Cloned (Comment) | Organism |
---|---|
cloned as a N-terminal His-tag fusion protein, which allows easy purification, purified recombinant YggJ specifically methylates m3U1498 in vitro | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
29000 | - |
x * 29000, SDS-PAGE | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uracil1498 in 16S rRNA | Escherichia coli | - |
S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0AGL7 | the gene is well-conserved across bacteria and plants | - |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + uracil1498 in 16S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + uracil1498 in 16S rRNA | the enzyme is highly specific for U1498 in 16S rRNA. The purified enzyme is able to incorporate methyl groups from S-adenosyl-L-methionine into 30S particles purified from the mutant strain but not into 30S particles purified from wild type. Approximately one methyl group could be incorporated per mutant 30S subunit at high levels of enzyme | Escherichia coli | S-adenosyl-L-homocysteine + N3-methyluracil1498 in 16S rRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 29000, SDS-PAGE | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
DUF558 protein | - |
Escherichia coli |
m3U1498 specific methyltransferase | - |
Escherichia coli |
RsmE | - |
Escherichia coli |
YggJ | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | a yggJ deletion strain lacks the methyl group at U1498 as well as the specific methyltransferase activity. The deletion strain is unaffected in exponential growth in rich or minimal media at multiple temperatures, but it is defective when grown in competition with isogenic wild-type cells | Escherichia coli |