Cloned (Comment) | Organism |
---|---|
the expression of ErmC' by translational coupling to kdsB, under the control of the T7lac promoter. The pTERM31 plasmid is transformed into Escherichia coli strain BL219(DE3)/pLysS | Bacillus subtilis |
Crystallization (Comment) | Organism |
---|---|
crystals of ErmC' are obtained by the hanging-drop vapor diffusion method. Crystal structure of ErmC' (a naturally occurring variant of ErmC) determined at 3.0 A resolution by multiple anomalous diffraction phasing methods. The structure consists of a conserved alpha/beta amino-terminal domain which binds the cofactor S-adenosyl-L-methionine, followed by a smaller, alpha-helical RNA-recognition domain | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P13956 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 S-adenosyl-L-methionine + adenine2085 in 23S rRNA | - |
Bacillus subtilis | 2 S-adenosyl-L-homocysteine + N6-dimethyladenine2085 in 23S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ErmC' | a naturally occuring variant of ErmC | Bacillus subtilis |