Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | activity of rsmGp slightly increases by fourfold when cells are grown in minimal media supplemented with glycerol instead of glucose | Escherichia coli |
Cloned (Comment) | Organism |
---|---|
gene rsmG is the second member in a bicistronic operon, rsmG also has its own promoter, RsmG expression might depend on the activity of an inverted repeated region, located between the rsmG promoter and ribosome binding site, which works as a weak transcriptional terminator. Expression of C-terminally His6-tagged and FLAG-tagged RsmG in Escherichia coli TOP10 cells | Escherichia coli |
Protein Variants | Comment | Organism |
---|---|---|
D56A | site-directed mutagenesis of the catalytic residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme | Escherichia coli |
D71A | site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and catalytically inactive | Escherichia coli |
D96A | site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and catalytically inactive | Escherichia coli |
G73A | site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-sensitive and shows reduced activity compared to the wild-type enzyme | Escherichia coli |
G75A | site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme | Escherichia coli |
G77A | site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and catalytically inactive | Escherichia coli |
H53A | site-directed mutagenesis of the catalytic residue, the mutant is partly streptomycin-resistant and shows reduced activity compared to the wild-type enzyme | Escherichia coli |
K100A/R101A | site-directed mutagenesis of the RNA-binding residues, the mutant is streptomycin-resistant and catalytically inactive | Escherichia coli |
K165A | site-directed mutagenesis of the RNA binding residue, the mutant is streptomycin-sensitive, but shows reduced activity compared to the wild-type enzyme | Escherichia coli |
additional information | a rsmG null mutant is streptomycin-resistant | Escherichia coli |
P79A | site-directed mutagenesis of the S-adenosyl-L-methionine-binding residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme | Escherichia coli |
R123A | site-directed mutagenesis of the RNA-binding residue, the mutant is streptomycin-sensitive, but shows reduced activity compared to the wild-type enzyme | Escherichia coli |
R139A | site-directed mutagenesis of the catalytic residue, the mutant is streptomycin-resistant and catalytically inactive | Escherichia coli |
R139K | site-directed mutagenesis of the catalytic residue, the mutant is partly streptomycin-resistant and shows reduced activity compared to the wild-type enzyme | Escherichia coli |
R197A | site-directed mutagenesis of the RNA binding residue, the mutant is streptomycin-resistant and shows reduced activity compared to the wild-type enzyme | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P0A6U5 | gene rsmG encoded in the mnmG-rsmG operon | - |
Purification (Comment) | Organism |
---|---|
recombinant FLAG-tagged RsmG from Escherichia coli TOP10 cells by affinity chromatography | Escherichia coli |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | activity of rsmGp slightly increases by fourfold when cells are grown in minimal media supplemented with glycerol instead of glucose | Escherichia coli | - |
Synonyms | Comment | Organism |
---|---|---|
rsmG | - |
Escherichia coli |
RsmG methyltransferase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
S-adenosyl-L-methionine | - |
Escherichia coli |
Organism | Comment | Expression |
---|---|---|
Escherichia coli | RsmG levels decrease under conditions that down-regulate rRNA synthesis, but coordination between rRNA and RsmG expression does not seem to occur at the level of transcription initiation | down |
Escherichia coli | slight induction of rsmGp during stationary phase is independent of the stress-inducible sigma factor RpoS | up |
General Information | Comment | Organism |
---|---|---|
malfunction | loss of the m7G527 modification confers low-level streptomycin resistance and may affect ribosomal functioning | Escherichia coli |
additional information | positively charged residues on the protein surface around the active site, K100/R101, R123, K165, and R197, might play a role in the binding of the incoming 530 loop since their change to alanine impairs the modification function of RsmG | Escherichia coli |
physiological function | RsmG is an S-adenosyl-L-methionine-dependent methyltransferase responsible for the synthesis of m7G527 in the 530 loop of bacterial 16S rRNA. This loop is universally conserved, plays a key role in ribosomal accuracy, and is a target for streptomycin binding, mechanisms controlling RsmG expression and activity, overview. Gene rsmG as part of a bicistronic operon also has its own promoter, which appears, in actively growing cells, as a control device to offset both the relatively low stability of RsmG and inhibition of the operon promoter. Critical importance of some residues located in the active site of Escherichia coli RsmG for the m7G modification process, the residues play a role in rRNA binding and catalysis | Escherichia coli |