Cloned (Comment) | Organism |
---|---|
the open reading frame of the guanidinoacetate N-methyltransferase gene (NM_000156) from a fibroblast cell line is transfected into primary fibroblasts from a guanidinoacetate N-methyltransferase-deficient patient (Trp20Ser) or transient transected in HeLa cells. | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60000 | - |
Western Blot analyses shows the presences of the guanidinoacetate N-methyltransferase-EGFP fusion protein | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q14353 | fibroblast cell line | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fibroblast cell line | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
stable transfection of primary guanidinoacetate N-methyltransferase-deficient fibroblasts with pEGFP-guanidinoacetate N-methyltransferase results in the restoration of guanidinoacetate N-methyltransferase activity to 501 pmol creatine/mg/h from original 1 pmol creatine/mg/h. A 56fold increased guanidinoacetate N-methyltransferase activity is detected in transfected HeLa cells (7700 pmol creatine/mg/h vs. original 137 pmol creatine/mg/h). | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
GAMT | - |
Homo sapiens |