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Literature summary for 2.1.1.179 extracted from

  • Schmitt, E.; Galimand, M.; Panvert, M.; Courvalin, P.; Mechulam, Y.
    Structural bases for 16 S rRNA methylation catalyzed by ArmA and RmtB methyltransferases (2009), J. Mol. Biol., 388, 570-582.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
-
Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
RmtB containing S-adenosyl-L-homocysteine in the active site Escherichia coli

Protein Variants

Protein Variants Comment Organism
E182A tobramycin MIC is identical with that of wild-type RmtB Escherichia coli
H50A tobramycin MIC is drastically reduced compared to wild-type enzyme Escherichia coli
H81A tobramycin MIC is identical with that of wild-type RmtB. No change in methylation activity compared to wild-type enzyme Escherichia coli
K14A tobramycin MIC is identical with that of wild-type RmtB. 36% of the methylation activity compared to wild-type enzyme Escherichia coli
K174A tobramycin MIC is drastically reduced compared to wild-type enzyme. 0.7% of the methylation activity compared to wild-type enzyme Escherichia coli
R17A tobramycin MIC is identical with that of wild-type RmtB Escherichia coli
R181A tobramycin MIC is drastically reduced compared to wild-type enzyme Escherichia coli
R48A tobramycin MIC is identical with that of wild-type RmtB Escherichia coli
S83A tobramycin MIC is identical with that of wild-type RmtB. 18% of the methylation activity compared to wild-type enzyme Escherichia coli
Y56F tobramycin MIC is identical with that of wild-type RmtB Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine1405 in 16S rRNA Escherichia coli post-transcriptional methylation of N7-G1405 in 16S rRNA of 30S ribosomal subunits S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
?

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
-
Escherichia coli S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
?
S-adenosyl-L-methionine + guanine1405 in 16S rRNA post-transcriptional methylation of N7-G1405 in 16S rRNA of 30S ribosomal subunits Escherichia coli S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
?

Synonyms

Synonyms Comment Organism
RmtB
-
Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Escherichia coli