Literature summary for 2.1.1.174 extracted from

Osterman, I.A.; Sergiev, P.V.; Tsvetkov, P.O.; Makarov, A.A.; Bogdanov, A.A.; Dontsova, O.A.
Methylated 23S rRNA nucleotide m2G1835 of Escherichia coli ribosome facilitates subunit association (2011), Biochimie, 93, 725-729.

Search for more literature for 2.1.1.174

Protein Variants

Protein Variants	Comment	Organism
additional information	generation of rlmG knock-out cells, ribosomes purified from the rlmG knockout strain do not have methyl group attached to nucleotide G1835 of the 23S rRNA	Escherichia coli

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	required	Escherichia coli

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
S-adenosyl-L-methionine + guanine1835 in 23S rRNA	Escherichia coli	m2G1835 is located at ribosomal subunits 50S and 30S interface	S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA	-	?

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	gene rlmG	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
S-adenosyl-L-methionine + guanine1835 in 23S rRNA	-	Escherichia coli	S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA	-	?
S-adenosyl-L-methionine + guanine1835 in 23S rRNA	m2G1835 is located at ribosomal subunits 50S and 30S interface	Escherichia coli	S-adenosyl-L-homocysteine + N2-methylguanine1835 in 23S rRNA	-	?

Synonyms

Synonyms	Comment	Organism
N2-methyltransferase RlmG	-	Escherichia coli
RlmG	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
S-adenosyl-L-methionine	-	Escherichia coli

General Information

General Information	Comment	Organism
malfunction	absence of 23S rRNA nucleotide G1835 methylation does not influence the fidelity of translation or ribosome interaction with translation GTPases and decreases bacterial cell survival at osmotic and oxidative stress. Cells devoid of the rlmG gene are hypersensitive to osmotic and oxidative stress	Escherichia coli
physiological function	methylation of 23S rRNA nucleotide m2G1835 is important for association of ribosomal subunits. Methylation of G1835 provides a significant advantage for bacteria at osmotic and oxidative stress	Escherichia coli

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Release 2023.1 (January 2023)