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Literature summary for 2.1.1.180 extracted from

  • Savic, M.; Sunita, S.; Zelinskaya, N.; Desai, P.M.; Macmaster, R.; Vinal, K.; Conn, G.L.
    30S Subunit-dependent activation of the Sorangium cellulosum So ce56 aminoglycoside resistance-conferring 16S rRNA methyltransferase Kmr (2015), Antimicrob. Agents Chemother., 59, 2807-2816 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.1.1.180

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Sorangium cellulosum

Crystallization (Commentary)

Crystallization (Comment) Organism
apo-enzyme, to 1.8 A resolution, by molecular replacement. Kmr possesses a canonical S-adenosylmethionine binding pocket but with reduced sensitivity to mutation Sorangium cellulosum

Protein Variants

Protein Variants Comment Organism
F144A mutation does not affect enzyme activity Sorangium cellulosum
R204A mutation does not affect enzyme activity Sorangium cellulosum
R205A mutation does not affect enzyme activity Sorangium cellulosum
R207A loss of activity Sorangium cellulosum
W107A loss of activity Sorangium cellulosum
W107A/F144A loss of activity Sorangium cellulosum
W107F mutant enzyme has a kanamycin MIC indistinguishable from that of wild-type Kmr Sorangium cellulosum
W107F/F144A loss of activity Sorangium cellulosum

Inhibitors

Inhibitors Comment Organism Structure
additional information not inhibitory: S-adenosylhomocysteine in up to 1000fold excess over S-adenosylmethionine Sorangium cellulosum

Organism

Organism UniProt Comment Textmining
Sorangium cellulosum B2L3G9
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information enzyme has low affinity for cosubstrate S-adenosylmethionine Sorangium cellulosum ?
-
-
S-adenosyl-L-methionine + adenine1408 in 16S rRNA
-
 Sorangium cellulosum S-adenosyl-L-homocysteine + N1-methyladenine1408 in 16S rRNA
-
 ?

Synonyms

Synonyms Comment Organism
Kmr
-
 Sorangium cellulosum

General Information

General Information Comment Organism
physiological function expression in Escherichia coli provides high-level resistance to kanamycin and apramycin but not to gentamicin Sorangium cellulosum