Cloned (Comment) | Organism |
---|---|
- |
Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 2 mM, markedly reduces the level of methylation | Escherichia coli | |
Mg2+ | 6 mM, markedly reduces the level of methylation | Escherichia coli |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | close to unit stoichiometry of methylation can be achieved at 0.9 mM Mg2+ | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
37000 | - |
gel filtration | Escherichia coli |
37600 | - |
1 * 37600, calculated from sequence | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine1207 in 16S rRNA | Escherichia coli | because the m2G1207 methyltransferase reacts with 30S particles but barely at all with 16S RNA, it seems likely that methylation of the guanine residue occurs after the 16S RNA has associated with some ribosomal proteins | S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P39406 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
S-adenosyl-L-methionine + guanine1207 in 16S rRNA | because the m2G1207 methyltransferase reacts with 30S particles but barely at all with 16S RNA, it seems likely that methylation of the guanine residue occurs after the 16S RNA has associated with some ribosomal proteins | Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA | - |
? | |
S-adenosyl-L-methionine + guanine1207 in 16S rRNA | the enzyme reacts well with 30S subunits reconstituted from 16S RNA transcripts and 30S proteins but is almost inactive with the corresponding free RNA. Of the three naturally occurring N2-methylguanine residues, only N2-methylguanine1207 is formed. It is suggested that the optimal substrate may be a ribonucleoprotein particle less structured than a 30S ribosome but more so than free RNA. Localization of the site of methylation by hybridization-protection studies using deoxyoligomers that are complementary to the RNA sequence spanning each of the N2-methylguanine sites | Escherichia coli | S-adenosyl-L-homocysteine + N2-methylguanine1207 in 16S rRNA | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 37600, calculated from sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
16 S RNA m2G1207 methyltransferase | - |
Escherichia coli |
ribosomal RNA small subunit methyltransferase C | - |
Escherichia coli |
RsmC | - |
Escherichia coli |
yjjT | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Escherichia coli |