norvegicus
-
-
6.4

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
0.001
-
at pH 9.0 Rattus norvegicus 5-Methyltetrahydrofolate pentaglutamate
0.002
-
at pH 7.5 Rattus norvegicus 5-Methyltetrahydrofolate pentaglutamate