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Literature summary for 2.1.1.179 extracted from

  • Husain, N.; Tkaczuk, K.L.; Tulsidas, S.R.; Kaminska, K.H.; Cubrilo, S.; Maravic-Vlahovicek, G.; Bujnicki, J.M.; Sivaraman, J.
    Structural basis for the methylation of G1405 in 16S rRNA by aminoglycoside resistance methyltransferase Sgm from an antibiotic producer: a diversity of active sites in m7G methyltransferases (2010), Nucleic Acids Res., 38, 4120-4132.
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
cloning of the sgm gene into pET-25b (+) vector with the addition of N-terminal non-cleavable His6 tag, as well as alanine mutagenesis of residues D156, D182 and R108. The constructs are co-transformed along with pGroESL into the strain BL21 (DE3) of Escherichia coli for protein expression Micromonospora zionensis

Crystallization (Commentary)

Crystallization (Comment) Organism
purified Sgm is complexed with 5 mM of the cofactors S-adenosylmethionine/S-adenosylhomocysteine. Crystallization trials are carried out at room temperature by hanging-drop vapor-diffusion method, structure of Sgm in complex with cofactors S-adenosylmethionine and S-adenosylhomocysteine is determined at 2.0 A and 2.1 A resolution, respectively Micromonospora zionensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
S-adenosyl-L-methionine + guanine1405 in 16S rRNA Micromonospora zionensis the enzyme confers resistance to aminoglycosides like gentamicin and sisomicin by specifically methylating G1405 in bacterial 16S rRNA S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
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 ?

Organism

Organism UniProt Comment Textmining
Micromonospora zionensis Q7M0R2
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-

Purification (Commentary)

Purification (Comment) Organism
-
 Micromonospora zionensis

Reaction

Reaction Comment Organism Reaction ID
S-adenosyl-L-methionine + guanine1405 in 16S rRNA = S-adenosyl-L-homocysteine + N7-methylguanine1405 in 16S rRNA the mechanism of methylation of G1405 by Sgm is proposed and compared with other m7G methyltransferases Micromonospora zionensis
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
S-adenosyl-L-methionine + guanine1405 in 16S rRNA
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 Micromonospora zionensis S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
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 ?
S-adenosyl-L-methionine + guanine1405 in 16S rRNA the enzyme confers resistance to aminoglycosides like gentamicin and sisomicin by specifically methylating G1405 in bacterial 16S rRNA Micromonospora zionensis S-adenosyl-L-homocysteine + 7-methylguanine1405 in 16S rRNA
-
 ?

Synonyms

Synonyms Comment Organism
sgm
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 Micromonospora zionensis
sisomicin-gentamicin methyltransferase
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 Micromonospora zionensis