Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
RmtB containing S-adenosyl-L-homocysteine in the active site | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
E182A | tobramycin MIC is identical with that of wild-type RmtB | Escherichia coli |
H50A | tobramycin MIC is drastically reduced compared to wild-type enzyme | Escherichia coli |
H81A | tobramycin MIC is identical with that of wild-type RmtB. No change in methylation activity compared to wild-type enzyme | Escherichia coli |
K14A | tobramycin MIC is identical with that of wild-type RmtB. 36% of the methylation activity compared to wild-type enzyme | Escherichia coli |
K174A | tobramycin MIC is drastically reduced compared to wild-type enzyme. 0.7% of the methylation activity compared to wild-type enzyme | Escherichia coli |
R17A | tobramycin MIC is identical with that of wild-type RmtB | Escherichia coli |
R181A | tobramycin MIC is drastically reduced compared to wild-type enzyme | Escherichia coli |
R48A | tobramycin MIC is identical with that of wild-type RmtB | Escherichia coli |
S83A | tobramycin MIC is identical with that of wild-type RmtB. 18% of the methylation activity compared to wild-type enzyme | Escherichia coli |
Y56F | tobramycin MIC is identical with that of wild-type RmtB | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine1405 in 16S rRNA | Escherichia coli | post-transcriptional methylation of N7-G1405 in 16S rRNA of 30S ribosomal subunits | S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine1405 in 16S rRNA | - |
Escherichia coli | S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine1405 in 16S rRNA | post-transcriptional methylation of N7-G1405 in 16S rRNA of 30S ribosomal subunits | Escherichia coli | S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
RmtB | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |