Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA | Escherichia coli | YgdE is the 2'-O-methyltransferase responsible for modifying 23S rRNA nucleotide C2498. Modification occurs before assembly of the 50S ribosomal subunit | S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0ADR6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA | YgdE is the 2'-O-methyltransferase responsible for modifying 23S rRNA nucleotide C2498. Modification occurs before assembly of the 50S ribosomal subunit | Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA | - |
? | |
S-adenosyl-L-methionine + cytidine2498 in 23S rRNA | the recombinant YgdE methyltransferase modifies C2498 in naked 23S rRNA, but not in assembled 50S subunits or ribosomes. Nucleotide C2498 is situated within a highly conserved and heavily modified rRNA sequence, and the activity of YgdE is influenced by other modification enzymes that target this region | Escherichia coli | S-adenosyl-L-homocysteine + 2'-O-methylcytidine2498 in 23S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rRNA large subunit methyltransferase M | - |
Escherichia coli |
ygdE | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |
General Information | Comment | Organism |
---|---|---|
malfunction | C2498 methylation and the putative second methylation observed in the wild-type (YgdE+) strain are completely absent in the DygdE strain. Inactivation of the ygdE gene leads to loss of methylation at nucleotide C2498. The loss of ygdE function causes a slight reduction in bacterial fitness | Escherichia coli |