Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) | Rattus norvegicus |
expressed in Escherichia coli BL21(GE3) | Rattus norvegicus |
into pET-17b vector and expressed in Escherichia coli BL21(GE3) | Rattus norvegicus |
Crystallization (Comment) | Organism |
---|---|
crystallized by the sitting drop method in complex with (6S)-5-methyltetrahydrofolat monoglutamate | Rattus norvegicus |
GNMT complexed with 5-methyltetrahydrofolate, by the sitting drop method at room temperature, two folate binding sites in the intersubunit areas of the tetramer, each folate binding site is formed primarily by two 1-7 N-terminal regions of one pair of subunits and two 205-218 regions of the other pair of subunits. Both the pteridine and p-aminobenzoyl rings are located in the hydrophobic cavities formed by Tyr5, Leu207, and Met215 residues of all subunits | Rattus norvegicus |
sitting-drop vapor diffusion method in complex with 5-methyltetrahydrofolate pentaglutamate, two molecules of inhibitor bound to a tetramer | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5-methyltetrahydrofolate | - |
Rattus norvegicus | |
5-Methyltetrahydrofolate pentaglutamate | - |
Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytoplasm | - |
Rattus norvegicus | 5737 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | major folate binding protein | ? | - |
? | |
S-adenosyl-L-methionine + glycine | Rattus norvegicus | key regulatory enzyme for methyl group metabolism by regulating the S-adenosyl-L-methionine/S-adenosyl-L-homocysteine ratio | S-adenosyl-L-homocysteine + N-methylglycine | - |
? | |
S-adenosyl-L-methionine + glycine | Rattus norvegicus | key enzyme for the regulation of the ratio of S-adenosylmethionine to S-adenosylhomocysteine, control of the methylating potential of the cell | S-adenosyl-L-homocysteine + N-methylglycine | N-methylglycine = sarcosine | ? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | P13255 | - |
- |
Purification (Comment) | Organism |
---|---|
by ammonium sulfate precipitation and gel filtration | Rattus norvegicus |
recombinant protein | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | major folate binding protein | Rattus norvegicus | ? | - |
? | |
S-adenosyl-L-methionine + glycine | key regulatory enzyme for methyl group metabolism by regulating the S-adenosyl-L-methionine/S-adenosyl-L-homocysteine ratio | Rattus norvegicus | S-adenosyl-L-homocysteine + N-methylglycine | - |
? | |
S-adenosyl-L-methionine + glycine | key enzyme for the regulation of the ratio of S-adenosylmethionine to S-adenosylhomocysteine, control of the methylating potential of the cell | Rattus norvegicus | S-adenosyl-L-homocysteine + N-methylglycine | N-methylglycine = sarcosine | ? |
Subunits | Comment | Organism |
---|---|---|
tetramer | crystal structure analysis | Rattus norvegicus |
tetramer | crystallographic studies | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
GNMT | - |
Rattus norvegicus |