Cloned (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
- |
Streptococcus pneumoniae |
- |
Corynebacterium glutamicum |
- |
Micromonospora griseorubida |
- |
Streptomyces fradiae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Priestia megaterium | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Bacillus megaterium shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Enterococcus faecalis | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterococcus faecalis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Lactiplantibacillus plantarum | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactobacillus plantarum shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Lactococcus lactis | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactococcus lactis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Rhodococcus corynebacterioides | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Nocardia corynebacteroides shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Streptococcus thermophilus | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Streptococcus thermophilus shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Bacillus subtilis | Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Streptococcus pneumoniae | Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Corynebacterium glutamicum | Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Micromonospora griseorubida | Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Streptomyces fradiae | Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Corynebacterium glutamicum | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Enterococcus faecalis | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Lactiplantibacillus plantarum | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Lactococcus lactis | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Micromonospora griseorubida | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Priestia megaterium | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Rhodococcus corynebacterioides | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Streptococcus pneumoniae | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Streptococcus thermophilus | - |
comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Streptomyces fradiae | Q9S1M6 | comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
- |
Streptococcus pneumoniae |
- |
Corynebacterium glutamicum |
- |
Micromonospora griseorubida |
- |
Streptomyces fradiae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Bacillus megaterium shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro | Priestia megaterium | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterococcus faecalis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro | Enterococcus faecalis | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactobacillus plantarum shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro | Lactiplantibacillus plantarum | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Lactococcus lactis shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro | Lactococcus lactis | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Nocardia corynebacteroides shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro | Rhodococcus corynebacterioides | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Streptococcus thermophilus shows intrinsic 23S rRNA methylation at G748. No methylation is determined with the recombinant protein in vivo or in vitro | Streptococcus thermophilus | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | Bacillus subtilis | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | Streptococcus pneumoniae | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | Corynebacterium glutamicum | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | Micromonospora griseorubida | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine748 in 23S rRNA | Gram-positive 23S rRNAs are methylated at G748. 23S rRNA of Gram-negatives is methylated at G745. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate | Streptomyces fradiae | S-adenosyl-L-homocysteine + N1-methylguanine748 in 23S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RlmAII | - |
Lactiplantibacillus plantarum |
RlmAII | - |
Bacillus subtilis |
RlmAII | - |
Priestia megaterium |
RlmAII | - |
Lactococcus lactis |
RlmAII | - |
Enterococcus faecalis |
RlmAII | - |
Streptococcus pneumoniae |
RlmAII | - |
Streptococcus thermophilus |
RlmAII | - |
Micromonospora griseorubida |
RlmAII | - |
Rhodococcus corynebacterioides |
RlmAII | - |
Streptomyces fradiae |
rRNA large subunit methyltransferase II | - |
Lactiplantibacillus plantarum |
rRNA large subunit methyltransferase II | - |
Bacillus subtilis |
rRNA large subunit methyltransferase II | - |
Priestia megaterium |
rRNA large subunit methyltransferase II | - |
Lactococcus lactis |
rRNA large subunit methyltransferase II | - |
Enterococcus faecalis |
rRNA large subunit methyltransferase II | - |
Streptococcus pneumoniae |
rRNA large subunit methyltransferase II | - |
Streptococcus thermophilus |
rRNA large subunit methyltransferase II | - |
Micromonospora griseorubida |
rRNA large subunit methyltransferase II | - |
Rhodococcus corynebacterioides |
rRNA large subunit methyltransferase II | - |
Streptomyces fradiae |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Lactiplantibacillus plantarum |
37 | - |
assay at | Bacillus subtilis |
37 | - |
assay at | Priestia megaterium |
37 | - |
assay at | Lactococcus lactis |
37 | - |
assay at | Enterococcus faecalis |
37 | - |
assay at | Streptococcus pneumoniae |
37 | - |
assay at | Corynebacterium glutamicum |
37 | - |
assay at | Streptococcus thermophilus |
37 | - |
assay at | Micromonospora griseorubida |
37 | - |
assay at | Rhodococcus corynebacterioides |
37 | - |
assay at | Streptomyces fradiae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Lactiplantibacillus plantarum |
7.5 | - |
assay at | Bacillus subtilis |
7.5 | - |
assay at | Priestia megaterium |
7.5 | - |
assay at | Lactococcus lactis |
7.5 | - |
assay at | Enterococcus faecalis |
7.5 | - |
assay at | Streptococcus pneumoniae |
7.5 | - |
assay at | Corynebacterium glutamicum |
7.5 | - |
assay at | Streptococcus thermophilus |
7.5 | - |
assay at | Micromonospora griseorubida |
7.5 | - |
assay at | Rhodococcus corynebacterioides |
7.5 | - |
assay at | Streptomyces fradiae |