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Literature summary for 2.1.1.170 extracted from

  • Benitez-Paez, A.; Villarroya, M.; Armengod, M.E.
    Regulation of expression and catalytic activity of Escherichia coli RsmG methyltransferase (2012), RNA, 18, 795-806.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information activity of rsmGp slightly increases by fourfold when cells are grown in minimal media supplemented with glycerol instead of glucose Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
gene rsmG is the second member in a bicistronic operon, rsmG also has its own promoter, RsmG expression might depend on the activity of an inverted repeated region, located between the rsmG promoter and ribosome binding site, which works as a weak transcriptional terminator. Expression of C-terminally His6-tagged and FLAG-tagged RsmG in Escherichia coli TOP10 cells Escherichia coli

Protein Variants

Protein Variants Comment Organism
D56A site-directed mutagenesis of the catalytic residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme Escherichia coli
D71A site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and catalytically inactive Escherichia coli
D96A site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and catalytically inactive Escherichia coli
G73A site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-sensitive and shows reduced activity compared to the wild-type enzyme Escherichia coli
G75A site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme Escherichia coli
G77A site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and catalytically inactive Escherichia coli
H53A site-directed mutagenesis of the catalytic residue, the mutant is partly streptomycin-resistant and shows reduced activity compared to the wild-type enzyme Escherichia coli
K100A/R101A site-directed mutagenesis of the RNA-binding residues, the mutant is streptomycin-resistant and catalytically inactive Escherichia coli
K165A site-directed mutagenesis of the RNA binding residue, the mutant is streptomycin-sensitive, but shows reduced activity compared to the wild-type enzyme Escherichia coli
additional information a rsmG null mutant is streptomycin-resistant Escherichia coli
P79A site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme Escherichia coli
R123A site-directed mutagenesis of the RNA-binding residue, the mutant is streptomycin-sensitive, but shows reduced activity compared to the wild-type enzyme Escherichia coli
R139A site-directed mutagenesis of the catalytic residue, the mutant is streptomycin-resistant and catalytically inactive Escherichia coli
R139K site-directed mutagenesis of the catalytic residue, the mutant is partly streptomycin-resistant and shows reduced activity compared to the wild-type enzyme Escherichia coli
R197A site-directed mutagenesis of the RNA binding residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A6U5 gene rsmG encoded in the mnmG-rsmG operon
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Purification (Commentary)

Purification (Comment) Organism
recombinant FLAG-tagged RsmG from Escherichia coli TOP10 cells by affinity chromatography Escherichia coli

Source Tissue

Source Tissue Comment Organism Textmining
cell culture activity of rsmGp slightly increases by fourfold when cells are grown in minimal media supplemented with glycerol instead of glucose Escherichia coli
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Synonyms

Synonyms Comment Organism
rsmG
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Escherichia coli
RsmG methyltransferase
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Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Escherichia coli

Cofactor

Cofactor Comment Organism Structure
S-adenosyl-L-methionine
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Escherichia coli

Expression

Organism Comment Expression
Escherichia coli RsmG levels decrease under conditions that down-regulate rRNA synthesis, but coordination between rRNA and RsmG expression does not seem to occur at the level of transcription initiation down
Escherichia coli slight induction of rsmGp during stationary phase is independent of the stress-inducible sigma factor RpoS up

General Information

General Information Comment Organism
malfunction loss of the m7G527 modification confers low-level streptomycin resistance and may affect ribosomal functioning Escherichia coli
additional information positively charged residues on the protein surface around the active site, K100/R101, R123, K165, and R197, might play a role in the binding of the incoming 530 loop since their change to alanine impairs the modification function of RsmG Escherichia coli
physiological function RsmG is an S-adenosyl-L-methionine-dependent methyltransferase responsible for the synthesis of m7G527 in the 530 loop of bacterial 16S rRNA. This loop is universally conserved, plays a key role in ribosomal accuracy, and is a target for streptomycin binding, mechanisms controlling RsmG expression and activity, overview. Gene rsmG as part of a bicistronic operon also has its own promoter, which appears, in actively growing cells, as a control device to offset both the relatively low stability of RsmG and inhibition of the operon promoter. Critical importance of some residues located in the active site of Escherichia coli RsmG for the m7G modification process, the residues play a role in rRNA binding and catalysis Escherichia coli