Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.1.1.192 extracted from

  • Silakov, A.; Grove, T.L.; Radle, M.I.; Bauerle, M.R.; Green, M.T.; Rosenzweig, A.C.; Boal, A.K.; Booker, S.J.
    Characterization of a cross-linked protein-nucleic acid substrate radical in the reaction catalyzed by RlmN (2014), J. Am. Chem. Soc., 136, 8221-8228.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.192

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant C118A, hanging drop vapor diffusion method, using 7.5% (w/v) PEG 6000, 0.1 M HEPES, pH 7.5, and 5% (v/v) 2-methyl-2,4-pentanediol as the precipitant Escherichia coli

Protein Variants

Protein Variants Comment Organism
C118A inactive Escherichia coli
C118S inactive Escherichia coli
E105A the mutant shows wild type activity Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + adenine2503 in 23S rRNA Escherichia coli the enzyme only catalyzes C2 methylation of adenine2503 in 23S rRNA S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + adenine2503 in 23S rRNA the enzyme only catalyzes C2 methylation of adenine2503 in 23S rRNA Escherichia coli S-adenosyl-L-homocysteine + L-methionine + 5'-deoxyadenosine + 2-methyladenine2503 in 23S rRNA
-
 ?

Synonyms

Synonyms Comment Organism
RlmN
-
 Escherichia coli

Cofactor

Cofactor Comment Organism Structure
[4Fe-4S]-center
-
 Escherichia coli