Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) Star cells | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
in complex with S-adenosyl-L-methionine, sitting drop vapor diffusion method, using 0.2 M DL-malic acid pH 7.0, 20% (w/v) PEG 3350 | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography, heparin column chromatography, and Superdex 200 gel filtration | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytidine1402 in 16S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methylcytidine1402 in 16S rRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
RsmI | - |
Escherichia coli |