Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Catenulispora acidiphila |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.03 M Bis-Tris (pH 5.5) and 25.5% (w/v) polyethylene glycol 3350 | Catenulispora acidiphila |
Protein Variants | Comment | Organism |
---|---|---|
D21A | the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme | Catenulispora acidiphila |
D36A | inactive | Catenulispora acidiphila |
D61A | inactive | Catenulispora acidiphila |
E94A | the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme | Catenulispora acidiphila |
K115A | the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme | Catenulispora acidiphila |
R43A/R73A | the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme | Catenulispora acidiphila |
R66A | the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme | Catenulispora acidiphila |
S201A | the mutant shows increased binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme | Catenulispora acidiphila |
S202A | the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme | Catenulispora acidiphila |
W113A | the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme | Catenulispora acidiphila |
W113F | the mutant shows increased binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme | Catenulispora acidiphila |
W203A | the mutant shows increased binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme | Catenulispora acidiphila |
W203F | the mutant shows reduced binding affinity with S-adenosyl-L-methionine compared to the wild type enzyme | Catenulispora acidiphila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28600 | - |
x * 28600, full-length protein, SDS-PAGE | Catenulispora acidiphila |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine1408 in 16S rRNA | Catenulispora acidiphila | - |
S-adenosyl-L-homocysteine + N1-methyladenine1408 in 16S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Catenulispora acidiphila | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Catenulispora acidiphila |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + adenine1408 in 16S rRNA | - |
Catenulispora acidiphila | S-adenosyl-L-homocysteine + N1-methyladenine1408 in 16S rRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28600, full-length protein, SDS-PAGE | Catenulispora acidiphila |
Synonyms | Comment | Organism |
---|---|---|
16S rRNA (m1A1408) methyltransferase | - |
Catenulispora acidiphila |
KAM | - |
Catenulispora acidiphila |