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Literature summary for 2.1.1.20 extracted from

  • Luka, Z.; Loukachevitch, L.V.; Wagner, C.
    Acetylation of N-terminal valine of glycine N-methyltransferase affects enzyme inhibition by folate (2008), Biochim. Biophys. Acta, 1784, 1342-1346.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.20

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
5-methyltetrahydrofolate monoglutamate native N-acetylated form of the enzyme shows 50% inhibition at 0.05 mM while the recombinant non-acetylated form shows 50% inhibition at 1.89 mM Rattus norvegicus
5-Methyltetrahydrofolate pentaglutamate native N-acetylated form of the enzyme shows 50% inhibition at 0.0013 mM while the recombinant non-acetylated form shows 50% inhibition at 0.59 mM Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 glycine pH 7.5, 25°C, recombinant enzyme Rattus norvegicus
1.9
-
 glycine pH 7.5, 25°C, native enzyme Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
130000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus major folate binding protein ?
-
 ?
S-adenosyl-L-methionine + glycine Rattus norvegicus key enzyme for the regulation of the ratio of S-adenosylmethionine to S-adenosylhomocysteine S-adenosyl-L-homocysteine + N-methylglycine N-methylglycine = sarcosine ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
acetylated protein N-terminal valine of native GNMT is N-acetylated while in the recombinant enzyme it is not Rattus norvegicus

Purification (Commentary)

Purification (Comment) Organism
native enzyme from rat liver and recombinant enzyme from Escherichia coli Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information major folate binding protein Rattus norvegicus ?
-
 ?
S-adenosyl-L-methionine + glycine
-
 Rattus norvegicus S-adenosyl-L-homocysteine + N-methylglycine N-methylglycine = sarcosine ?
S-adenosyl-L-methionine + glycine key enzyme for the regulation of the ratio of S-adenosylmethionine to S-adenosylhomocysteine Rattus norvegicus S-adenosyl-L-homocysteine + N-methylglycine N-methylglycine = sarcosine ?

Synonyms

Synonyms Comment Organism
GNMT
-
 Rattus norvegicus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay temperature Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.7
-
 glycine pH 7.5, 25°C, native enzyme Rattus norvegicus
51.9
-
 glycine pH 7.5, 25°C, recombinant enzyme Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay pH Rattus norvegicus