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Literature summary for 2.1.1.189 extracted from

  • Auxilien, S.; Rasmussen, A.; Rose, S.; Brochier-Armanet, C.; Husson, C.; Fourmy, D.; Grosjean, H.; Douthwaite, S.
    Specificity shifts in the rRNA and tRNA nucleotide targets of archaeal and bacterial m5U methyltransferases (2011), RNA, 17, 45-53.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.189

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + uracil747 in 23S rRNA Escherichia coli
-
 S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + uracil747 in 23S rRNA Pyrococcus abyssi
-
 S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
 gene rlmC
-
Pyrococcus abyssi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information substrate specificity, overview Pyrococcus abyssi ?
-
 ?
S-adenosyl-L-methionine + uracil747 in 23S rRNA
-
 Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + uracil747 in 23S rRNA
-
 Pyrococcus abyssi S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
 ?
S-adenosyl-L-methionine + uracil747 in 23S rRNA PAB0760 is a m5U methyltransferase and recognizes a specific uridine within the loop of 23S rRNA hairpin 35 in a highly specific manner Pyrococcus abyssi S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA product identification by reverse transcriptase primer extension and MALDI mass spectrometry ?
S-adenosyl-L-methionine + uracil747 in 23S rRNA RlmC recognizes a specific uridine within the loop of 23S rRNA hairpin 35 Escherichia coli S-adenosyl-L-homocysteine + 5-methyluracil747 in 23S rRNA
-
 ?

Synonyms

Synonyms Comment Organism
m5U methyltransferase
-
 Escherichia coli
m5U methyltransferase
-
 Pyrococcus abyssi
PAB0760
-
 Pyrococcus abyssi
RlmC
-
 Escherichia coli
RumB formerly Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
-
 Pyrococcus abyssi

General Information

General Information Comment Organism
evolution PAB0760 originates as RlmD-type m5U methyltransferase and undergoes changes in target specificity after its acquisition by a Thermococcales ancestor from a bacterial source. PAB0760 possesses bacterial RlmC-like activity and specifically methylates the nucleotide equivalent to U747 in Pyrococcus abyssi 23S rRNA, but with a sequence most closely related to the bacterial RlmD, the archetypical enzyme that is specific for m5U1939 in 23S rRNA Pyrococcus abyssi